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PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES

BACKGROUND: Centaurea aegyptiaca L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the aerial parts of C. aegyptiaca metha...

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Autores principales: Bakr, Riham Omar, Mohamed, Shaza Abd El Halim, Ayoub, Nahla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412184/
https://www.ncbi.nlm.nih.gov/pubmed/28480370
http://dx.doi.org/10.21010/ajtcam.v13i6.19
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author Bakr, Riham Omar
Mohamed, Shaza Abd El Halim
Ayoub, Nahla
author_facet Bakr, Riham Omar
Mohamed, Shaza Abd El Halim
Ayoub, Nahla
author_sort Bakr, Riham Omar
collection PubMed
description BACKGROUND: Centaurea aegyptiaca L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the aerial parts of C. aegyptiaca methanolic extract utilizing liquid chromatography (LC) combined with electrospray ionization (ESI) tandem mass spectrometry (MS/MS). MATERIAL AND METHODS: Phenolic profile was researched utilizing LC-HRESI-MS-MS. Assessment of cytotoxic activity against four human cancer cell lines (Hep-G2; hepatocellular carcinoma cells, MCF-7; breast adenocarcinoma cells, and HCT-116; colon carcinoma and HELA; cervical carcinoma cells) was performed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Antiviral activity was surveyed utilizing cytopathic effect inhibition assay. RESULTS: A total of sixty-one compounds were tentatively distinguished (twenty-one phenolic acids and their derivatives, thirty-one flavonols and nine flavones) in the negative and positive modes. Centaurea aegyptiaca demonstrated outstanding results against Hep-G2, MCF-7, HCT-116 and HELA cell lines with IC(50) of 12.1, 30.9, 11.7 and 19.5 μg/mL respectively compared and doxorubicin as a reference drug. Weak antiviral activity was seen against hepatitis A virus (HAV) and no impact against herpes simplex virus type 1 (HSV 1). CONCLUSION: This study provides a better understanding of the chemistry of C. aegyptiaca that announces itself as a promising cytotoxic agent.
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spelling pubmed-54121842017-09-29 PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES Bakr, Riham Omar Mohamed, Shaza Abd El Halim Ayoub, Nahla Afr J Tradit Complement Altern Med Article BACKGROUND: Centaurea aegyptiaca L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the aerial parts of C. aegyptiaca methanolic extract utilizing liquid chromatography (LC) combined with electrospray ionization (ESI) tandem mass spectrometry (MS/MS). MATERIAL AND METHODS: Phenolic profile was researched utilizing LC-HRESI-MS-MS. Assessment of cytotoxic activity against four human cancer cell lines (Hep-G2; hepatocellular carcinoma cells, MCF-7; breast adenocarcinoma cells, and HCT-116; colon carcinoma and HELA; cervical carcinoma cells) was performed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Antiviral activity was surveyed utilizing cytopathic effect inhibition assay. RESULTS: A total of sixty-one compounds were tentatively distinguished (twenty-one phenolic acids and their derivatives, thirty-one flavonols and nine flavones) in the negative and positive modes. Centaurea aegyptiaca demonstrated outstanding results against Hep-G2, MCF-7, HCT-116 and HELA cell lines with IC(50) of 12.1, 30.9, 11.7 and 19.5 μg/mL respectively compared and doxorubicin as a reference drug. Weak antiviral activity was seen against hepatitis A virus (HAV) and no impact against herpes simplex virus type 1 (HSV 1). CONCLUSION: This study provides a better understanding of the chemistry of C. aegyptiaca that announces itself as a promising cytotoxic agent. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016-09-29 /pmc/articles/PMC5412184/ /pubmed/28480370 http://dx.doi.org/10.21010/ajtcam.v13i6.19 Text en Copyright: © 2016 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
spellingShingle Article
Bakr, Riham Omar
Mohamed, Shaza Abd El Halim
Ayoub, Nahla
PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES
title PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES
title_full PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES
title_fullStr PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES
title_full_unstemmed PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES
title_short PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES
title_sort phenolic profile of centaurea aegyptiaca l. growing in egypt and its cytotoxic and antiviral activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412184/
https://www.ncbi.nlm.nih.gov/pubmed/28480370
http://dx.doi.org/10.21010/ajtcam.v13i6.19
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