Cargando…

Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation

As glutathione (GSH) plays an essential role in growth and symbiotic capacity of rhizobia, a glutathione synthetase (gshB) mutant of Rhizobium leguminosarum biovar viciae 3841 (Rlv3841) was characterised. It fails to efficiently utilise various compounds as a sole carbon source, including glucose, s...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheng, Guojun, Karunakaran, Ramakrishnan, East, Alison K., Munoz-Azcarate, Olaya, Poole, Philip S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407991/
https://www.ncbi.nlm.nih.gov/pubmed/28333211
http://dx.doi.org/10.1093/femsle/fnx045
_version_ 1783232209750065152
author Cheng, Guojun
Karunakaran, Ramakrishnan
East, Alison K.
Munoz-Azcarate, Olaya
Poole, Philip S.
author_facet Cheng, Guojun
Karunakaran, Ramakrishnan
East, Alison K.
Munoz-Azcarate, Olaya
Poole, Philip S.
author_sort Cheng, Guojun
collection PubMed
description As glutathione (GSH) plays an essential role in growth and symbiotic capacity of rhizobia, a glutathione synthetase (gshB) mutant of Rhizobium leguminosarum biovar viciae 3841 (Rlv3841) was characterised. It fails to efficiently utilise various compounds as a sole carbon source, including glucose, succinate, glutamine and histidine, and shows 60%–69% reduction in uptake rates of glucose, succinate and the non-metabolisable substrate α-amino isobutyric acid. The defect in glucose uptake can be overcome by addition of exogenous GSH, indicating GSH, but not its bacterial synthesis, is required for efficient transport. GSH is not involved in the regulation of the activity of Rlv3841's transporters via the global regulator of transport, Pts(NTR). Although lack of GSH reduces transcription of the branched amino acid transporter, this was not the case for all uptake transport systems, for example, the amino acid permease. This suggests GSH alters activity and/or assembly of transport systems by an unknown mechanism. In interaction with plants, the gshB mutant is not only severely impaired in rhizosphere colonisation, but also shows a 50% reduction in dry weight of plants and nitrogen-fixation ability. This reveals that changes in GSH metabolism affect the bacterial–plant interactions required for symbiosis.
format Online
Article
Text
id pubmed-5407991
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-54079912017-05-03 Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation Cheng, Guojun Karunakaran, Ramakrishnan East, Alison K. Munoz-Azcarate, Olaya Poole, Philip S. FEMS Microbiol Lett Research Letter As glutathione (GSH) plays an essential role in growth and symbiotic capacity of rhizobia, a glutathione synthetase (gshB) mutant of Rhizobium leguminosarum biovar viciae 3841 (Rlv3841) was characterised. It fails to efficiently utilise various compounds as a sole carbon source, including glucose, succinate, glutamine and histidine, and shows 60%–69% reduction in uptake rates of glucose, succinate and the non-metabolisable substrate α-amino isobutyric acid. The defect in glucose uptake can be overcome by addition of exogenous GSH, indicating GSH, but not its bacterial synthesis, is required for efficient transport. GSH is not involved in the regulation of the activity of Rlv3841's transporters via the global regulator of transport, Pts(NTR). Although lack of GSH reduces transcription of the branched amino acid transporter, this was not the case for all uptake transport systems, for example, the amino acid permease. This suggests GSH alters activity and/or assembly of transport systems by an unknown mechanism. In interaction with plants, the gshB mutant is not only severely impaired in rhizosphere colonisation, but also shows a 50% reduction in dry weight of plants and nitrogen-fixation ability. This reveals that changes in GSH metabolism affect the bacterial–plant interactions required for symbiosis. Oxford University Press 2017-02-23 2017-04 /pmc/articles/PMC5407991/ /pubmed/28333211 http://dx.doi.org/10.1093/femsle/fnx045 Text en © FEMS 2017. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Letter
Cheng, Guojun
Karunakaran, Ramakrishnan
East, Alison K.
Munoz-Azcarate, Olaya
Poole, Philip S.
Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation
title Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation
title_full Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation
title_fullStr Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation
title_full_unstemmed Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation
title_short Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation
title_sort glutathione affects the transport activity of rhizobium leguminosarum 3841 and is essential for efficient nodulation
topic Research Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407991/
https://www.ncbi.nlm.nih.gov/pubmed/28333211
http://dx.doi.org/10.1093/femsle/fnx045
work_keys_str_mv AT chengguojun glutathioneaffectsthetransportactivityofrhizobiumleguminosarum3841andisessentialforefficientnodulation
AT karunakaranramakrishnan glutathioneaffectsthetransportactivityofrhizobiumleguminosarum3841andisessentialforefficientnodulation
AT eastalisonk glutathioneaffectsthetransportactivityofrhizobiumleguminosarum3841andisessentialforefficientnodulation
AT munozazcarateolaya glutathioneaffectsthetransportactivityofrhizobiumleguminosarum3841andisessentialforefficientnodulation
AT poolephilips glutathioneaffectsthetransportactivityofrhizobiumleguminosarum3841andisessentialforefficientnodulation