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Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation

Several Saccharomyces cerevisiae strains (flor yeasts) form a biofilm (flor velum) on the surface of Sherry wines after fermentation, when glucose is depleted. This flor velum is fundamental to biological aging of these particular wines. In this study, we identify abundant proteins in the formation...

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Autores principales: Moreno-García, Jaime, Mauricio, Juan Carlos, Moreno, Juan, García-Martínez, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412306/
https://www.ncbi.nlm.nih.gov/pubmed/28350350
http://dx.doi.org/10.3390/ijms18040720
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author Moreno-García, Jaime
Mauricio, Juan Carlos
Moreno, Juan
García-Martínez, Teresa
author_facet Moreno-García, Jaime
Mauricio, Juan Carlos
Moreno, Juan
García-Martínez, Teresa
author_sort Moreno-García, Jaime
collection PubMed
description Several Saccharomyces cerevisiae strains (flor yeasts) form a biofilm (flor velum) on the surface of Sherry wines after fermentation, when glucose is depleted. This flor velum is fundamental to biological aging of these particular wines. In this study, we identify abundant proteins in the formation of the biofilm of an industrial flor yeast strain. A database search to enrich flor yeast “biological process” and “cellular component” according to Gene Ontology Terminology (GO Terms) and, “pathways” was carried out. The most abundant proteins detected were largely involved in respiration, translation, stress damage prevention and repair, amino acid metabolism (glycine, isoleucine, leucine and arginine), glycolysis/gluconeogenesis and biosynthesis of vitamin B9 (folate). These proteins were located in cellular components as in the peroxisome, mitochondria, vacuole, cell wall and extracellular region; being these two last directly related with the flor formation. Proteins like Bgl2p, Gcv3p, Hyp2p, Mdh1p, Suc2p and Ygp1p were quantified in very high levels. This study reveals some expected processes and provides new and important information for the design of conditions and genetic constructions of flor yeasts for improving the cellular survival and, thus, to optimize biological aging of Sherry wine production.
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spelling pubmed-54123062017-05-05 Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation Moreno-García, Jaime Mauricio, Juan Carlos Moreno, Juan García-Martínez, Teresa Int J Mol Sci Article Several Saccharomyces cerevisiae strains (flor yeasts) form a biofilm (flor velum) on the surface of Sherry wines after fermentation, when glucose is depleted. This flor velum is fundamental to biological aging of these particular wines. In this study, we identify abundant proteins in the formation of the biofilm of an industrial flor yeast strain. A database search to enrich flor yeast “biological process” and “cellular component” according to Gene Ontology Terminology (GO Terms) and, “pathways” was carried out. The most abundant proteins detected were largely involved in respiration, translation, stress damage prevention and repair, amino acid metabolism (glycine, isoleucine, leucine and arginine), glycolysis/gluconeogenesis and biosynthesis of vitamin B9 (folate). These proteins were located in cellular components as in the peroxisome, mitochondria, vacuole, cell wall and extracellular region; being these two last directly related with the flor formation. Proteins like Bgl2p, Gcv3p, Hyp2p, Mdh1p, Suc2p and Ygp1p were quantified in very high levels. This study reveals some expected processes and provides new and important information for the design of conditions and genetic constructions of flor yeasts for improving the cellular survival and, thus, to optimize biological aging of Sherry wine production. MDPI 2017-03-28 /pmc/articles/PMC5412306/ /pubmed/28350350 http://dx.doi.org/10.3390/ijms18040720 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moreno-García, Jaime
Mauricio, Juan Carlos
Moreno, Juan
García-Martínez, Teresa
Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation
title Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation
title_full Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation
title_fullStr Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation
title_full_unstemmed Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation
title_short Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation
title_sort differential proteome analysis of a flor yeast strain under biofilm formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412306/
https://www.ncbi.nlm.nih.gov/pubmed/28350350
http://dx.doi.org/10.3390/ijms18040720
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