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An improved Red/ET recombineering system and mouse ES cells culture conditions for the generation of targeted mutant mice
Targeted mutant mice generated on a C57BL/6 background are powerful tools for analysis of the biological functions of genes, and gene targeting technologies using mouse embryonic stem (ES) cells have been used to generate such mice. Recently, a bacterial artificial chromosome (BAC) recombineering sy...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411299/ https://www.ncbi.nlm.nih.gov/pubmed/27890869 http://dx.doi.org/10.1538/expanim.16-0075 |