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Adsorption of Ten Microcystin Congeners to Common Laboratory-Ware Is Solvent and Surface Dependent
Cyanobacteria can produce heptapetides called microcystins (MC) which are harmful to humans due to their ability to inhibit cellular protein phosphatases. Quantitation of these toxins can be hampered by their adsorption to common laboratory-ware during sample processing and analysis. Because of thei...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408203/ https://www.ncbi.nlm.nih.gov/pubmed/28383495 http://dx.doi.org/10.3390/toxins9040129 |
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author | Altaner, Stefan Puddick, Jonathan Wood, Susanna A. Dietrich, Daniel R. |
author_facet | Altaner, Stefan Puddick, Jonathan Wood, Susanna A. Dietrich, Daniel R. |
author_sort | Altaner, Stefan |
collection | PubMed |
description | Cyanobacteria can produce heptapetides called microcystins (MC) which are harmful to humans due to their ability to inhibit cellular protein phosphatases. Quantitation of these toxins can be hampered by their adsorption to common laboratory-ware during sample processing and analysis. Because of their structural diversity (>100 congeners) and different physico-chemical properties, they vary in their adsorption to surfaces. In this study, the adsorption of ten different MC congeners (encompassing non-arginated to doubly-arginated congeners) to common laboratory-ware was assessed using different solvent combinations. Sample handling steps were mimicked with glass and polypropylene pipettes and vials with increasing methanol concentrations at two pH levels, before analysis by liquid chromatography-tandem mass spectrometry. We demonstrated that MC adsorb to polypropylene surfaces irrespective of pH. After eight successive pipet actions using polypropylene tips ca. 20% of the MC were lost to the surface material, which increased to 25%–40% when solutions were acidified. The observed loss was alleviated by changing the methanol (MeOH) concentration in the final solvent. The required MeOH concentration varied depending on which congener was present. Microcystins only adsorbed to glass pipettes (loss up to 30% after eight pipet actions) when in acidified aqueous solutions. The latter appeared largely dependent on the presence of ionizable groups, such as arginine residues. |
format | Online Article Text |
id | pubmed-5408203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54082032017-05-03 Adsorption of Ten Microcystin Congeners to Common Laboratory-Ware Is Solvent and Surface Dependent Altaner, Stefan Puddick, Jonathan Wood, Susanna A. Dietrich, Daniel R. Toxins (Basel) Article Cyanobacteria can produce heptapetides called microcystins (MC) which are harmful to humans due to their ability to inhibit cellular protein phosphatases. Quantitation of these toxins can be hampered by their adsorption to common laboratory-ware during sample processing and analysis. Because of their structural diversity (>100 congeners) and different physico-chemical properties, they vary in their adsorption to surfaces. In this study, the adsorption of ten different MC congeners (encompassing non-arginated to doubly-arginated congeners) to common laboratory-ware was assessed using different solvent combinations. Sample handling steps were mimicked with glass and polypropylene pipettes and vials with increasing methanol concentrations at two pH levels, before analysis by liquid chromatography-tandem mass spectrometry. We demonstrated that MC adsorb to polypropylene surfaces irrespective of pH. After eight successive pipet actions using polypropylene tips ca. 20% of the MC were lost to the surface material, which increased to 25%–40% when solutions were acidified. The observed loss was alleviated by changing the methanol (MeOH) concentration in the final solvent. The required MeOH concentration varied depending on which congener was present. Microcystins only adsorbed to glass pipettes (loss up to 30% after eight pipet actions) when in acidified aqueous solutions. The latter appeared largely dependent on the presence of ionizable groups, such as arginine residues. MDPI 2017-04-06 /pmc/articles/PMC5408203/ /pubmed/28383495 http://dx.doi.org/10.3390/toxins9040129 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Altaner, Stefan Puddick, Jonathan Wood, Susanna A. Dietrich, Daniel R. Adsorption of Ten Microcystin Congeners to Common Laboratory-Ware Is Solvent and Surface Dependent |
title | Adsorption of Ten Microcystin Congeners to Common Laboratory-Ware Is Solvent and Surface Dependent |
title_full | Adsorption of Ten Microcystin Congeners to Common Laboratory-Ware Is Solvent and Surface Dependent |
title_fullStr | Adsorption of Ten Microcystin Congeners to Common Laboratory-Ware Is Solvent and Surface Dependent |
title_full_unstemmed | Adsorption of Ten Microcystin Congeners to Common Laboratory-Ware Is Solvent and Surface Dependent |
title_short | Adsorption of Ten Microcystin Congeners to Common Laboratory-Ware Is Solvent and Surface Dependent |
title_sort | adsorption of ten microcystin congeners to common laboratory-ware is solvent and surface dependent |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408203/ https://www.ncbi.nlm.nih.gov/pubmed/28383495 http://dx.doi.org/10.3390/toxins9040129 |
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