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High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles

Invertase immobilized on magnetic diatomaceous earth nanoparticles (mDE-APTES-invertase) with high sucrolytic activity was obtained by an easy and low-cost method. An experimental design was carried out to investigate the best immobilization conditions and it allowed obtaining an immobilized derivat...

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Autores principales: Cabrera, Mariana P., Assis, Caio R.D., Neri, David F.M., Pereira, Claudete F., Soria, Fernando, Carvalho, Luiz B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406527/
https://www.ncbi.nlm.nih.gov/pubmed/28480172
http://dx.doi.org/10.1016/j.btre.2017.03.001
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author Cabrera, Mariana P.
Assis, Caio R.D.
Neri, David F.M.
Pereira, Claudete F.
Soria, Fernando
Carvalho, Luiz B.
author_facet Cabrera, Mariana P.
Assis, Caio R.D.
Neri, David F.M.
Pereira, Claudete F.
Soria, Fernando
Carvalho, Luiz B.
author_sort Cabrera, Mariana P.
collection PubMed
description Invertase immobilized on magnetic diatomaceous earth nanoparticles (mDE-APTES-invertase) with high sucrolytic activity was obtained by an easy and low-cost method. An experimental design was carried out to investigate the best immobilization conditions and it allowed obtaining an immobilized derivative with a residual specific activity equal to 92.5%. Then, a second experimental design selected the mDE-APTES-invertase with higher specific activity in relation to other derivatives reported in the literature (2.42-fold). Thermal and storage stability for immobilized invertase were found to be 35 °C for 60 min (85% retained activity) and 120 days storage period (80% retained activity), respectively. Besides, a residual activity higher than 60% and 50% were observed for mDE-APTES-invertase after reuse in short and long term, respectively. Given the simple and efficient method to obtain an immobilized derivative with high activity, the mDE nanoparticles appear to be a promising matrix for invertase immobilization as well as for other biomolecules.
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spelling pubmed-54065272017-05-05 High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles Cabrera, Mariana P. Assis, Caio R.D. Neri, David F.M. Pereira, Claudete F. Soria, Fernando Carvalho, Luiz B. Biotechnol Rep (Amst) Article Invertase immobilized on magnetic diatomaceous earth nanoparticles (mDE-APTES-invertase) with high sucrolytic activity was obtained by an easy and low-cost method. An experimental design was carried out to investigate the best immobilization conditions and it allowed obtaining an immobilized derivative with a residual specific activity equal to 92.5%. Then, a second experimental design selected the mDE-APTES-invertase with higher specific activity in relation to other derivatives reported in the literature (2.42-fold). Thermal and storage stability for immobilized invertase were found to be 35 °C for 60 min (85% retained activity) and 120 days storage period (80% retained activity), respectively. Besides, a residual activity higher than 60% and 50% were observed for mDE-APTES-invertase after reuse in short and long term, respectively. Given the simple and efficient method to obtain an immobilized derivative with high activity, the mDE nanoparticles appear to be a promising matrix for invertase immobilization as well as for other biomolecules. Elsevier 2017-04-06 /pmc/articles/PMC5406527/ /pubmed/28480172 http://dx.doi.org/10.1016/j.btre.2017.03.001 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Cabrera, Mariana P.
Assis, Caio R.D.
Neri, David F.M.
Pereira, Claudete F.
Soria, Fernando
Carvalho, Luiz B.
High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles
title High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles
title_full High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles
title_fullStr High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles
title_full_unstemmed High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles
title_short High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles
title_sort high sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406527/
https://www.ncbi.nlm.nih.gov/pubmed/28480172
http://dx.doi.org/10.1016/j.btre.2017.03.001
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