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Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts

Aquatic chytrid fungi threaten amphibian biodiversity worldwide owing to their ability to rapidly expand their geographical distributions and to infect a wide range of hosts. Combating this risk requires an understanding of chytrid host range to identify potential reservoirs of infection and to safe...

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Autores principales: Liew, Nicole, Mazon Moya, Maria J., Wierzbicki, Claudia J., Hollinshead, Michael, Dillon, Michael J., Thornton, Christopher R., Ellison, Amy, Cable, Jo, Fisher, Matthew C., Mostowy, Serge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411484/
https://www.ncbi.nlm.nih.gov/pubmed/28425465
http://dx.doi.org/10.1038/ncomms15048
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author Liew, Nicole
Mazon Moya, Maria J.
Wierzbicki, Claudia J.
Hollinshead, Michael
Dillon, Michael J.
Thornton, Christopher R.
Ellison, Amy
Cable, Jo
Fisher, Matthew C.
Mostowy, Serge
author_facet Liew, Nicole
Mazon Moya, Maria J.
Wierzbicki, Claudia J.
Hollinshead, Michael
Dillon, Michael J.
Thornton, Christopher R.
Ellison, Amy
Cable, Jo
Fisher, Matthew C.
Mostowy, Serge
author_sort Liew, Nicole
collection PubMed
description Aquatic chytrid fungi threaten amphibian biodiversity worldwide owing to their ability to rapidly expand their geographical distributions and to infect a wide range of hosts. Combating this risk requires an understanding of chytrid host range to identify potential reservoirs of infection and to safeguard uninfected regions through enhanced biosecurity. Here we extend our knowledge on the host range of the chytrid Batrachochytrium dendrobatidis by demonstrating infection of a non-amphibian vertebrate host, the zebrafish. We observe dose-dependent mortality and show that chytrid can infect and proliferate on zebrafish tissue. We also show that infection phenotypes (fin erosion, cell apoptosis and muscle degeneration) are direct symptoms of infection. Successful infection is dependent on disrupting the zebrafish microbiome, highlighting that, as is widely found in amphibians, commensal bacteria confer protection against this pathogen. Collectively, our findings greatly expand the limited tool kit available to study pathogenesis and host response to chytrid infection.
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spelling pubmed-54114842017-07-11 Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts Liew, Nicole Mazon Moya, Maria J. Wierzbicki, Claudia J. Hollinshead, Michael Dillon, Michael J. Thornton, Christopher R. Ellison, Amy Cable, Jo Fisher, Matthew C. Mostowy, Serge Nat Commun Article Aquatic chytrid fungi threaten amphibian biodiversity worldwide owing to their ability to rapidly expand their geographical distributions and to infect a wide range of hosts. Combating this risk requires an understanding of chytrid host range to identify potential reservoirs of infection and to safeguard uninfected regions through enhanced biosecurity. Here we extend our knowledge on the host range of the chytrid Batrachochytrium dendrobatidis by demonstrating infection of a non-amphibian vertebrate host, the zebrafish. We observe dose-dependent mortality and show that chytrid can infect and proliferate on zebrafish tissue. We also show that infection phenotypes (fin erosion, cell apoptosis and muscle degeneration) are direct symptoms of infection. Successful infection is dependent on disrupting the zebrafish microbiome, highlighting that, as is widely found in amphibians, commensal bacteria confer protection against this pathogen. Collectively, our findings greatly expand the limited tool kit available to study pathogenesis and host response to chytrid infection. Nature Publishing Group 2017-04-20 /pmc/articles/PMC5411484/ /pubmed/28425465 http://dx.doi.org/10.1038/ncomms15048 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liew, Nicole
Mazon Moya, Maria J.
Wierzbicki, Claudia J.
Hollinshead, Michael
Dillon, Michael J.
Thornton, Christopher R.
Ellison, Amy
Cable, Jo
Fisher, Matthew C.
Mostowy, Serge
Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts
title Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts
title_full Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts
title_fullStr Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts
title_full_unstemmed Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts
title_short Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts
title_sort chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411484/
https://www.ncbi.nlm.nih.gov/pubmed/28425465
http://dx.doi.org/10.1038/ncomms15048
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