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Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release
The primary cilium is a highly conserved organelle housing specialized molecules responsible for receiving and processing extracellular signals. A recently discovered property shared across many cilia is the ability to release small vesicles called ectosomes, which are used for exchanging protein an...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412563/ https://www.ncbi.nlm.nih.gov/pubmed/28381413 http://dx.doi.org/10.1083/jcb.201608081 |
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author | Salinas, Raquel Y. Pearring, Jillian N. Ding, Jin-Dong Spencer, William J. Hao, Ying Arshavsky, Vadim Y. |
author_facet | Salinas, Raquel Y. Pearring, Jillian N. Ding, Jin-Dong Spencer, William J. Hao, Ying Arshavsky, Vadim Y. |
author_sort | Salinas, Raquel Y. |
collection | PubMed |
description | The primary cilium is a highly conserved organelle housing specialized molecules responsible for receiving and processing extracellular signals. A recently discovered property shared across many cilia is the ability to release small vesicles called ectosomes, which are used for exchanging protein and genetic material among cells. In this study, we report a novel role for ciliary ectosomes in building the elaborate photoreceptor outer segment filled with hundreds of tightly packed “disc” membranes. We demonstrate that the photoreceptor cilium has an innate ability to release massive amounts of ectosomes. However, this process is suppressed by the disc-specific protein peripherin, which enables retained ectosomes to be morphed into discs. This new function of peripherin is performed independently from its well-established role in maintaining the high curvature of disc edges, and each function is fulfilled by a separate part of peripherin’s molecule. Our findings explain how the outer segment structure evolved from the primary cilium to provide photoreceptor cells with vast membrane surfaces for efficient light capture. |
format | Online Article Text |
id | pubmed-5412563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54125632017-11-01 Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release Salinas, Raquel Y. Pearring, Jillian N. Ding, Jin-Dong Spencer, William J. Hao, Ying Arshavsky, Vadim Y. J Cell Biol Research Articles The primary cilium is a highly conserved organelle housing specialized molecules responsible for receiving and processing extracellular signals. A recently discovered property shared across many cilia is the ability to release small vesicles called ectosomes, which are used for exchanging protein and genetic material among cells. In this study, we report a novel role for ciliary ectosomes in building the elaborate photoreceptor outer segment filled with hundreds of tightly packed “disc” membranes. We demonstrate that the photoreceptor cilium has an innate ability to release massive amounts of ectosomes. However, this process is suppressed by the disc-specific protein peripherin, which enables retained ectosomes to be morphed into discs. This new function of peripherin is performed independently from its well-established role in maintaining the high curvature of disc edges, and each function is fulfilled by a separate part of peripherin’s molecule. Our findings explain how the outer segment structure evolved from the primary cilium to provide photoreceptor cells with vast membrane surfaces for efficient light capture. The Rockefeller University Press 2017-05-01 /pmc/articles/PMC5412563/ /pubmed/28381413 http://dx.doi.org/10.1083/jcb.201608081 Text en © 2017 Salinas et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Salinas, Raquel Y. Pearring, Jillian N. Ding, Jin-Dong Spencer, William J. Hao, Ying Arshavsky, Vadim Y. Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release |
title | Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release |
title_full | Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release |
title_fullStr | Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release |
title_full_unstemmed | Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release |
title_short | Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release |
title_sort | photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412563/ https://www.ncbi.nlm.nih.gov/pubmed/28381413 http://dx.doi.org/10.1083/jcb.201608081 |
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