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Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides

Multiple nanos genes have been characterized in several fishes, but the functional implications of their various expression patterns remain unclear. In this study, we identified and characterized four nanos genes from a hermaphroditic fish orange-spotted grouper, Epinephelus coioides. Ecnanos1a and...

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Autores principales: Sun, Zhi-Hui, Wang, Yang, Lu, Wei-Jia, Li, Zhi, Liu, Xiao-Chun, Li, Shui-Sheng, Zhou, Li, Gui, Jian-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412271/
https://www.ncbi.nlm.nih.gov/pubmed/28333083
http://dx.doi.org/10.3390/ijms18040685
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author Sun, Zhi-Hui
Wang, Yang
Lu, Wei-Jia
Li, Zhi
Liu, Xiao-Chun
Li, Shui-Sheng
Zhou, Li
Gui, Jian-Fang
author_facet Sun, Zhi-Hui
Wang, Yang
Lu, Wei-Jia
Li, Zhi
Liu, Xiao-Chun
Li, Shui-Sheng
Zhou, Li
Gui, Jian-Fang
author_sort Sun, Zhi-Hui
collection PubMed
description Multiple nanos genes have been characterized in several fishes, but the functional implications of their various expression patterns remain unclear. In this study, we identified and characterized four nanos genes from a hermaphroditic fish orange-spotted grouper, Epinephelus coioides. Ecnanos1a and Ecnanos1b show divergent expression patterns, and the dynamic expression change of Ecnanos1a in pituitaries during sex change is associated with testis differentiation and spermatogenesis. Ecnanos2 and Ecnanos3 might be germline stem cells (GSCs) and primordial germ cells (PGCs)-specific markers, respectively. Significantly, Ecnanos3 3′-untranslated region (UTR) is necessary for PGC specific expression, where a non-canonical “GCACGTTT” sequence is required for miR-430-mediated repression of Ecnanos3 RNA. Furthermore, grouper Dead end (Dnd) can relieve miR-430 repression in PGCs by associating with a 23 bp U-rich region (URR) in Ecnanos3 3′-UTR. The current study revealed the functional association of multiple nanos genes with PGC formation and germ cell development in orange-spotted grouper, and opened up new possibilities for developing biotechnologies through utilizing the associations between Ecnanos3 and PGCs or between Ecnanos2 and GSCs in the hermaphroditic fish.
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spelling pubmed-54122712017-05-05 Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides Sun, Zhi-Hui Wang, Yang Lu, Wei-Jia Li, Zhi Liu, Xiao-Chun Li, Shui-Sheng Zhou, Li Gui, Jian-Fang Int J Mol Sci Article Multiple nanos genes have been characterized in several fishes, but the functional implications of their various expression patterns remain unclear. In this study, we identified and characterized four nanos genes from a hermaphroditic fish orange-spotted grouper, Epinephelus coioides. Ecnanos1a and Ecnanos1b show divergent expression patterns, and the dynamic expression change of Ecnanos1a in pituitaries during sex change is associated with testis differentiation and spermatogenesis. Ecnanos2 and Ecnanos3 might be germline stem cells (GSCs) and primordial germ cells (PGCs)-specific markers, respectively. Significantly, Ecnanos3 3′-untranslated region (UTR) is necessary for PGC specific expression, where a non-canonical “GCACGTTT” sequence is required for miR-430-mediated repression of Ecnanos3 RNA. Furthermore, grouper Dead end (Dnd) can relieve miR-430 repression in PGCs by associating with a 23 bp U-rich region (URR) in Ecnanos3 3′-UTR. The current study revealed the functional association of multiple nanos genes with PGC formation and germ cell development in orange-spotted grouper, and opened up new possibilities for developing biotechnologies through utilizing the associations between Ecnanos3 and PGCs or between Ecnanos2 and GSCs in the hermaphroditic fish. MDPI 2017-03-23 /pmc/articles/PMC5412271/ /pubmed/28333083 http://dx.doi.org/10.3390/ijms18040685 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Zhi-Hui
Wang, Yang
Lu, Wei-Jia
Li, Zhi
Liu, Xiao-Chun
Li, Shui-Sheng
Zhou, Li
Gui, Jian-Fang
Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides
title Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides
title_full Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides
title_fullStr Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides
title_full_unstemmed Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides
title_short Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides
title_sort divergent expression patterns and function implications of four nanos genes in a hermaphroditic fish, epinephelus coioides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412271/
https://www.ncbi.nlm.nih.gov/pubmed/28333083
http://dx.doi.org/10.3390/ijms18040685
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