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Gene Electrotransfer: A Mechanistic Perspective
Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transien...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Science Publishers
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412002/ https://www.ncbi.nlm.nih.gov/pubmed/27029943 http://dx.doi.org/10.2174/1566523216666160331130040 |
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author | Rosazza, Christelle Meglic, Sasa Haberl Zumbusch, Andreas Rols, Marie-Pierre Miklavcic, Damijan |
author_facet | Rosazza, Christelle Meglic, Sasa Haberl Zumbusch, Andreas Rols, Marie-Pierre Miklavcic, Damijan |
author_sort | Rosazza, Christelle |
collection | PubMed |
description | Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability. Although gene electrotransfer has been extensively studied in in vitro and in vivo environments, the mechanisms by which DNA enters and navigates through cells are not fully understood. Here we present a comprehensive review of the body of knowledge concerning gene electrotransfer that has been accumulated over the last three decades. For that purpose, after briefly reviewing the medical applications that gene electrotransfer can provide, we outline membrane electropermeabilization, a key process for the delivery of DNA and smaller molecules. Since gene electrotransfer is a multipart process, we proceed our review in describing step by step our current understanding, with particular emphasis on DNA internalization and intracellular trafficking. Finally, we turn our attention to in vivo testing and methodology for gene electrotransfer. |
format | Online Article Text |
id | pubmed-5412002 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Bentham Science Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-54120022017-05-12 Gene Electrotransfer: A Mechanistic Perspective Rosazza, Christelle Meglic, Sasa Haberl Zumbusch, Andreas Rols, Marie-Pierre Miklavcic, Damijan Curr Gene Ther Article Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability. Although gene electrotransfer has been extensively studied in in vitro and in vivo environments, the mechanisms by which DNA enters and navigates through cells are not fully understood. Here we present a comprehensive review of the body of knowledge concerning gene electrotransfer that has been accumulated over the last three decades. For that purpose, after briefly reviewing the medical applications that gene electrotransfer can provide, we outline membrane electropermeabilization, a key process for the delivery of DNA and smaller molecules. Since gene electrotransfer is a multipart process, we proceed our review in describing step by step our current understanding, with particular emphasis on DNA internalization and intracellular trafficking. Finally, we turn our attention to in vivo testing and methodology for gene electrotransfer. Bentham Science Publishers 2016-04 2016-04 /pmc/articles/PMC5412002/ /pubmed/27029943 http://dx.doi.org/10.2174/1566523216666160331130040 Text en © 2016 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited. |
spellingShingle | Article Rosazza, Christelle Meglic, Sasa Haberl Zumbusch, Andreas Rols, Marie-Pierre Miklavcic, Damijan Gene Electrotransfer: A Mechanistic Perspective |
title | Gene Electrotransfer: A Mechanistic Perspective |
title_full | Gene Electrotransfer: A Mechanistic Perspective |
title_fullStr | Gene Electrotransfer: A Mechanistic Perspective |
title_full_unstemmed | Gene Electrotransfer: A Mechanistic Perspective |
title_short | Gene Electrotransfer: A Mechanistic Perspective |
title_sort | gene electrotransfer: a mechanistic perspective |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412002/ https://www.ncbi.nlm.nih.gov/pubmed/27029943 http://dx.doi.org/10.2174/1566523216666160331130040 |
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