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Effects of Clove Oil and Lidocaine-HCl Anesthesia on Water Parameter during Simulated Transportation in the Marine Medaka, Oryzias dancena

Optimum concentrations of anesthetic clove oil and anesthetic lidocaine-HCl were determined for a species of adult marine medaka, Oryzias dancena, over a range of salinity conditions, and investigated in a transport simulation experiment by analyzing various water and physiological parameters. Resea...

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Autores principales: Park, In-Seok, Gil, Hyun Woo, Lee, Tae Ho, Nam, Yoon Kwon, Lim, Sang Gu, Kim, Dong Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Developmental Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409207/
https://www.ncbi.nlm.nih.gov/pubmed/28484741
http://dx.doi.org/10.12717/DR.2017.21.1.019
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author Park, In-Seok
Gil, Hyun Woo
Lee, Tae Ho
Nam, Yoon Kwon
Lim, Sang Gu
Kim, Dong Soo
author_facet Park, In-Seok
Gil, Hyun Woo
Lee, Tae Ho
Nam, Yoon Kwon
Lim, Sang Gu
Kim, Dong Soo
author_sort Park, In-Seok
collection PubMed
description Optimum concentrations of anesthetic clove oil and anesthetic lidocaine-HCl were determined for a species of adult marine medaka, Oryzias dancena, over a range of salinity conditions, and investigated in a transport simulation experiment by analyzing various water and physiological parameters. Research indicated that the higher the concentration of anesthetic at each salinity, the shorter the anesthesia time at each salinity. At each concentration, fish were anesthetized slower at water salinities over 10 ppt (P<0.05). Anesthesia time at 10 ppt was faster than any other salinity. In 10 ppt salinity, the dissolved oxygen (DO) concentrations and respiratory frequencies of the clove-oil-administered groups decreased until 48 hours (P<0.05), whereas the NH(4)(+) and CO(2) concentrations increased until 48 hours (P<0.05). In same period, the DO, NH(4)(+), and CO(2) concentrations and respiratory frequencies all decreased as the clove oil concentration increased (P<0.05). The trends in the DO, NH(4)(+), and CO(2) concentrations and respiratory frequencies in the lidocaine-HCl-administered groups were similar to those in the clove-oil-administered groups. In conclusion, clove oil and lidocaine-HCl are effective anesthetics, improving the transportation of the marine medaka. The results from this study will contribute to safe laboratory handling of the marine medaka, which are commonly required by many research studies and experiments.
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spelling pubmed-54092072017-05-08 Effects of Clove Oil and Lidocaine-HCl Anesthesia on Water Parameter during Simulated Transportation in the Marine Medaka, Oryzias dancena Park, In-Seok Gil, Hyun Woo Lee, Tae Ho Nam, Yoon Kwon Lim, Sang Gu Kim, Dong Soo Dev Reprod Original Research Paper Optimum concentrations of anesthetic clove oil and anesthetic lidocaine-HCl were determined for a species of adult marine medaka, Oryzias dancena, over a range of salinity conditions, and investigated in a transport simulation experiment by analyzing various water and physiological parameters. Research indicated that the higher the concentration of anesthetic at each salinity, the shorter the anesthesia time at each salinity. At each concentration, fish were anesthetized slower at water salinities over 10 ppt (P<0.05). Anesthesia time at 10 ppt was faster than any other salinity. In 10 ppt salinity, the dissolved oxygen (DO) concentrations and respiratory frequencies of the clove-oil-administered groups decreased until 48 hours (P<0.05), whereas the NH(4)(+) and CO(2) concentrations increased until 48 hours (P<0.05). In same period, the DO, NH(4)(+), and CO(2) concentrations and respiratory frequencies all decreased as the clove oil concentration increased (P<0.05). The trends in the DO, NH(4)(+), and CO(2) concentrations and respiratory frequencies in the lidocaine-HCl-administered groups were similar to those in the clove-oil-administered groups. In conclusion, clove oil and lidocaine-HCl are effective anesthetics, improving the transportation of the marine medaka. The results from this study will contribute to safe laboratory handling of the marine medaka, which are commonly required by many research studies and experiments. The Korean Society of Developmental Biology 2017-03 2017-03-31 /pmc/articles/PMC5409207/ /pubmed/28484741 http://dx.doi.org/10.12717/DR.2017.21.1.019 Text en ⓒ Copyright an Official Journal of the Korean Society of Developmental Biology. All Rights Reserved http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Paper
Park, In-Seok
Gil, Hyun Woo
Lee, Tae Ho
Nam, Yoon Kwon
Lim, Sang Gu
Kim, Dong Soo
Effects of Clove Oil and Lidocaine-HCl Anesthesia on Water Parameter during Simulated Transportation in the Marine Medaka, Oryzias dancena
title Effects of Clove Oil and Lidocaine-HCl Anesthesia on Water Parameter during Simulated Transportation in the Marine Medaka, Oryzias dancena
title_full Effects of Clove Oil and Lidocaine-HCl Anesthesia on Water Parameter during Simulated Transportation in the Marine Medaka, Oryzias dancena
title_fullStr Effects of Clove Oil and Lidocaine-HCl Anesthesia on Water Parameter during Simulated Transportation in the Marine Medaka, Oryzias dancena
title_full_unstemmed Effects of Clove Oil and Lidocaine-HCl Anesthesia on Water Parameter during Simulated Transportation in the Marine Medaka, Oryzias dancena
title_short Effects of Clove Oil and Lidocaine-HCl Anesthesia on Water Parameter during Simulated Transportation in the Marine Medaka, Oryzias dancena
title_sort effects of clove oil and lidocaine-hcl anesthesia on water parameter during simulated transportation in the marine medaka, oryzias dancena
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409207/
https://www.ncbi.nlm.nih.gov/pubmed/28484741
http://dx.doi.org/10.12717/DR.2017.21.1.019
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