AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging

Near-IR fluorescence (NIRF) imaging is a new technology using IR fluorescent protein (iRFP) gene labelling and is potentially useful for in vivo applications. In the present study, we expressed iRFP and the TNF-related apoptosis inducing ligand gene in mesenchymal stem cells (MSCs) using adeno-assoc...

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Autores principales: Huang, Can, Lan, Wenjun, Wang, Feifei, Zhang, Chun, Liu, Xiaomei, Chen, Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408655/
https://www.ncbi.nlm.nih.gov/pubmed/28377479
http://dx.doi.org/10.1042/BSR20160556
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author Huang, Can
Lan, Wenjun
Wang, Feifei
Zhang, Chun
Liu, Xiaomei
Chen, Qin
author_facet Huang, Can
Lan, Wenjun
Wang, Feifei
Zhang, Chun
Liu, Xiaomei
Chen, Qin
author_sort Huang, Can
collection PubMed
description Near-IR fluorescence (NIRF) imaging is a new technology using IR fluorescent protein (iRFP) gene labelling and is potentially useful for in vivo applications. In the present study, we expressed iRFP and the TNF-related apoptosis inducing ligand gene in mesenchymal stem cells (MSCs) using adeno-associated virus (AAV) and showed that iRFP-labelled MSCs can be detected by fluorescence microscopy. We injected mice with MSCs labelled with AAV-iRFP, which we were then able to detect by whole-animal NIRF imaging. Our technique provides a visualizable, convenient and sensitive platform for research on tracking the fate of transplanted MSC cells in vivo.
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spelling pubmed-54086552017-05-04 AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging Huang, Can Lan, Wenjun Wang, Feifei Zhang, Chun Liu, Xiaomei Chen, Qin Biosci Rep Research Articles Near-IR fluorescence (NIRF) imaging is a new technology using IR fluorescent protein (iRFP) gene labelling and is potentially useful for in vivo applications. In the present study, we expressed iRFP and the TNF-related apoptosis inducing ligand gene in mesenchymal stem cells (MSCs) using adeno-associated virus (AAV) and showed that iRFP-labelled MSCs can be detected by fluorescence microscopy. We injected mice with MSCs labelled with AAV-iRFP, which we were then able to detect by whole-animal NIRF imaging. Our technique provides a visualizable, convenient and sensitive platform for research on tracking the fate of transplanted MSC cells in vivo. Portland Press Ltd. 2017-04-28 /pmc/articles/PMC5408655/ /pubmed/28377479 http://dx.doi.org/10.1042/BSR20160556 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Huang, Can
Lan, Wenjun
Wang, Feifei
Zhang, Chun
Liu, Xiaomei
Chen, Qin
AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
title AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
title_full AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
title_fullStr AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
title_full_unstemmed AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
title_short AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
title_sort aav-irfp labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408655/
https://www.ncbi.nlm.nih.gov/pubmed/28377479
http://dx.doi.org/10.1042/BSR20160556
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