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Post‐translational modification directs nuclear and hyphal tip localization of C andida albicans mRNA‐binding protein Slr1

The morphological transition of the opportunistic fungal pathogen Candida albicans from budding to hyphal growth has been implicated in its ability to cause disease in animal models. Absence of SR‐like RNA‐binding protein Slr1 slows hyphal formation and decreases virulence in a systemic candidiasis...

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Autores principales: Ariyachet, Chaiyaboot, Beißel, Christian, Li, Xiang, Lorrey, Selena, Mackenzie, Olivia, Martin, Patrick M., O'Brien, Katharine, Pholcharee, Tossapol, Sim, Sue, Krebber, Heike, McBride, Anne E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405739/
https://www.ncbi.nlm.nih.gov/pubmed/28187496
http://dx.doi.org/10.1111/mmi.13643
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author Ariyachet, Chaiyaboot
Beißel, Christian
Li, Xiang
Lorrey, Selena
Mackenzie, Olivia
Martin, Patrick M.
O'Brien, Katharine
Pholcharee, Tossapol
Sim, Sue
Krebber, Heike
McBride, Anne E.
author_facet Ariyachet, Chaiyaboot
Beißel, Christian
Li, Xiang
Lorrey, Selena
Mackenzie, Olivia
Martin, Patrick M.
O'Brien, Katharine
Pholcharee, Tossapol
Sim, Sue
Krebber, Heike
McBride, Anne E.
author_sort Ariyachet, Chaiyaboot
collection PubMed
description The morphological transition of the opportunistic fungal pathogen Candida albicans from budding to hyphal growth has been implicated in its ability to cause disease in animal models. Absence of SR‐like RNA‐binding protein Slr1 slows hyphal formation and decreases virulence in a systemic candidiasis model, suggesting a role for post‐transcriptional regulation in these processes. SR (serine–arginine)‐rich proteins influence multiple steps in mRNA metabolism and their localization and function are frequently controlled by modification. We now demonstrate that Slr1 binds to polyadenylated RNA and that its intracellular localization is modulated by phosphorylation and methylation. Wildtype Slr1‐GFP is predominantly nuclear, but also co‐fractionates with translating ribosomes. The non‐phosphorylatable slr1‐6SA‐GFP protein, in which six serines in SR/RS clusters are substituted with alanines, primarily localizes to the cytoplasm in budding cells. Intriguingly, hyphal cells display a slr1‐6SA‐GFP focus at the tip near the Spitzenkörper, a vesicular structure involved in molecular trafficking to the tip. The presence of slr1‐6SA‐GFP hyphal tip foci is reduced in the absence of the mRNA‐transport protein She3, suggesting that unphosphorylated Slr1 associates with mRNA–protein complexes transported to the tip. The impact of SLR1 deletion on hyphal formation and function thus may be partially due to a role in hyphal mRNA transport.
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spelling pubmed-54057392018-05-01 Post‐translational modification directs nuclear and hyphal tip localization of C andida albicans mRNA‐binding protein Slr1 Ariyachet, Chaiyaboot Beißel, Christian Li, Xiang Lorrey, Selena Mackenzie, Olivia Martin, Patrick M. O'Brien, Katharine Pholcharee, Tossapol Sim, Sue Krebber, Heike McBride, Anne E. Mol Microbiol Research Articles The morphological transition of the opportunistic fungal pathogen Candida albicans from budding to hyphal growth has been implicated in its ability to cause disease in animal models. Absence of SR‐like RNA‐binding protein Slr1 slows hyphal formation and decreases virulence in a systemic candidiasis model, suggesting a role for post‐transcriptional regulation in these processes. SR (serine–arginine)‐rich proteins influence multiple steps in mRNA metabolism and their localization and function are frequently controlled by modification. We now demonstrate that Slr1 binds to polyadenylated RNA and that its intracellular localization is modulated by phosphorylation and methylation. Wildtype Slr1‐GFP is predominantly nuclear, but also co‐fractionates with translating ribosomes. The non‐phosphorylatable slr1‐6SA‐GFP protein, in which six serines in SR/RS clusters are substituted with alanines, primarily localizes to the cytoplasm in budding cells. Intriguingly, hyphal cells display a slr1‐6SA‐GFP focus at the tip near the Spitzenkörper, a vesicular structure involved in molecular trafficking to the tip. The presence of slr1‐6SA‐GFP hyphal tip foci is reduced in the absence of the mRNA‐transport protein She3, suggesting that unphosphorylated Slr1 associates with mRNA–protein complexes transported to the tip. The impact of SLR1 deletion on hyphal formation and function thus may be partially due to a role in hyphal mRNA transport. John Wiley and Sons Inc. 2017-03-01 2017-05 /pmc/articles/PMC5405739/ /pubmed/28187496 http://dx.doi.org/10.1111/mmi.13643 Text en © 2017 John Wiley & Sons Ltd This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Research Articles
Ariyachet, Chaiyaboot
Beißel, Christian
Li, Xiang
Lorrey, Selena
Mackenzie, Olivia
Martin, Patrick M.
O'Brien, Katharine
Pholcharee, Tossapol
Sim, Sue
Krebber, Heike
McBride, Anne E.
Post‐translational modification directs nuclear and hyphal tip localization of C andida albicans mRNA‐binding protein Slr1
title Post‐translational modification directs nuclear and hyphal tip localization of C andida albicans mRNA‐binding protein Slr1
title_full Post‐translational modification directs nuclear and hyphal tip localization of C andida albicans mRNA‐binding protein Slr1
title_fullStr Post‐translational modification directs nuclear and hyphal tip localization of C andida albicans mRNA‐binding protein Slr1
title_full_unstemmed Post‐translational modification directs nuclear and hyphal tip localization of C andida albicans mRNA‐binding protein Slr1
title_short Post‐translational modification directs nuclear and hyphal tip localization of C andida albicans mRNA‐binding protein Slr1
title_sort post‐translational modification directs nuclear and hyphal tip localization of c andida albicans mrna‐binding protein slr1
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405739/
https://www.ncbi.nlm.nih.gov/pubmed/28187496
http://dx.doi.org/10.1111/mmi.13643
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