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An improved Red/ET recombineering system and mouse ES cells culture conditions for the generation of targeted mutant mice

Targeted mutant mice generated on a C57BL/6 background are powerful tools for analysis of the biological functions of genes, and gene targeting technologies using mouse embryonic stem (ES) cells have been used to generate such mice. Recently, a bacterial artificial chromosome (BAC) recombineering sy...

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Detalles Bibliográficos
Autores principales:	Kumagai, Katsuyoshi, Takanashi, Masakatsu, Ohno, Shin-ichiro, Kuroda, Masahiko, Sudo, Katsuko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Japanese Association for Laboratory Animal Science 2016
Materias:	Original
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411299/ https://www.ncbi.nlm.nih.gov/pubmed/27890869 http://dx.doi.org/10.1538/expanim.16-0075

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