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The Protective Effect of Apigenin on Myocardial Injury in Diabetic Rats mediating Activation of the PPAR-γ Pathway

We substantiated the role of peroxisome proliferator-activated receptor-γ (PPAR-γ) activation in the protective effect of apigenin against the myocardial infarction (MI) in diabetic rats. Diabetes was induced by intraperitoneal administration of a single dose of streptozotocin (55 mg/kg). The study...

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Autores principales: Mahajan, Umesh B., Chandrayan, Govind, Patil, Chandragouda R., Arya, Dharamvir Singh, Suchal, Kapil, Agrawal, Yogeeta O., Ojha, Shreesh, Goyal, Sameer N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412341/
https://www.ncbi.nlm.nih.gov/pubmed/28375162
http://dx.doi.org/10.3390/ijms18040756
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author Mahajan, Umesh B.
Chandrayan, Govind
Patil, Chandragouda R.
Arya, Dharamvir Singh
Suchal, Kapil
Agrawal, Yogeeta O.
Ojha, Shreesh
Goyal, Sameer N.
author_facet Mahajan, Umesh B.
Chandrayan, Govind
Patil, Chandragouda R.
Arya, Dharamvir Singh
Suchal, Kapil
Agrawal, Yogeeta O.
Ojha, Shreesh
Goyal, Sameer N.
author_sort Mahajan, Umesh B.
collection PubMed
description We substantiated the role of peroxisome proliferator-activated receptor-γ (PPAR-γ) activation in the protective effect of apigenin against the myocardial infarction (MI) in diabetic rats. Diabetes was induced by intraperitoneal administration of a single dose of streptozotocin (55 mg/kg). The study groups included diabetic rats receiving vehicle, apigenin (75 mg/kg/day, orally), GW9662 (1 mg/kg/day, intraperitoneally), and a combination of apigenin and GW9662 for 14 days. The MI was induced in all the study groups except the diabetic control group by subcutaneous injection of 100 mg/kg/day of isoproterenol on the two terminal days. The diabetes and isoproterenol-induced MI was evident as a reduction in the maximal positive and negative rate of developed left ventricular pressure and an increase in the left ventricular end-diastolic pressure. The activities of creatine kinase on myocardial bundle (CK-MB) and lactate dehydrogenase (LDH) were also reduced. Apigenin treatment prevented the hemodynamic perturbations, restored the left ventricular function and reinstated a balanced redox status. It protected rats against an MI by attenuating myonecrosis, edema, cell death, and oxidative stress. GW9662, a PPAR-γ antagonist reversed the myocardial protection conferred by apigenin. Further, an increase in the PPAR-γ expression in the myocardium of the rats receiving apigenin reinforces the role of PPAR-γ pathway activation in the cardioprotective effects of apigenin.
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spelling pubmed-54123412017-05-05 The Protective Effect of Apigenin on Myocardial Injury in Diabetic Rats mediating Activation of the PPAR-γ Pathway Mahajan, Umesh B. Chandrayan, Govind Patil, Chandragouda R. Arya, Dharamvir Singh Suchal, Kapil Agrawal, Yogeeta O. Ojha, Shreesh Goyal, Sameer N. Int J Mol Sci Article We substantiated the role of peroxisome proliferator-activated receptor-γ (PPAR-γ) activation in the protective effect of apigenin against the myocardial infarction (MI) in diabetic rats. Diabetes was induced by intraperitoneal administration of a single dose of streptozotocin (55 mg/kg). The study groups included diabetic rats receiving vehicle, apigenin (75 mg/kg/day, orally), GW9662 (1 mg/kg/day, intraperitoneally), and a combination of apigenin and GW9662 for 14 days. The MI was induced in all the study groups except the diabetic control group by subcutaneous injection of 100 mg/kg/day of isoproterenol on the two terminal days. The diabetes and isoproterenol-induced MI was evident as a reduction in the maximal positive and negative rate of developed left ventricular pressure and an increase in the left ventricular end-diastolic pressure. The activities of creatine kinase on myocardial bundle (CK-MB) and lactate dehydrogenase (LDH) were also reduced. Apigenin treatment prevented the hemodynamic perturbations, restored the left ventricular function and reinstated a balanced redox status. It protected rats against an MI by attenuating myonecrosis, edema, cell death, and oxidative stress. GW9662, a PPAR-γ antagonist reversed the myocardial protection conferred by apigenin. Further, an increase in the PPAR-γ expression in the myocardium of the rats receiving apigenin reinforces the role of PPAR-γ pathway activation in the cardioprotective effects of apigenin. MDPI 2017-04-04 /pmc/articles/PMC5412341/ /pubmed/28375162 http://dx.doi.org/10.3390/ijms18040756 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mahajan, Umesh B.
Chandrayan, Govind
Patil, Chandragouda R.
Arya, Dharamvir Singh
Suchal, Kapil
Agrawal, Yogeeta O.
Ojha, Shreesh
Goyal, Sameer N.
The Protective Effect of Apigenin on Myocardial Injury in Diabetic Rats mediating Activation of the PPAR-γ Pathway
title The Protective Effect of Apigenin on Myocardial Injury in Diabetic Rats mediating Activation of the PPAR-γ Pathway
title_full The Protective Effect of Apigenin on Myocardial Injury in Diabetic Rats mediating Activation of the PPAR-γ Pathway
title_fullStr The Protective Effect of Apigenin on Myocardial Injury in Diabetic Rats mediating Activation of the PPAR-γ Pathway
title_full_unstemmed The Protective Effect of Apigenin on Myocardial Injury in Diabetic Rats mediating Activation of the PPAR-γ Pathway
title_short The Protective Effect of Apigenin on Myocardial Injury in Diabetic Rats mediating Activation of the PPAR-γ Pathway
title_sort protective effect of apigenin on myocardial injury in diabetic rats mediating activation of the ppar-γ pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412341/
https://www.ncbi.nlm.nih.gov/pubmed/28375162
http://dx.doi.org/10.3390/ijms18040756
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