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Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation

B-type lamins are major constituents of the nuclear lamina in all metazoan cells, yet have specific roles in the development of certain cell types. Although they are speculated to regulate gene expression in developmental contexts, a direct link between B-type lamins and developmental gene expressio...

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Autores principales: Gigante, Crystal M., Dibattista, Michele, Dong, Frederick N., Zheng, Xiaobin, Yue, Sibiao, Young, Stephen G., Reisert, Johannes, Zheng, Yixian, Zhao, Haiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411488/
https://www.ncbi.nlm.nih.gov/pubmed/28425486
http://dx.doi.org/10.1038/ncomms15098
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author Gigante, Crystal M.
Dibattista, Michele
Dong, Frederick N.
Zheng, Xiaobin
Yue, Sibiao
Young, Stephen G.
Reisert, Johannes
Zheng, Yixian
Zhao, Haiqing
author_facet Gigante, Crystal M.
Dibattista, Michele
Dong, Frederick N.
Zheng, Xiaobin
Yue, Sibiao
Young, Stephen G.
Reisert, Johannes
Zheng, Yixian
Zhao, Haiqing
author_sort Gigante, Crystal M.
collection PubMed
description B-type lamins are major constituents of the nuclear lamina in all metazoan cells, yet have specific roles in the development of certain cell types. Although they are speculated to regulate gene expression in developmental contexts, a direct link between B-type lamins and developmental gene expression in an in vivo system is currently lacking. Here, we identify lamin B1 as a key regulator of gene expression required for the formation of functional olfactory sensory neurons. By using targeted knockout in olfactory epithelial stem cells in adult mice, we show that lamin B1 deficient neurons exhibit attenuated response to odour stimulation. This deficit can be explained by decreased expression of genes involved in mature neuron function, along with increased expression of genes atypical of the olfactory lineage. These results support that the broadly expressed lamin B1 regulates expression of a subset of genes involved in the differentiation of a specific cell type.
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spelling pubmed-54114882017-07-11 Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation Gigante, Crystal M. Dibattista, Michele Dong, Frederick N. Zheng, Xiaobin Yue, Sibiao Young, Stephen G. Reisert, Johannes Zheng, Yixian Zhao, Haiqing Nat Commun Article B-type lamins are major constituents of the nuclear lamina in all metazoan cells, yet have specific roles in the development of certain cell types. Although they are speculated to regulate gene expression in developmental contexts, a direct link between B-type lamins and developmental gene expression in an in vivo system is currently lacking. Here, we identify lamin B1 as a key regulator of gene expression required for the formation of functional olfactory sensory neurons. By using targeted knockout in olfactory epithelial stem cells in adult mice, we show that lamin B1 deficient neurons exhibit attenuated response to odour stimulation. This deficit can be explained by decreased expression of genes involved in mature neuron function, along with increased expression of genes atypical of the olfactory lineage. These results support that the broadly expressed lamin B1 regulates expression of a subset of genes involved in the differentiation of a specific cell type. Nature Publishing Group 2017-04-20 /pmc/articles/PMC5411488/ /pubmed/28425486 http://dx.doi.org/10.1038/ncomms15098 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Gigante, Crystal M.
Dibattista, Michele
Dong, Frederick N.
Zheng, Xiaobin
Yue, Sibiao
Young, Stephen G.
Reisert, Johannes
Zheng, Yixian
Zhao, Haiqing
Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation
title Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation
title_full Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation
title_fullStr Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation
title_full_unstemmed Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation
title_short Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation
title_sort lamin b1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411488/
https://www.ncbi.nlm.nih.gov/pubmed/28425486
http://dx.doi.org/10.1038/ncomms15098
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