Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models

New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components–antigens and adjuvants–are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A...

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Autores principales: Tkachuk, A. P., Gushchin, V. A., Potapov, V. D., Demidenko, A. V., Lunin, V. G., Gintsburg, A. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409163/
https://www.ncbi.nlm.nih.gov/pubmed/28453555
http://dx.doi.org/10.1371/journal.pone.0176784
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author Tkachuk, A. P.
Gushchin, V. A.
Potapov, V. D.
Demidenko, A. V.
Lunin, V. G.
Gintsburg, A. L.
author_facet Tkachuk, A. P.
Gushchin, V. A.
Potapov, V. D.
Demidenko, A. V.
Lunin, V. G.
Gintsburg, A. L.
author_sort Tkachuk, A. P.
collection PubMed
description New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components–antigens and adjuvants–are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A and ESAT6-CFP10)–with dextran-binding domain immobilized on dextran and mixed with an adjuvant consisting of DEAE-dextran core, and with CpG oligodeoxynucleotides (TLR9 agonists). GamTBvac and its components were assessed for immunogenicity and protective efficacy in GamTBvac-prime/boost and BCG-prime/ GamTBvac-boost in murine and guinea pig TB models. Results show that in both infectious models, GamTBvac has a strong immunogenicity and significant protective effect against Mycobacterium tuberculosis strain H37Rv under aerosol and intravenous challenges. GamTBvac showed a particularly strong protective effect as a BCG booster vaccine.
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spelling pubmed-54091632017-05-12 Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models Tkachuk, A. P. Gushchin, V. A. Potapov, V. D. Demidenko, A. V. Lunin, V. G. Gintsburg, A. L. PLoS One Research Article New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components–antigens and adjuvants–are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A and ESAT6-CFP10)–with dextran-binding domain immobilized on dextran and mixed with an adjuvant consisting of DEAE-dextran core, and with CpG oligodeoxynucleotides (TLR9 agonists). GamTBvac and its components were assessed for immunogenicity and protective efficacy in GamTBvac-prime/boost and BCG-prime/ GamTBvac-boost in murine and guinea pig TB models. Results show that in both infectious models, GamTBvac has a strong immunogenicity and significant protective effect against Mycobacterium tuberculosis strain H37Rv under aerosol and intravenous challenges. GamTBvac showed a particularly strong protective effect as a BCG booster vaccine. Public Library of Science 2017-04-28 /pmc/articles/PMC5409163/ /pubmed/28453555 http://dx.doi.org/10.1371/journal.pone.0176784 Text en © 2017 Tkachuk et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tkachuk, A. P.
Gushchin, V. A.
Potapov, V. D.
Demidenko, A. V.
Lunin, V. G.
Gintsburg, A. L.
Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models
title Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models
title_full Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models
title_fullStr Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models
title_full_unstemmed Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models
title_short Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models
title_sort multi-subunit bcg booster vaccine gamtbvac: assessment of immunogenicity and protective efficacy in murine and guinea pig tb models
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409163/
https://www.ncbi.nlm.nih.gov/pubmed/28453555
http://dx.doi.org/10.1371/journal.pone.0176784
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