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Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

Illumina’s MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment w...

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Detalles Bibliográficos
Autores principales: Wen, Chongqing, Wu, Liyou, Qin, Yujia, Van Nostrand, Joy D., Ning, Daliang, Sun, Bo, Xue, Kai, Liu, Feifei, Deng, Ye, Liang, Yuting, Zhou, Jizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409056/
https://www.ncbi.nlm.nih.gov/pubmed/28453559
http://dx.doi.org/10.1371/journal.pone.0176716