Cargando…
BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATIONS OF EXTRACTS FROM PTEROCARPUS ERINACEUS POIR (FABACEAE) ROOT BARKS
BACKGROUND: Pterocarpus erinaceus Poir. belonging to Fabacae familly is used as medicinal plant in Burkina Faso’s folk medicine. Roots of P. erinaceus are used to treat ulcer, stomach ache and inflammatory diseases. The objective of the present study was to carry out phytochemical composition of met...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
African Traditional Herbal Medicine Supporters Initiative (ATHMSI)
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411871/ https://www.ncbi.nlm.nih.gov/pubmed/28480397 http://dx.doi.org/10.21010/ajtcam.v14i1.21 |
_version_ | 1783232880955097088 |
---|---|
author | Noufou, Ouédraogo Anne-Emmanuelle, Hay Claude W, Ouédraogo Jean Richard, Sawadogo W André, Tibiri Marius, Lompo Jean-baptiste, Nikiema Jean, Koudou Marie-Genevieve, Dijoux-Franca Pierre, Guissou Innocent |
author_facet | Noufou, Ouédraogo Anne-Emmanuelle, Hay Claude W, Ouédraogo Jean Richard, Sawadogo W André, Tibiri Marius, Lompo Jean-baptiste, Nikiema Jean, Koudou Marie-Genevieve, Dijoux-Franca Pierre, Guissou Innocent |
author_sort | Noufou, Ouédraogo |
collection | PubMed |
description | BACKGROUND: Pterocarpus erinaceus Poir. belonging to Fabacae familly is used as medicinal plant in Burkina Faso’s folk medicine. Roots of P. erinaceus are used to treat ulcer, stomach ache and inflammatory diseases. The objective of the present study was to carry out phytochemical composition of methanol (MeOH) and dichloromethane (DCM) extracts from Pterocarpus erinaceus roots, to isolate pure compounds, and to evaluate their pharmacological activities. METHODS: Chromatographic fractionation led to the isolation of active components of the extracts. The structures were established by NMR analysis and comparison with data from literature. The anti-inflammatory activity was evaluated using croton oil-induced edema of mice ear as well as the effect of extracts against lipoxygenase and lipid peroxidation was evaluated. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Cupric-reducing antioxidant capacity (CUPRAC) methods were used to evaluate the antioxidant activity of the extracts. RESULTS: Friedelin (1), 3a-hydroxyfriedelan-2-one (2), a-sophoradiol (3) and stigmasterol (4) were isolated from DCM extract and maltol-6-O-apiofuranoside-glucopyranoside (5) isolated from MeOH. DCM extract and friedelin, 3a-hydroxyfriedelan-2-one, a-sophoradiol showed a significant anti-inflammatory effect against ear edema. Friedelin (1), α-sophoradiol (3) and maltol-6-O-apiofuranoside-glucopyranoside (5) exhibited lipoxygenase inhibition. MeOH extract (100 μg/mL) inhibited lipoxygenase and lipid peroxidation activities at 45.1 ± 3% and 30.7 ± 0.5% respectively. MeOH extract, ethyl acetate fraction and butanol fraction exhibited antioxidant property with both two methods used. CONCLUSION: The results suggested that the extracts and compounds from roots of Pterocarpus erinaceus possessed local anti-inflammatory effect, antioxidant properties and inhibitor effect against lipoxygenase and lipid peroxidation activities. |
format | Online Article Text |
id | pubmed-5411871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-54118712017-06-05 BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATIONS OF EXTRACTS FROM PTEROCARPUS ERINACEUS POIR (FABACEAE) ROOT BARKS Noufou, Ouédraogo Anne-Emmanuelle, Hay Claude W, Ouédraogo Jean Richard, Sawadogo W André, Tibiri Marius, Lompo Jean-baptiste, Nikiema Jean, Koudou Marie-Genevieve, Dijoux-Franca Pierre, Guissou Innocent Afr J Tradit Complement Altern Med Article BACKGROUND: Pterocarpus erinaceus Poir. belonging to Fabacae familly is used as medicinal plant in Burkina Faso’s folk medicine. Roots of P. erinaceus are used to treat ulcer, stomach ache and inflammatory diseases. The objective of the present study was to carry out phytochemical composition of methanol (MeOH) and dichloromethane (DCM) extracts from Pterocarpus erinaceus roots, to isolate pure compounds, and to evaluate their pharmacological activities. METHODS: Chromatographic fractionation led to the isolation of active components of the extracts. The structures were established by NMR analysis and comparison with data from literature. The anti-inflammatory activity was evaluated using croton oil-induced edema of mice ear as well as the effect of extracts against lipoxygenase and lipid peroxidation was evaluated. