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Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ

FtsZ is an essential protein for bacterial cell division, where it forms the cytoskeletal scaffold and may generate the constriction force. We have found previously that some mutant and foreign FtsZ that do not complement an ftsZ null can function for cell division in E. coli upon acquisition of a s...

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Autores principales: Gardner, Kiani A. J. Arkus, Osawa, Masaki, Erickson, Harold P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405962/
https://www.ncbi.nlm.nih.gov/pubmed/28445510
http://dx.doi.org/10.1371/journal.pone.0176643
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author Gardner, Kiani A. J. Arkus
Osawa, Masaki
Erickson, Harold P.
author_facet Gardner, Kiani A. J. Arkus
Osawa, Masaki
Erickson, Harold P.
author_sort Gardner, Kiani A. J. Arkus
collection PubMed
description FtsZ is an essential protein for bacterial cell division, where it forms the cytoskeletal scaffold and may generate the constriction force. We have found previously that some mutant and foreign FtsZ that do not complement an ftsZ null can function for cell division in E. coli upon acquisition of a suppressor mutation somewhere in the genome. We have now identified, via whole genome re-sequencing, single nucleotide polymorphisms in 11 different suppressor strains. Most of the mutations are in genes of various metabolic pathways, which may modulate cell division indirectly. Mutations in three genes, ispA, accD and nlpI, may be more directly involved in cell division. In addition to the genomic suppressor mutations, we identified intragenic suppressors of three FtsZ point mutants (R174A, E250K and L272V).
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spelling pubmed-54059622017-05-14 Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ Gardner, Kiani A. J. Arkus Osawa, Masaki Erickson, Harold P. PLoS One Research Article FtsZ is an essential protein for bacterial cell division, where it forms the cytoskeletal scaffold and may generate the constriction force. We have found previously that some mutant and foreign FtsZ that do not complement an ftsZ null can function for cell division in E. coli upon acquisition of a suppressor mutation somewhere in the genome. We have now identified, via whole genome re-sequencing, single nucleotide polymorphisms in 11 different suppressor strains. Most of the mutations are in genes of various metabolic pathways, which may modulate cell division indirectly. Mutations in three genes, ispA, accD and nlpI, may be more directly involved in cell division. In addition to the genomic suppressor mutations, we identified intragenic suppressors of three FtsZ point mutants (R174A, E250K and L272V). Public Library of Science 2017-04-26 /pmc/articles/PMC5405962/ /pubmed/28445510 http://dx.doi.org/10.1371/journal.pone.0176643 Text en © 2017 Gardner et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gardner, Kiani A. J. Arkus
Osawa, Masaki
Erickson, Harold P.
Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ
title Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ
title_full Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ
title_fullStr Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ
title_full_unstemmed Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ
title_short Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ
title_sort whole genome re-sequencing to identify suppressor mutations of mutant and foreign escherichia coli ftsz
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405962/
https://www.ncbi.nlm.nih.gov/pubmed/28445510
http://dx.doi.org/10.1371/journal.pone.0176643
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