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A method for labeling proteins with tags at the native genomic loci in budding yeast

Fluorescent proteins and epitope tags are often used as protein fusion tags to study target proteins. One prevailing technique in the budding yeast Saccharomyces cerevisiae is to fuse these tags to a target gene at the precise chromosomal location via homologous recombination. However, several limit...

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Detalles Bibliográficos
Autores principales:	Wang, Qian, Xue, Huijun, Li, Siqi, Chen, Ying, Tian, Xuelei, Xu, Xin, Xiao, Wei, Fu, Yu Vincent
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411076/ https://www.ncbi.nlm.nih.gov/pubmed/28459859 http://dx.doi.org/10.1371/journal.pone.0176184

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