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Regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression

The incidence of glioma in men is higher than that in women; however, little is known about the expression and basic function of the androgen receptor (AR) in gliomas. AR inhibited the small VCP/p97-interacting protein (SVIP) on the transcriptional level was previously reported. The present study sh...

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Autores principales: Bao, Dejun, Cheng, Chuandong, Lan, Xiaoqiang, Xing, Rong, Chen, Zhuo, Zhao, Hua, Sun, Junyan, Wang, Yang, Niu, Chaoshi, Zhang, Bo, Fang, Shengyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5410292/
https://www.ncbi.nlm.nih.gov/pubmed/28423563
http://dx.doi.org/10.18632/oncotarget.15509
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author Bao, Dejun
Cheng, Chuandong
Lan, Xiaoqiang
Xing, Rong
Chen, Zhuo
Zhao, Hua
Sun, Junyan
Wang, Yang
Niu, Chaoshi
Zhang, Bo
Fang, Shengyun
author_facet Bao, Dejun
Cheng, Chuandong
Lan, Xiaoqiang
Xing, Rong
Chen, Zhuo
Zhao, Hua
Sun, Junyan
Wang, Yang
Niu, Chaoshi
Zhang, Bo
Fang, Shengyun
author_sort Bao, Dejun
collection PubMed
description The incidence of glioma in men is higher than that in women; however, little is known about the expression and basic function of the androgen receptor (AR) in gliomas. AR inhibited the small VCP/p97-interacting protein (SVIP) on the transcriptional level was previously reported. The present study shows that the protein level of AR is highly expressed in cell lines of the nervous system. Moreover, the AR expression is increased while SVIP expression is decreased in tumor tissue of glioma patients, which is in agreement with the progressing WHO grades. A statistically significant increase in serum testosterone level of glioma patients compared with that of non-cancer patients was also detected. Furthermore, it has been proved that SVIP is down-regulated as well as AR is up-regulated in glioma cell lines with R1881 treatment. Interestingly, the depletion of SVIP using siRNA facilitated cell proliferation and decreased p53 expression. In addition, overexpression of SVIP increased cell death only in p53(wt) cell lines. Moreover, U87MG cells, p53(wt) cell line was susceptible to AR antagonists in vitro and in vivo. The current study provides insight into the biological role of AR in suppressing SVIP and p53 and promoting the progression of glioma as well as the clinical treatment of glioma patients.
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spelling pubmed-54102922017-05-04 Regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression Bao, Dejun Cheng, Chuandong Lan, Xiaoqiang Xing, Rong Chen, Zhuo Zhao, Hua Sun, Junyan Wang, Yang Niu, Chaoshi Zhang, Bo Fang, Shengyun Oncotarget Research Paper The incidence of glioma in men is higher than that in women; however, little is known about the expression and basic function of the androgen receptor (AR) in gliomas. AR inhibited the small VCP/p97-interacting protein (SVIP) on the transcriptional level was previously reported. The present study shows that the protein level of AR is highly expressed in cell lines of the nervous system. Moreover, the AR expression is increased while SVIP expression is decreased in tumor tissue of glioma patients, which is in agreement with the progressing WHO grades. A statistically significant increase in serum testosterone level of glioma patients compared with that of non-cancer patients was also detected. Furthermore, it has been proved that SVIP is down-regulated as well as AR is up-regulated in glioma cell lines with R1881 treatment. Interestingly, the depletion of SVIP using siRNA facilitated cell proliferation and decreased p53 expression. In addition, overexpression of SVIP increased cell death only in p53(wt) cell lines. Moreover, U87MG cells, p53(wt) cell line was susceptible to AR antagonists in vitro and in vivo. The current study provides insight into the biological role of AR in suppressing SVIP and p53 and promoting the progression of glioma as well as the clinical treatment of glioma patients. Impact Journals LLC 2017-02-19 /pmc/articles/PMC5410292/ /pubmed/28423563 http://dx.doi.org/10.18632/oncotarget.15509 Text en Copyright: © 2017 Bao et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Bao, Dejun
Cheng, Chuandong
Lan, Xiaoqiang
Xing, Rong
Chen, Zhuo
Zhao, Hua
Sun, Junyan
Wang, Yang
Niu, Chaoshi
Zhang, Bo
Fang, Shengyun
Regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression
title Regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression
title_full Regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression
title_fullStr Regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression
title_full_unstemmed Regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression
title_short Regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small VCP/p97-interacting protein expression
title_sort regulation of p53(wt) glioma cell proliferation by androgen receptor-mediated inhibition of small vcp/p97-interacting protein expression
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5410292/
https://www.ncbi.nlm.nih.gov/pubmed/28423563
http://dx.doi.org/10.18632/oncotarget.15509
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