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The protection of meloxicam against chronic aluminium overload-induced liver injury in rats
The present study was designed to observe the protective effect and mechanisms of meloxicam on liver injury caused by chronic aluminium exposure in rats. The histopathology was detected by hematoxylin-eosin staining. The levels of prostaglandin E2, cyclic adenosine monophosphate and inflammatory cyt...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5410317/ https://www.ncbi.nlm.nih.gov/pubmed/28423583 http://dx.doi.org/10.18632/oncotarget.15588 |
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author | Yang, Yang He, Qin Wang, Hong Hu, Xinyue Luo, Ying Liang, Guojuan Kuang, Shengnan Mai, Shaoshan Ma, Jie Tian, Xiaoyan Chen, Qi Yang, Junqing |
author_facet | Yang, Yang He, Qin Wang, Hong Hu, Xinyue Luo, Ying Liang, Guojuan Kuang, Shengnan Mai, Shaoshan Ma, Jie Tian, Xiaoyan Chen, Qi Yang, Junqing |
author_sort | Yang, Yang |
collection | PubMed |
description | The present study was designed to observe the protective effect and mechanisms of meloxicam on liver injury caused by chronic aluminium exposure in rats. The histopathology was detected by hematoxylin-eosin staining. The levels of prostaglandin E2, cyclic adenosine monophosphate and inflammatory cytokines were detected by enzyme linked immunosorbent assay. The expressions of cyclooxygenases-2, prostaglandin E2 receptors and protein kinase A were measured by western blotting and immunohistochemistry. Our experimental results showed that aluminium overload significantly damaged the liver. Aluminium also significantly increased the expressions of cyclooxygenases-2, prostaglandin E2, cyclic adenosine monophosphate, protein kinase A and the prostaglandin E2 receptors (EP(1,2,4)) and the levels of inflammation and oxidative stress, while significantly decreased the EP3 expression in liver. The administration of meloxicam significantly improved the impairment of liver. The contents of prostaglandin E2 and cyclic adenosine monophosphate were significantly decreased by administration of meloxicam. The administration of meloxicam also significantly decreased the expressions of cyclooxygenases-2 and protein kinase A and the levels of inflammation and oxidative stress, while significantly increased the EP(1,2,3,4) expressions in rat liver. Our results suggested that the imbalance of cyclooxygenases-2 and downstream prostaglandin E2 signaling pathway is involved in the injury of chronic aluminium-overload rat liver. The protective mechanism of meloxicam on aluminium-overload liver injury is attributed to reconstruct the balance of cyclooxygenases-2 and downstream prostaglandin E2 signaling pathway. |
format | Online Article Text |
id | pubmed-5410317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-54103172017-05-04 The protection of meloxicam against chronic aluminium overload-induced liver injury in rats Yang, Yang He, Qin Wang, Hong Hu, Xinyue Luo, Ying Liang, Guojuan Kuang, Shengnan Mai, Shaoshan Ma, Jie Tian, Xiaoyan Chen, Qi Yang, Junqing Oncotarget Research Paper The present study was designed to observe the protective effect and mechanisms of meloxicam on liver injury caused by chronic aluminium exposure in rats. The histopathology was detected by hematoxylin-eosin staining. The levels of prostaglandin E2, cyclic adenosine monophosphate and inflammatory cytokines were detected by enzyme linked immunosorbent assay. The expressions of cyclooxygenases-2, prostaglandin E2 receptors and protein kinase A were measured by western blotting and immunohistochemistry. Our experimental results showed that aluminium overload significantly damaged the liver. Aluminium also significantly increased the expressions of cyclooxygenases-2, prostaglandin E2, cyclic adenosine monophosphate, protein kinase A and the prostaglandin E2 receptors (EP(1,2,4)) and the levels of inflammation and oxidative stress, while significantly decreased the EP3 expression in liver. The administration of meloxicam significantly improved the impairment of liver. The contents of prostaglandin E2 and cyclic adenosine monophosphate were significantly decreased by administration of meloxicam. The administration of meloxicam also significantly decreased the expressions of cyclooxygenases-2 and protein kinase A and the levels of inflammation and oxidative stress, while significantly increased the EP(1,2,3,4) expressions in rat liver. Our results suggested that the imbalance of cyclooxygenases-2 and downstream prostaglandin E2 signaling pathway is involved in the injury of chronic aluminium-overload rat liver. The protective mechanism of meloxicam on aluminium-overload liver injury is attributed to reconstruct the balance of cyclooxygenases-2 and downstream prostaglandin E2 signaling pathway. Impact Journals LLC 2017-02-21 /pmc/articles/PMC5410317/ /pubmed/28423583 http://dx.doi.org/10.18632/oncotarget.15588 Text en Copyright: © 2017 Yang et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Yang, Yang He, Qin Wang, Hong Hu, Xinyue Luo, Ying Liang, Guojuan Kuang, Shengnan Mai, Shaoshan Ma, Jie Tian, Xiaoyan Chen, Qi Yang, Junqing The protection of meloxicam against chronic aluminium overload-induced liver injury in rats |
title | The protection of meloxicam against chronic aluminium overload-induced liver injury in rats |
title_full | The protection of meloxicam against chronic aluminium overload-induced liver injury in rats |
title_fullStr | The protection of meloxicam against chronic aluminium overload-induced liver injury in rats |
title_full_unstemmed | The protection of meloxicam against chronic aluminium overload-induced liver injury in rats |
title_short | The protection of meloxicam against chronic aluminium overload-induced liver injury in rats |
title_sort | protection of meloxicam against chronic aluminium overload-induced liver injury in rats |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5410317/ https://www.ncbi.nlm.nih.gov/pubmed/28423583 http://dx.doi.org/10.18632/oncotarget.15588 |
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