Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures

BACKGROUND: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. METHODS: We tested the diagnostic accuracy of the IFA on 545 b...

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Detalles Bibliográficos
Autores principales: Dulsuk, Adul, Paksanont, Suporn, Sangchankoom, Adisak, Ekchariyawat, Peeraya, Phunpang, Rungnapa, Jutrakul, Yaowaruk, Chantratita, Narisara, West, T. Eoin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412066/
https://www.ncbi.nlm.nih.gov/pubmed/28115683
http://dx.doi.org/10.1093/trstmh/trw079
Descripción
Sumario:BACKGROUND: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. METHODS: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. RESULTS: Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25–51), p<0.0001. CONCLUSIONS: The IFA accurately expedites the diagnosis of melioidosis.

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