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A Chinese Variant Marek’s Disease Virus Strain with Divergence between Virulence and Vaccine Resistance

Marek’s disease (MD) virus (MDV) has been evolving continuously, leading to increasing vaccination failure. Here, the MDV field strain BS/15 was isolated from a severely diseased Chinese chicken flock previously vaccinated with CVI988. To explore the causes of vaccination failure, specific-pathogen...

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Autores principales: Sun, Guo-rong, Zhang, Yan-ping, Lv, Hong-chao, Zhou, Lin-yi, Cui, Hong-yu, Gao, Yu-long, Qi, Xiao-le, Wang, Yong-qiang, Li, Kai, Gao, Li, Pan, Qing, Wang, Xiao-mei, Liu, Chang-jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408677/
https://www.ncbi.nlm.nih.gov/pubmed/28368367
http://dx.doi.org/10.3390/v9040071
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author Sun, Guo-rong
Zhang, Yan-ping
Lv, Hong-chao
Zhou, Lin-yi
Cui, Hong-yu
Gao, Yu-long
Qi, Xiao-le
Wang, Yong-qiang
Li, Kai
Gao, Li
Pan, Qing
Wang, Xiao-mei
Liu, Chang-jun
author_facet Sun, Guo-rong
Zhang, Yan-ping
Lv, Hong-chao
Zhou, Lin-yi
Cui, Hong-yu
Gao, Yu-long
Qi, Xiao-le
Wang, Yong-qiang
Li, Kai
Gao, Li
Pan, Qing
Wang, Xiao-mei
Liu, Chang-jun
author_sort Sun, Guo-rong
collection PubMed
description Marek’s disease (MD) virus (MDV) has been evolving continuously, leading to increasing vaccination failure. Here, the MDV field strain BS/15 was isolated from a severely diseased Chinese chicken flock previously vaccinated with CVI988. To explore the causes of vaccination failure, specific-pathogen free (SPF) chickens vaccinated with CVI988 or 814 and unvaccinated controls were challenged with either BS/15 or the reference strain Md5. Both strains induced MD lesions in unvaccinated chickens with similar mortality rates of 85.7% and 80.0% during the experimental period, respectively. However, unvaccinated chickens inoculated with BS/15 exhibited a higher tumor development rate (64.3% vs. 40.0%), but prolonged survival and diminished immune defects compared to Md5-challenged counterparts. These results suggest that BS/15 and Md5 show a similar virulence but manifest with different pathogenic characteristics. Moreover, the protective indices of CVI988 and 814 were 33.3 and 66.7 for BS/15, and 92.9 and 100 for Md5, respectively, indicating that neither vaccine could provide efficient protection against BS/15. Taken together, these data suggest that MD vaccination failure is probably due to the existence of variant MDV strains with known virulence and unexpected vaccine resistance. Our findings should be helpful for understanding the pathogenicity and evolution of MDV strains prevalent in China.
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spelling pubmed-54086772017-05-18 A Chinese Variant Marek’s Disease Virus Strain with Divergence between Virulence and Vaccine Resistance Sun, Guo-rong Zhang, Yan-ping Lv, Hong-chao Zhou, Lin-yi Cui, Hong-yu Gao, Yu-long Qi, Xiao-le Wang, Yong-qiang Li, Kai Gao, Li Pan, Qing Wang, Xiao-mei Liu, Chang-jun Viruses Article Marek’s disease (MD) virus (MDV) has been evolving continuously, leading to increasing vaccination failure. Here, the MDV field strain BS/15 was isolated from a severely diseased Chinese chicken flock previously vaccinated with CVI988. To explore the causes of vaccination failure, specific-pathogen free (SPF) chickens vaccinated with CVI988 or 814 and unvaccinated controls were challenged with either BS/15 or the reference strain Md5. Both strains induced MD lesions in unvaccinated chickens with similar mortality rates of 85.7% and 80.0% during the experimental period, respectively. However, unvaccinated chickens inoculated with BS/15 exhibited a higher tumor development rate (64.3% vs. 40.0%), but prolonged survival and diminished immune defects compared to Md5-challenged counterparts. These results suggest that BS/15 and Md5 show a similar virulence but manifest with different pathogenic characteristics. Moreover, the protective indices of CVI988 and 814 were 33.3 and 66.7 for BS/15, and 92.9 and 100 for Md5, respectively, indicating that neither vaccine could provide efficient protection against BS/15. Taken together, these data suggest that MD vaccination failure is probably due to the existence of variant MDV strains with known virulence and unexpected vaccine resistance. Our findings should be helpful for understanding the pathogenicity and evolution of MDV strains prevalent in China. MDPI 2017-04-03 /pmc/articles/PMC5408677/ /pubmed/28368367 http://dx.doi.org/10.3390/v9040071 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Guo-rong
Zhang, Yan-ping
Lv, Hong-chao
Zhou, Lin-yi
Cui, Hong-yu
Gao, Yu-long
Qi, Xiao-le
Wang, Yong-qiang
Li, Kai
Gao, Li
Pan, Qing
Wang, Xiao-mei
Liu, Chang-jun
A Chinese Variant Marek’s Disease Virus Strain with Divergence between Virulence and Vaccine Resistance
title A Chinese Variant Marek’s Disease Virus Strain with Divergence between Virulence and Vaccine Resistance
title_full A Chinese Variant Marek’s Disease Virus Strain with Divergence between Virulence and Vaccine Resistance
title_fullStr A Chinese Variant Marek’s Disease Virus Strain with Divergence between Virulence and Vaccine Resistance
title_full_unstemmed A Chinese Variant Marek’s Disease Virus Strain with Divergence between Virulence and Vaccine Resistance
title_short A Chinese Variant Marek’s Disease Virus Strain with Divergence between Virulence and Vaccine Resistance
title_sort chinese variant marek’s disease virus strain with divergence between virulence and vaccine resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408677/
https://www.ncbi.nlm.nih.gov/pubmed/28368367
http://dx.doi.org/10.3390/v9040071
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