Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures

BACKGROUND: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. METHODS: We tested the diagnostic accuracy of the IFA on 545 b...

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Autores principales: Dulsuk, Adul, Paksanont, Suporn, Sangchankoom, Adisak, Ekchariyawat, Peeraya, Phunpang, Rungnapa, Jutrakul, Yaowaruk, Chantratita, Narisara, West, T. Eoin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412066/
https://www.ncbi.nlm.nih.gov/pubmed/28115683
http://dx.doi.org/10.1093/trstmh/trw079
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author Dulsuk, Adul
Paksanont, Suporn
Sangchankoom, Adisak
Ekchariyawat, Peeraya
Phunpang, Rungnapa
Jutrakul, Yaowaruk
Chantratita, Narisara
West, T. Eoin
author_facet Dulsuk, Adul
Paksanont, Suporn
Sangchankoom, Adisak
Ekchariyawat, Peeraya
Phunpang, Rungnapa
Jutrakul, Yaowaruk
Chantratita, Narisara
West, T. Eoin
author_sort Dulsuk, Adul
collection PubMed
description BACKGROUND: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. METHODS: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. RESULTS: Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25–51), p<0.0001. CONCLUSIONS: The IFA accurately expedites the diagnosis of melioidosis.
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spelling pubmed-54120662017-05-05 Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures Dulsuk, Adul Paksanont, Suporn Sangchankoom, Adisak Ekchariyawat, Peeraya Phunpang, Rungnapa Jutrakul, Yaowaruk Chantratita, Narisara West, T. Eoin Trans R Soc Trop Med Hyg Short Communications BACKGROUND: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. METHODS: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. RESULTS: Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25–51), p<0.0001. CONCLUSIONS: The IFA accurately expedites the diagnosis of melioidosis. Oxford University Press 2016-11 2017-02-01 /pmc/articles/PMC5412066/ /pubmed/28115683 http://dx.doi.org/10.1093/trstmh/trw079 Text en © The Author 2017. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Dulsuk, Adul
Paksanont, Suporn
Sangchankoom, Adisak
Ekchariyawat, Peeraya
Phunpang, Rungnapa
Jutrakul, Yaowaruk
Chantratita, Narisara
West, T. Eoin
Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures
title Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures
title_full Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures
title_fullStr Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures
title_full_unstemmed Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures
title_short Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures
title_sort validation of a monoclonal antibody-based immunofluorescent assay to detect burkholderia pseudomallei in blood cultures
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412066/
https://www.ncbi.nlm.nih.gov/pubmed/28115683
http://dx.doi.org/10.1093/trstmh/trw079
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