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Accurate identification of single nucleotide variants in whole genome amplified single cells

Genome-wide, DNA mutation analysis in single cells is prone to artifacts associated with cell lysis and whole genome amplification. Here we addressed these issues by developing Single-Cell Multiple Displacement Amplification (SCMDA) and the single-cell variant caller, SCcaller. Validated by comparin...

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Detalles Bibliográficos
Autores principales:	Dong, Xiao, Zhang, Lei, Milholland, Brandon, Lee, Moonsook, Maslov, Alexander Y., Wang, Tao, Vijg, Jan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408311/ https://www.ncbi.nlm.nih.gov/pubmed/28319112 http://dx.doi.org/10.1038/nmeth.4227

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