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Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer
Addressing the transmission enigma of the neglected disease Buruli ulcer (BU) is a World Health Organization priority. In Australia, we have observed an association between mosquitoes harboring the causative agent, Mycobacterium ulcerans, and BU. Here we tested a contaminated skin model of BU transm...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406025/ https://www.ncbi.nlm.nih.gov/pubmed/28410412 http://dx.doi.org/10.1371/journal.pntd.0005553 |
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author | Wallace, John R. Mangas, Kirstie M. Porter, Jessica L. Marcsisin, Renee Pidot, Sacha J. Howden, Brian Omansen, Till F. Zeng, Weiguang Axford, Jason K. Johnson, Paul D. R. Stinear, Timothy P. |
author_facet | Wallace, John R. Mangas, Kirstie M. Porter, Jessica L. Marcsisin, Renee Pidot, Sacha J. Howden, Brian Omansen, Till F. Zeng, Weiguang Axford, Jason K. Johnson, Paul D. R. Stinear, Timothy P. |
author_sort | Wallace, John R. |
collection | PubMed |
description | Addressing the transmission enigma of the neglected disease Buruli ulcer (BU) is a World Health Organization priority. In Australia, we have observed an association between mosquitoes harboring the causative agent, Mycobacterium ulcerans, and BU. Here we tested a contaminated skin model of BU transmission by dipping the tails from healthy mice in cultures of the causative agent, Mycobacterium ulcerans. Tails were exposed to mosquito (Aedes notoscriptus and Aedes aegypti) blood feeding or punctured with sterile needles. Two of 12 of mice with M. ulcerans contaminated tails exposed to feeding A. notoscriptus mosquitoes developed BU. There were no mice exposed to A. aegypti that developed BU. Eighty-eight percent of mice (21/24) subjected to contaminated tail needle puncture developed BU. Mouse tails coated only in bacteria did not develop disease. A median incubation time of 12 weeks, consistent with data from human infections, was noted. We then specifically tested the M. ulcerans infectious dose-50 (ID50) in this contaminated skin surface infection model with needle puncture and observed an ID50 of 2.6 colony-forming units. We have uncovered a biologically plausible mechanical transmission mode of BU via natural or anthropogenic skin punctures. |
format | Online Article Text |
id | pubmed-5406025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54060252017-05-14 Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer Wallace, John R. Mangas, Kirstie M. Porter, Jessica L. Marcsisin, Renee Pidot, Sacha J. Howden, Brian Omansen, Till F. Zeng, Weiguang Axford, Jason K. Johnson, Paul D. R. Stinear, Timothy P. PLoS Negl Trop Dis Research Article Addressing the transmission enigma of the neglected disease Buruli ulcer (BU) is a World Health Organization priority. In Australia, we have observed an association between mosquitoes harboring the causative agent, Mycobacterium ulcerans, and BU. Here we tested a contaminated skin model of BU transmission by dipping the tails from healthy mice in cultures of the causative agent, Mycobacterium ulcerans. Tails were exposed to mosquito (Aedes notoscriptus and Aedes aegypti) blood feeding or punctured with sterile needles. Two of 12 of mice with M. ulcerans contaminated tails exposed to feeding A. notoscriptus mosquitoes developed BU. There were no mice exposed to A. aegypti that developed BU. Eighty-eight percent of mice (21/24) subjected to contaminated tail needle puncture developed BU. Mouse tails coated only in bacteria did not develop disease. A median incubation time of 12 weeks, consistent with data from human infections, was noted. We then specifically tested the M. ulcerans infectious dose-50 (ID50) in this contaminated skin surface infection model with needle puncture and observed an ID50 of 2.6 colony-forming units. We have uncovered a biologically plausible mechanical transmission mode of BU via natural or anthropogenic skin punctures. Public Library of Science 2017-04-14 /pmc/articles/PMC5406025/ /pubmed/28410412 http://dx.doi.org/10.1371/journal.pntd.0005553 Text en © 2017 Wallace et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wallace, John R. Mangas, Kirstie M. Porter, Jessica L. Marcsisin, Renee Pidot, Sacha J. Howden, Brian Omansen, Till F. Zeng, Weiguang Axford, Jason K. Johnson, Paul D. R. Stinear, Timothy P. Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer |
title | Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer |
title_full | Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer |
title_fullStr | Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer |
title_full_unstemmed | Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer |
title_short | Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer |
title_sort | mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of buruli ulcer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406025/ https://www.ncbi.nlm.nih.gov/pubmed/28410412 http://dx.doi.org/10.1371/journal.pntd.0005553 |
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