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Cupric-reducing antioxidant capacity (CUPRAC) methods were used to evaluate the antioxidant activity of the extracts. RESULTS: Friedelin (1), 3a-hydroxyfriedelan-2-one (2), a-sophoradiol (3) and stigmasterol (4) were isolated from DCM extract and maltol-6-O-apiofuranoside-glucopyranoside (5) isolated from MeOH. DCM extract and friedelin, 3a-hydroxyfriedelan-2-one, a-sophoradiol showed a significant anti-inflammatory effect against ear edema. Friedelin (1), α-sophoradiol (3) and maltol-6-O-apiofuranoside-glucopyranoside (5) exhibited lipoxygenase inhibition. MeOH extract (100 μg/mL) inhibited lipoxygenase and lipid peroxidation activities at 45.1 ± 3% and 30.7 ± 0.5% respectively. MeOH extract, ethyl acetate fraction and butanol fraction exhibited antioxidant property with both two methods used. CONCLUSION: The results suggested that the extracts and compounds from roots of Pterocarpus erinaceus possessed local anti-inflammatory effect, antioxidant properties and inhibitor effect against lipoxygenase and lipid peroxidation activities. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016-11-23 /pmc/articles/PMC5411871/ /pubmed/28480397 http://dx.doi.org/10.21010/ajtcam.v14i1.21 Text en Copyright: © 2017 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License |
spellingShingle | Article Noufou, Ouédraogo Anne-Emmanuelle, Hay Claude W, Ouédraogo Jean Richard, Sawadogo W André, Tibiri Marius, Lompo Jean-baptiste, Nikiema Jean, Koudou Marie-Genevieve, Dijoux-Franca Pierre, Guissou Innocent BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATIONS OF EXTRACTS FROM PTEROCARPUS ERINACEUS POIR (FABACEAE) ROOT BARKS |
title | BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATIONS OF EXTRACTS FROM PTEROCARPUS ERINACEUS POIR (FABACEAE) ROOT BARKS |
title_full | BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATIONS OF EXTRACTS FROM PTEROCARPUS ERINACEUS POIR (FABACEAE) ROOT BARKS |
title_fullStr | BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATIONS OF EXTRACTS FROM PTEROCARPUS ERINACEUS POIR (FABACEAE) ROOT BARKS |
title_full_unstemmed | BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATIONS OF EXTRACTS FROM PTEROCARPUS ERINACEUS POIR (FABACEAE) ROOT BARKS |
title_short | BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATIONS OF EXTRACTS FROM PTEROCARPUS ERINACEUS POIR (FABACEAE) ROOT BARKS |
title_sort | biological and phytochemical investigations of extracts from pterocarpus erinaceus poir (fabaceae) root barks |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5411871/ https://www.ncbi.nlm.nih.gov/pubmed/28480397 http://dx.doi.org/10.21010/ajtcam.v14i1.21 |
work_keys_str_mv | AT noufououedraogo biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT anneemmanuellehay biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT claudewouedraogojean biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT richardsawadogow biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT andretibiri biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT mariuslompo biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT jeanbaptistenikiema biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT jeankoudou biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT mariegenevievedijouxfranca biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks AT pierreguissouinnocent biologicalandphytochemicalinvestigationsofextractsfrompterocarpuserinaceuspoirfabaceaerootbarks |