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BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolina

BACKGROUND: Mungbean [Vigna radiata (L.) Wilczek] is an important pulse crop globally. This imperative crop is severely affected by charcoal rot disease caused by Macrophomina phaseolina (Tassi) Goid. In the present study, the leaves of Calotropis procera L. were tested for their antifungal potentia...

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Autores principales: Waheed, Nidra, Jabeen, Khajista, Iqbal, Sumera, Javaid, Arshad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412188/
https://www.ncbi.nlm.nih.gov/pubmed/28480374
http://dx.doi.org/10.21010/ajtcam.v13i6.23
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author Waheed, Nidra
Jabeen, Khajista
Iqbal, Sumera
Javaid, Arshad
author_facet Waheed, Nidra
Jabeen, Khajista
Iqbal, Sumera
Javaid, Arshad
author_sort Waheed, Nidra
collection PubMed
description BACKGROUND: Mungbean [Vigna radiata (L.) Wilczek] is an important pulse crop globally. This imperative crop is severely affected by charcoal rot disease caused by Macrophomina phaseolina (Tassi) Goid. In the present study, the leaves of Calotropis procera L. were tested for their antifungal potential against M. phaseolina. MATERIALS AND METHODS: Various concentrations i.e. 1%, 2.5%, 4%, 5.5% and 7% of methanolic extract of C. procera leaves were prepared and their in vitro bioactivity was examined against the test fungus. Methnolic leaf extract was partitioned using n-hexane, chloroform, ethyl acetate and n-butanol and antifungal activity of each fraction was evaluated. n-Hexane fraction was subjected to GC-MS analysis. RESULTS: The higher concentration of methanolic leaf extract (7%) caused maximum inhibition in the diameter of M. phaseolina i.e. 38%. The n-hexane fraction of methanolic leaf extract was found to be the most effective against M. phaseolina. Seven compounds belonging to classes of chlorocarbon, aromatic hydrocarbon, azocompounds, aromatic carboxylic acids and fatty acids were identified in GC-MS analysis of n-hexane fraction. CONCLUSION: Antifungal activity of the methanolic leaf extract of C. procera might be due to the presence of the identified compounds in n-hexane fraction of methanolic leaf extract.
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spelling pubmed-54121882017-09-29 BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolina Waheed, Nidra Jabeen, Khajista Iqbal, Sumera Javaid, Arshad Afr J Tradit Complement Altern Med Article BACKGROUND: Mungbean [Vigna radiata (L.) Wilczek] is an important pulse crop globally. This imperative crop is severely affected by charcoal rot disease caused by Macrophomina phaseolina (Tassi) Goid. In the present study, the leaves of Calotropis procera L. were tested for their antifungal potential against M. phaseolina. MATERIALS AND METHODS: Various concentrations i.e. 1%, 2.5%, 4%, 5.5% and 7% of methanolic extract of C. procera leaves were prepared and their in vitro bioactivity was examined against the test fungus. Methnolic leaf extract was partitioned using n-hexane, chloroform, ethyl acetate and n-butanol and antifungal activity of each fraction was evaluated. n-Hexane fraction was subjected to GC-MS analysis. RESULTS: The higher concentration of methanolic leaf extract (7%) caused maximum inhibition in the diameter of M. phaseolina i.e. 38%. The n-hexane fraction of methanolic leaf extract was found to be the most effective against M. phaseolina. Seven compounds belonging to classes of chlorocarbon, aromatic hydrocarbon, azocompounds, aromatic carboxylic acids and fatty acids were identified in GC-MS analysis of n-hexane fraction. CONCLUSION: Antifungal activity of the methanolic leaf extract of C. procera might be due to the presence of the identified compounds in n-hexane fraction of methanolic leaf extract. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016-09-29 /pmc/articles/PMC5412188/ /pubmed/28480374 http://dx.doi.org/10.21010/ajtcam.v13i6.23 Text en Copyright: © 2016 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
spellingShingle Article
Waheed, Nidra
Jabeen, Khajista
Iqbal, Sumera
Javaid, Arshad
BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolina
title BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolina
title_full BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolina
title_fullStr BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolina
title_full_unstemmed BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolina
title_short BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolina
title_sort biopesticidal activity of calotropis procera l. against macrophomina phaseolina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412188/
https://www.ncbi.nlm.nih.gov/pubmed/28480374
http://dx.doi.org/10.21010/ajtcam.v13i6.23
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