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Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress
The F-box family is one of the largest gene families in plants that regulate diverse life processes, including salt responses. However, the knowledge of the soybean F-box genes and their roles in salt tolerance remains limited. Here, we conducted a genome-wide survey of the soybean F-box family, and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412402/ https://www.ncbi.nlm.nih.gov/pubmed/28417911 http://dx.doi.org/10.3390/ijms18040818 |
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author | Jia, Qi Xiao, Zhi-Xia Wong, Fuk-Ling Sun, Song Liang, Kang-Jing Lam, Hon-Ming |
author_facet | Jia, Qi Xiao, Zhi-Xia Wong, Fuk-Ling Sun, Song Liang, Kang-Jing Lam, Hon-Ming |
author_sort | Jia, Qi |
collection | PubMed |
description | The F-box family is one of the largest gene families in plants that regulate diverse life processes, including salt responses. However, the knowledge of the soybean F-box genes and their roles in salt tolerance remains limited. Here, we conducted a genome-wide survey of the soybean F-box family, and their expression analysis in response to salinity via in silico analysis of online RNA-sequencing (RNA-seq) data and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) to predict their potential functions. A total of 725 potential F-box proteins encoded by 509 genes were identified and classified into 9 subfamilies. The gene structures, conserved domains and chromosomal distributions were characterized. There are 76 pairs of duplicate genes identified, including genome-wide segmental and tandem duplication events, which lead to the expansion of the number of F-box genes. The in silico expression analysis showed that these genes would be involved in diverse developmental functions and play an important role in salt response. Our qRT-PCR analysis confirmed 12 salt-responding F-box genes. Overall, our results provide useful information on soybean F-box genes, especially their potential roles in salt tolerance. |
format | Online Article Text |
id | pubmed-5412402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54124022017-05-05 Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress Jia, Qi Xiao, Zhi-Xia Wong, Fuk-Ling Sun, Song Liang, Kang-Jing Lam, Hon-Ming Int J Mol Sci Article The F-box family is one of the largest gene families in plants that regulate diverse life processes, including salt responses. However, the knowledge of the soybean F-box genes and their roles in salt tolerance remains limited. Here, we conducted a genome-wide survey of the soybean F-box family, and their expression analysis in response to salinity via in silico analysis of online RNA-sequencing (RNA-seq) data and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) to predict their potential functions. A total of 725 potential F-box proteins encoded by 509 genes were identified and classified into 9 subfamilies. The gene structures, conserved domains and chromosomal distributions were characterized. There are 76 pairs of duplicate genes identified, including genome-wide segmental and tandem duplication events, which lead to the expansion of the number of F-box genes. The in silico expression analysis showed that these genes would be involved in diverse developmental functions and play an important role in salt response. Our qRT-PCR analysis confirmed 12 salt-responding F-box genes. Overall, our results provide useful information on soybean F-box genes, especially their potential roles in salt tolerance. MDPI 2017-04-12 /pmc/articles/PMC5412402/ /pubmed/28417911 http://dx.doi.org/10.3390/ijms18040818 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jia, Qi Xiao, Zhi-Xia Wong, Fuk-Ling Sun, Song Liang, Kang-Jing Lam, Hon-Ming Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress |
title | Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress |
title_full | Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress |
title_fullStr | Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress |
title_full_unstemmed | Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress |
title_short | Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress |
title_sort | genome-wide analyses of the soybean f-box gene family in response to salt stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412402/ https://www.ncbi.nlm.nih.gov/pubmed/28417911 http://dx.doi.org/10.3390/ijms18040818 |
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