Cargando…

Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress

The F-box family is one of the largest gene families in plants that regulate diverse life processes, including salt responses. However, the knowledge of the soybean F-box genes and their roles in salt tolerance remains limited. Here, we conducted a genome-wide survey of the soybean F-box family, and...

Descripción completa

Detalles Bibliográficos
Autores principales: Jia, Qi, Xiao, Zhi-Xia, Wong, Fuk-Ling, Sun, Song, Liang, Kang-Jing, Lam, Hon-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412402/
https://www.ncbi.nlm.nih.gov/pubmed/28417911
http://dx.doi.org/10.3390/ijms18040818
_version_ 1783232993445281792
author Jia, Qi
Xiao, Zhi-Xia
Wong, Fuk-Ling
Sun, Song
Liang, Kang-Jing
Lam, Hon-Ming
author_facet Jia, Qi
Xiao, Zhi-Xia
Wong, Fuk-Ling
Sun, Song
Liang, Kang-Jing
Lam, Hon-Ming
author_sort Jia, Qi
collection PubMed
description The F-box family is one of the largest gene families in plants that regulate diverse life processes, including salt responses. However, the knowledge of the soybean F-box genes and their roles in salt tolerance remains limited. Here, we conducted a genome-wide survey of the soybean F-box family, and their expression analysis in response to salinity via in silico analysis of online RNA-sequencing (RNA-seq) data and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) to predict their potential functions. A total of 725 potential F-box proteins encoded by 509 genes were identified and classified into 9 subfamilies. The gene structures, conserved domains and chromosomal distributions were characterized. There are 76 pairs of duplicate genes identified, including genome-wide segmental and tandem duplication events, which lead to the expansion of the number of F-box genes. The in silico expression analysis showed that these genes would be involved in diverse developmental functions and play an important role in salt response. Our qRT-PCR analysis confirmed 12 salt-responding F-box genes. Overall, our results provide useful information on soybean F-box genes, especially their potential roles in salt tolerance.
format Online
Article
Text
id pubmed-5412402
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-54124022017-05-05 Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress Jia, Qi Xiao, Zhi-Xia Wong, Fuk-Ling Sun, Song Liang, Kang-Jing Lam, Hon-Ming Int J Mol Sci Article The F-box family is one of the largest gene families in plants that regulate diverse life processes, including salt responses. However, the knowledge of the soybean F-box genes and their roles in salt tolerance remains limited. Here, we conducted a genome-wide survey of the soybean F-box family, and their expression analysis in response to salinity via in silico analysis of online RNA-sequencing (RNA-seq) data and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) to predict their potential functions. A total of 725 potential F-box proteins encoded by 509 genes were identified and classified into 9 subfamilies. The gene structures, conserved domains and chromosomal distributions were characterized. There are 76 pairs of duplicate genes identified, including genome-wide segmental and tandem duplication events, which lead to the expansion of the number of F-box genes. The in silico expression analysis showed that these genes would be involved in diverse developmental functions and play an important role in salt response. Our qRT-PCR analysis confirmed 12 salt-responding F-box genes. Overall, our results provide useful information on soybean F-box genes, especially their potential roles in salt tolerance. MDPI 2017-04-12 /pmc/articles/PMC5412402/ /pubmed/28417911 http://dx.doi.org/10.3390/ijms18040818 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jia, Qi
Xiao, Zhi-Xia
Wong, Fuk-Ling
Sun, Song
Liang, Kang-Jing
Lam, Hon-Ming
Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress
title Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress
title_full Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress
title_fullStr Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress
title_full_unstemmed Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress
title_short Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress
title_sort genome-wide analyses of the soybean f-box gene family in response to salt stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412402/
https://www.ncbi.nlm.nih.gov/pubmed/28417911
http://dx.doi.org/10.3390/ijms18040818
work_keys_str_mv AT jiaqi genomewideanalysesofthesoybeanfboxgenefamilyinresponsetosaltstress
AT xiaozhixia genomewideanalysesofthesoybeanfboxgenefamilyinresponsetosaltstress
AT wongfukling genomewideanalysesofthesoybeanfboxgenefamilyinresponsetosaltstress
AT sunsong genomewideanalysesofthesoybeanfboxgenefamilyinresponsetosaltstress
AT liangkangjing genomewideanalysesofthesoybeanfboxgenefamilyinresponsetosaltstress
AT lamhonming genomewideanalysesofthesoybeanfboxgenefamilyinresponsetosaltstress