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Impact of temperature and soil type on Mycobacterium bovis survival in the environment
Mycobacterium bovis, the causative agent of the bovine tuberculosis (bTB), mainly affects cattle, its natural reservoir, but also a wide range of domestic and wild mammals. Besides direct transmission via contaminated aerosols, indirect transmission of the M. bovis between wildlife and livestock mig...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407823/ https://www.ncbi.nlm.nih.gov/pubmed/28448585 http://dx.doi.org/10.1371/journal.pone.0176315 |
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author | Barbier, Elodie Rochelet, Murielle Gal, Laurent Boschiroli, Maria Laura Hartmann, Alain |
author_facet | Barbier, Elodie Rochelet, Murielle Gal, Laurent Boschiroli, Maria Laura Hartmann, Alain |
author_sort | Barbier, Elodie |
collection | PubMed |
description | Mycobacterium bovis, the causative agent of the bovine tuberculosis (bTB), mainly affects cattle, its natural reservoir, but also a wide range of domestic and wild mammals. Besides direct transmission via contaminated aerosols, indirect transmission of the M. bovis between wildlife and livestock might occur by inhalation or ingestion of environmental substrates contaminated through infected animal shedding. We monitored the survival of M. bovis in two soil samples chosen for their contrasted physical and-chemical properties (i.e. pH, clay content). The population of M. bovis spiked in sterile soils was enumerated by a culture-based method after 14, 30, 60, 90, 120 and 150 days of incubation at 4°C and 22°C. A qPCR based assay targeting the IS1561’ locus was also performed to monitor M. bovis in both sterile and biotic spiked soils. The analysis of survival profiles using culture-based method showed that M. bovis survived longer at lower temperature (4°C versus 22°C) whereas the impact of soil characteristics on M. bovis persistence was not obvious. Furthermore, qPCR-based assay detected M. bovis for a longer period of time than the culture based method with higher gene copy numbers observed in sterile soils than in biotic ones. Impact of soil type on M. bovis persistence need to be deepened in order to fill the gap of knowledge concerning indirect transmission of the disease. |
format | Online Article Text |
id | pubmed-5407823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54078232017-05-14 Impact of temperature and soil type on Mycobacterium bovis survival in the environment Barbier, Elodie Rochelet, Murielle Gal, Laurent Boschiroli, Maria Laura Hartmann, Alain PLoS One Research Article Mycobacterium bovis, the causative agent of the bovine tuberculosis (bTB), mainly affects cattle, its natural reservoir, but also a wide range of domestic and wild mammals. Besides direct transmission via contaminated aerosols, indirect transmission of the M. bovis between wildlife and livestock might occur by inhalation or ingestion of environmental substrates contaminated through infected animal shedding. We monitored the survival of M. bovis in two soil samples chosen for their contrasted physical and-chemical properties (i.e. pH, clay content). The population of M. bovis spiked in sterile soils was enumerated by a culture-based method after 14, 30, 60, 90, 120 and 150 days of incubation at 4°C and 22°C. A qPCR based assay targeting the IS1561’ locus was also performed to monitor M. bovis in both sterile and biotic spiked soils. The analysis of survival profiles using culture-based method showed that M. bovis survived longer at lower temperature (4°C versus 22°C) whereas the impact of soil characteristics on M. bovis persistence was not obvious. Furthermore, qPCR-based assay detected M. bovis for a longer period of time than the culture based method with higher gene copy numbers observed in sterile soils than in biotic ones. Impact of soil type on M. bovis persistence need to be deepened in order to fill the gap of knowledge concerning indirect transmission of the disease. Public Library of Science 2017-04-27 /pmc/articles/PMC5407823/ /pubmed/28448585 http://dx.doi.org/10.1371/journal.pone.0176315 Text en © 2017 Barbier et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Barbier, Elodie Rochelet, Murielle Gal, Laurent Boschiroli, Maria Laura Hartmann, Alain Impact of temperature and soil type on Mycobacterium bovis survival in the environment |
title | Impact of temperature and soil type on Mycobacterium bovis survival in the environment |
title_full | Impact of temperature and soil type on Mycobacterium bovis survival in the environment |
title_fullStr | Impact of temperature and soil type on Mycobacterium bovis survival in the environment |
title_full_unstemmed | Impact of temperature and soil type on Mycobacterium bovis survival in the environment |
title_short | Impact of temperature and soil type on Mycobacterium bovis survival in the environment |
title_sort | impact of temperature and soil type on mycobacterium bovis survival in the environment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407823/ https://www.ncbi.nlm.nih.gov/pubmed/28448585 http://dx.doi.org/10.1371/journal.pone.0176315 |
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