Cargando…

An improved Red/ET recombineering system and mouse ES cells culture conditions for the generation of targeted mutant mice

Targeted mutant mice generated on a C57BL/6 background are powerful tools for analysis of the biological functions of genes, and gene targeting technologies using mouse embryonic stem (ES) cells have been used to generate such mice. Recently, a bacterial artificial chromosome (BAC) recombineering sy...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kumagai, Katsuyoshi, Takanashi, Masakatsu, Ohno, Shin-ichiro, Kuroda, Masahiko, Sudo, Katsuko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Japanese Association for Laboratory Animal Science 2016
Materias:	Original
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411299/ https://www.ncbi.nlm.nih.gov/pubmed/27890869 http://dx.doi.org/10.1538/expanim.16-0075

Ejemplares similares

Extracellular microvesicles that originated adipose tissue derived mesenchymal stem cells have the potential ability to improve rheumatoid arthritis on mice
por: Tsujimaru, Koichiro, et al.
Publicado: (2020)

Novel form of miR-29b suppresses bleomycin-induced pulmonary fibrosis
por: Yamada, Yuko, et al.
Publicado: (2017)

WAPL induces cervical intraepithelial neoplasia modulated with estrogen signaling without HPV E6/E7
por: Kumagai, Katsuyoshi, et al.
Publicado: (2021)

Acerola exosome-like nanovesicles to systemically deliver nucleic acid medicine via oral administration
por: Umezu, Tomohiro, et al.
Publicado: (2021)

Novel Types of Small RNA Exhibit Sequence- and Target-dependent Angiogenesis Suppression Without Activation of Toll-like Receptor 3 in an Age-related Macular Degeneration (AMD) Mouse Model
por: Takanashi, Masakatsu, et al.
Publicado: (2015)

Cisplatin induces differentiation in teratomas derived from pluripotent stem cells
por: Kurata, Atsushi, et al.
Publicado: (2021)

Expression level of microRNA-200c is associated with cell morphology in vitro and histological differentiation through regulation of ZEB1/2 and E-cadherin in gastric carcinoma
por: Kurata, Atsushi, et al.
Publicado: (2018)

Distinctive Tissue and Serum MicroRNA Profile of IgG4-Related Ophthalmic Disease and MALT Lymphoma
por: Nezu, Naoya, et al.
Publicado: (2020)

Down-Regulation of miR-92 in Human Plasma Is a Novel Marker for Acute Leukemia Patients
por: Tanaka, Masami, et al.
Publicado: (2009)

Anthocyanidins-enriched bilberry extracts inhibit 3T3-L1 adipocyte differentiation via the insulin pathway
por: Suzuki, Rieko, et al.
Publicado: (2011)

IL-1 Plays an Important Role in Lipid Metabolism by Regulating Insulin Levels under Physiological Conditions
por: Matsuki, Taizo, et al.
Publicado: (2003)

Sigrid et es mondes perdus /
por: Brussolo, Serge, 1951-
Publicado: (2006)

Tregua ; La red ; Geografía es amor /
por: García Nieto, José
Publicado: (1982)

Generation of a mouse mutant by oligonucleotide-mediated gene modification in ES cells
por: Aarts, Marieke, et al.
Publicado: (2006)

Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State
por: Devika, A. S., et al.
Publicado: (2021)

Comprehensive miRNA Analysis Using Serum From Patients With Noninfectious Uveitis
por: Asakage, Masaki, et al.
Publicado: (2020)

Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(®)/ET(® )Recombination
por: Heermann, Ralf, et al.
Publicado: (2008)

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)—an alternative and enhanced method for large fragment recombineering
por: Rivero-Müller, Adolfo, et al.
Publicado: (2007)

¿Qué es la historia cultural? /
por: Burke, Peter, 1937-
Publicado: (2006)

Age-dependent sex difference of non-alcoholic fatty liver disease in TSOD and db/db mice
por: Dungubat, Erdenetsogt, et al.
Publicado: (2022)

Medaka Cleavage Embryos Are Capable of Generating ES-Like Cell Cultures
por: Li, Zhendong, et al.
Publicado: (2011)

Focus on Extracellular Vesicles: Development of Extracellular Vesicle-Based Therapeutic Systems
por: Ohno, Shin-ichiro, et al.
Publicado: (2016)

Efficient generation of selection‐gene‐free rat knockout models by homologous recombination in ES cells
por: Lan, He, et al.
Publicado: (2016)

Molecular Characterization of Mutant Mouse Strains Generated from the EUCOMM/KOMP-CSD ES Cell Resource
por: Ryder, Edward, et al.
Publicado: (2013)

Genetic engineering and heterologous expression of the disorazol biosynthetic gene cluster via Red/ET recombineering
por: Tu, Qiang, et al.
Publicado: (2016)

Cyclic GMP signalling in human ES and iPS-derived vascular cells
por: Taura, Daisuke, et al.
Publicado: (2009)

La science et la religion r ©concili ©es
Publicado: (2009)

Inhibitory and inductive effects of 4- or 5-methyl-2-mercaptobenzimidazole, thyrotoxic and hepatotoxic rubber antioxidants, on several forms of cytochrome P450 in primary cultured rat and human hepatocytes
por: Miyajima, Atsuko, et al.
Publicado: (2020)

Christe, qui lux es et dies = O Christ, who art the light and day
por: Byrd, William, 1542 or 3-1623
Publicado: (1966)

High-Throughput MicroRNA Profiling of Vitreoretinal Lymphoma: Vitreous and Serum MicroRNA Profiles Distinct from Uveitis
por: Minezaki, Teruumi, et al.
Publicado: (2020)

p53 Gene Targeting by Homologous Recombination in Fish ES Cells
por: Yan, Yan, et al.
Publicado: (2013)

Expression of L-type amino acid transporter 1 is a poor prognostic factor for Non-Hodgkin’s lymphoma
por: Jigjidkhorloo, Narangerel, et al.
Publicado: (2021)

Effects of Sex, Age, and Fasting Conditions on Plasma Lipidomic Profiles of Fasted Sprague-Dawley Rats
por: Saito, Kosuke, et al.
Publicado: (2014)

A case report on death from acute bacterial cholangitis accompanied by von Meyenburg complexes: Use of 16S rRNA gene sequencing to identify pathogenic microbes from postmortem formalin-fixed, paraffin-embedded tissue
por: Watanabe, Noriko, et al.
Publicado: (2021)

In vitro culture at 39 °C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions
por: Imamura, Satoshi, et al.
Publicado: (2022)

Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa
por: Lesic, Biliana, et al.
Publicado: (2008)

Whole-Genome Analysis of Herbicide-Tolerant Mutant Rice Generated by Agrobacterium-Mediated Gene Targeting
por: Endo, Masaki, et al.
Publicado: (2015)

Whole-Transcriptome Analysis Reveals Autophagy Is Involved in Early Senescence of zj-es Mutant Rice
por: Sun, Jia, et al.
Publicado: (2022)

¿Es amor o es adicción?
por: Schaeffer, Brenda
Publicado: (1992)

Leer es respirar, es devenir /
por: Ólafur Elíasson, 1967-
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_fkkmlj5fg2pr4m9dbp83mdf394