Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated tha...

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Detalles Bibliográficos
Autores principales: Bilverstone, T.W., Kinsmore, N.L., Minton, N.P., Kuehne, S.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408908/
https://www.ncbi.nlm.nih.gov/pubmed/28108389
http://dx.doi.org/10.1016/j.anaerobe.2017.01.009
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author Bilverstone, T.W.
Kinsmore, N.L.
Minton, N.P.
Kuehne, S.A.
author_facet Bilverstone, T.W.
Kinsmore, N.L.
Minton, N.P.
Kuehne, S.A.
author_sort Bilverstone, T.W.
collection PubMed
description Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.
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spelling pubmed-54089082017-05-05 Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels Bilverstone, T.W. Kinsmore, N.L. Minton, N.P. Kuehne, S.A. Anaerobe Short Communication Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity. Academic Press 2017-04 /pmc/articles/PMC5408908/ /pubmed/28108389 http://dx.doi.org/10.1016/j.anaerobe.2017.01.009 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Short Communication
Bilverstone, T.W.
Kinsmore, N.L.
Minton, N.P.
Kuehne, S.A.
Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels
title Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels
title_full Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels
title_fullStr Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels
title_full_unstemmed Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels
title_short Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels
title_sort development of clostridium difficile r20291δpaloc model strains and in vitro methodologies reveals cdtr is required for the production of cdt to cytotoxic levels
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5408908/
https://www.ncbi.nlm.nih.gov/pubmed/28108389
http://dx.doi.org/10.1016/j.anaerobe.2017.01.009
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