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Modification of fatty acid selectivity of Candida antarctica lipase A by error-prone PCR

OBJECTIVE: To generate Candida antarctica lipase A (CAL-A) mutants with modified fatty acid selectivities and improved lipolytic activities using error-prone PCR (epPCR). RESULTS: A Candida antarctica lipase A mutant was obtained in three rounds of epPCR. This mutant showed a 14 times higher ability...

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Detalles Bibliográficos
Autor principal: Głód, Dagmara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409807/
https://www.ncbi.nlm.nih.gov/pubmed/28281023
http://dx.doi.org/10.1007/s10529-017-2299-0