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Anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo
BACKGROUND: Resveratrol and curcumin, as natural flavones products, have good therapeutic effect in acute and chronic inflammation; on the other hand, tetramethylpyrazine (TMP) has angiogenesis and vessel protection effect as well as anti-inflammatory function. In this paper, the anti-inflammatory e...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408375/ https://www.ncbi.nlm.nih.gov/pubmed/28449676 http://dx.doi.org/10.1186/s12906-017-1739-7 |
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author | Chen, Long Liu, Tianjun Wang, Qiangsong Liu, Juan |
author_facet | Chen, Long Liu, Tianjun Wang, Qiangsong Liu, Juan |
author_sort | Chen, Long |
collection | PubMed |
description | BACKGROUND: Resveratrol and curcumin, as natural flavones products, have good therapeutic effect in acute and chronic inflammation; on the other hand, tetramethylpyrazine (TMP) has angiogenesis and vessel protection effect as well as anti-inflammatory function. In this paper, the anti-inflammatory effect of the tetramethylpyrazine, resveratrol and curcumin (TRC) combination in acute and chronic inflammation was reported in vivo. METHODS: The dose of the combined three natural products was optimized based on the acute paw swelling mouse model with a Uniform Design methodology. The therapeutic effect of TRC combination on chronic inflammation was investigated by using the collagen-induced arthritis (CIA) rat model based upon the following indexes: the volume of paw swelling, arthritis score, serum mediators and histological examination as well as immunohistochemical staining. The levels of alanine aminotransferase (ALT) and aspartate transaminase (AST) in serum were measured and the pathological sections of liver and kidney were analysed. LD(50) was measured based on the acute oral toxicity (AOT) standard method. RESULTS: The best formulation was the three components combined at the same mass proportion revealed by the Uniform Design methodology. This combination could significantly reduce the paw swelling in acute paw swelling mouse model, could reduce paw swelling and alleviate the damage in joint structural of ankle, cartilages and fibrous tissue in CIA rat model. The dose relationship was clear in both cases. Immunohistochemical staining of ankle tissue revealed that TRC combination was able to inhibit the expression of NF-κB p65 and TNF-α which were closely related to the inflammatory process. Analysis of serum mediators revealed TRC combination could inhibit the production of TNF-α, IL-1β, and IL-6 in the serum. Toxic study revealed this formulation was low toxic, LD(50) was larger than 5 g/kg, both the level of ALT and AST and histopathology in the liver and kidney exhibited no distinctions between the TRC combination and the blank group, no mortality occurred at the administered doses of 5 g/kg. CONCLUSIONS: The results showed this formulation could provide a novel potent treatment for acute and chronic inflammation (RA) without side effect like gastric injury occurring in NSAIDs. |
format | Online Article Text |
id | pubmed-5408375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54083752017-05-02 Anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo Chen, Long Liu, Tianjun Wang, Qiangsong Liu, Juan BMC Complement Altern Med Research Article BACKGROUND: Resveratrol and curcumin, as natural flavones products, have good therapeutic effect in acute and chronic inflammation; on the other hand, tetramethylpyrazine (TMP) has angiogenesis and vessel protection effect as well as anti-inflammatory function. In this paper, the anti-inflammatory effect of the tetramethylpyrazine, resveratrol and curcumin (TRC) combination in acute and chronic inflammation was reported in vivo. METHODS: The dose of the combined three natural products was optimized based on the acute paw swelling mouse model with a Uniform Design methodology. The therapeutic effect of TRC combination on chronic inflammation was investigated by using the collagen-induced arthritis (CIA) rat model based upon the following indexes: the volume of paw swelling, arthritis score, serum mediators and histological examination as well as immunohistochemical staining. The levels of alanine aminotransferase (ALT) and aspartate transaminase (AST) in serum were measured and the pathological sections of liver and kidney were analysed. LD(50) was measured based on the acute oral toxicity (AOT) standard method. RESULTS: The best formulation was the three components combined at the same mass proportion revealed by the Uniform Design methodology. This combination could significantly reduce the paw swelling in acute paw swelling mouse model, could reduce paw swelling and alleviate the damage in joint structural of ankle, cartilages and fibrous tissue in CIA rat model. The dose relationship was clear in both cases. Immunohistochemical staining of ankle tissue revealed that TRC combination was able to inhibit the expression of NF-κB p65 and TNF-α which were closely related to the inflammatory process. Analysis of serum mediators revealed TRC combination could inhibit the production of TNF-α, IL-1β, and IL-6 in the serum. Toxic study revealed this formulation was low toxic, LD(50) was larger than 5 g/kg, both the level of ALT and AST and histopathology in the liver and kidney exhibited no distinctions between the TRC combination and the blank group, no mortality occurred at the administered doses of 5 g/kg. CONCLUSIONS: The results showed this formulation could provide a novel potent treatment for acute and chronic inflammation (RA) without side effect like gastric injury occurring in NSAIDs. BioMed Central 2017-04-27 /pmc/articles/PMC5408375/ /pubmed/28449676 http://dx.doi.org/10.1186/s12906-017-1739-7 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chen, Long Liu, Tianjun Wang, Qiangsong Liu, Juan Anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo |
title | Anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo |
title_full | Anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo |
title_fullStr | Anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo |
title_full_unstemmed | Anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo |
title_short | Anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo |
title_sort | anti-inflammatory effect of combined tetramethylpyrazine, resveratrol and curcumin in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408375/ https://www.ncbi.nlm.nih.gov/pubmed/28449676 http://dx.doi.org/10.1186/s12906-017-1739-7 |
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