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Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars

The shiitake mushroom (Lentinula edodes) is one of the most popular edible mushrooms in the world and has attracted attention for its value in medicinal and pharmacological uses. With recent advanced research and techniques, the agricultural cultivation of the shiitake mushroom has been greatly incr...

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Autores principales: Lee, Hwa-Yong, Moon, Suyun, Shim, Donghwan, Hong, Chang Pyo, Lee, Yi, Koo, Chang-Duck, Chung, Jong-Wook, Ryu, Hojin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406856/
https://www.ncbi.nlm.nih.gov/pubmed/28338645
http://dx.doi.org/10.3390/genes8040109
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author Lee, Hwa-Yong
Moon, Suyun
Shim, Donghwan
Hong, Chang Pyo
Lee, Yi
Koo, Chang-Duck
Chung, Jong-Wook
Ryu, Hojin
author_facet Lee, Hwa-Yong
Moon, Suyun
Shim, Donghwan
Hong, Chang Pyo
Lee, Yi
Koo, Chang-Duck
Chung, Jong-Wook
Ryu, Hojin
author_sort Lee, Hwa-Yong
collection PubMed
description The shiitake mushroom (Lentinula edodes) is one of the most popular edible mushrooms in the world and has attracted attention for its value in medicinal and pharmacological uses. With recent advanced research and techniques, the agricultural cultivation of the shiitake mushroom has been greatly increased, especially in East Asia. Additionally, demand for the development of new cultivars with good agricultural traits has been greatly enhanced, but the development processes are complicated and more challenging than for other edible mushrooms. In this study, we developed 44 novel polymorphic simple sequence repeat (SSR) markers for the determination of shiitake mushroom cultivars based on a whole genome sequencing database of L. edodes. These markers were found to be polymorphic and reliable when screened in 23 shiitake mushroom cultivars. For the 44 SSR markers developed in this study, the major allele frequency ranged from 0.13 to 0.94; the number of genotypes and number of alleles were each 2–11; the observed and expected heterozygosity were 0.00–1.00 and 0.10–0.90, respectively; and the polymorphic information content value ranged from 0.10 to 0.89. These new markers can be used for molecular breeding, the determination of cultivars, and other applications.
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spelling pubmed-54068562017-04-27 Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars Lee, Hwa-Yong Moon, Suyun Shim, Donghwan Hong, Chang Pyo Lee, Yi Koo, Chang-Duck Chung, Jong-Wook Ryu, Hojin Genes (Basel) Communication The shiitake mushroom (Lentinula edodes) is one of the most popular edible mushrooms in the world and has attracted attention for its value in medicinal and pharmacological uses. With recent advanced research and techniques, the agricultural cultivation of the shiitake mushroom has been greatly increased, especially in East Asia. Additionally, demand for the development of new cultivars with good agricultural traits has been greatly enhanced, but the development processes are complicated and more challenging than for other edible mushrooms. In this study, we developed 44 novel polymorphic simple sequence repeat (SSR) markers for the determination of shiitake mushroom cultivars based on a whole genome sequencing database of L. edodes. These markers were found to be polymorphic and reliable when screened in 23 shiitake mushroom cultivars. For the 44 SSR markers developed in this study, the major allele frequency ranged from 0.13 to 0.94; the number of genotypes and number of alleles were each 2–11; the observed and expected heterozygosity were 0.00–1.00 and 0.10–0.90, respectively; and the polymorphic information content value ranged from 0.10 to 0.89. These new markers can be used for molecular breeding, the determination of cultivars, and other applications. MDPI 2017-03-24 /pmc/articles/PMC5406856/ /pubmed/28338645 http://dx.doi.org/10.3390/genes8040109 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Lee, Hwa-Yong
Moon, Suyun
Shim, Donghwan
Hong, Chang Pyo
Lee, Yi
Koo, Chang-Duck
Chung, Jong-Wook
Ryu, Hojin
Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars
title Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars
title_full Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars
title_fullStr Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars
title_full_unstemmed Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars
title_short Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars
title_sort development of 44 novel polymorphic ssr markers for determination of shiitake mushroom (lentinula edodes) cultivars
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406856/
https://www.ncbi.nlm.nih.gov/pubmed/28338645
http://dx.doi.org/10.3390/genes8040109
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