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Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications

OBJECTIVE: Poly [2-methacryloyloxyethyl phosphoryl choline (MPC)-co-n-buthyl methacrylate (BMA)-co-p-nitrophenyl-oxycrabonyl poly ethylene glycol-methacrylate (ME- ONP)] (PMBN), a biocompatible terpolymer, is a unique polymer with applications that range from drug delivery systems (DDS) to scaffolds...

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Autores principales: Motamed Fath, Puria, Yazdian, Fatemeh, Jamjah, Rogayyeh, Ebrahimi Hosseinzadeh, Bahman, Rahimnezhad, Maede, Sahraeian, Razi, Hatamian, Ashrafalsadat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412785/
https://www.ncbi.nlm.nih.gov/pubmed/28670519
http://dx.doi.org/10.22074/cellj.2016.4119
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author Motamed Fath, Puria
Yazdian, Fatemeh
Jamjah, Rogayyeh
Ebrahimi Hosseinzadeh, Bahman
Rahimnezhad, Maede
Sahraeian, Razi
Hatamian, Ashrafalsadat
author_facet Motamed Fath, Puria
Yazdian, Fatemeh
Jamjah, Rogayyeh
Ebrahimi Hosseinzadeh, Bahman
Rahimnezhad, Maede
Sahraeian, Razi
Hatamian, Ashrafalsadat
author_sort Motamed Fath, Puria
collection PubMed
description OBJECTIVE: Poly [2-methacryloyloxyethyl phosphoryl choline (MPC)-co-n-buthyl methacrylate (BMA)-co-p-nitrophenyl-oxycrabonyl poly ethylene glycol-methacrylate (ME- ONP)] (PMBN), a biocompatible terpolymer, is a unique polymer with applications that range from drug delivery systems (DDS) to scaffolds and biomedical devices. In this research, we have prepared a monomer of p-nitrophenyl-oxycarbonyl poly (ethylene glycol) methacrylate (MEONP) to synthesize this polymer. Next, we designed and prepared a smart, water soluble, amphiphilic PMBN polymer composed of MPC, BMA, and MEONP. MATERIALS AND METHODS: In this experimental study, we dissolved MPC (4 mmol, 40% mole fraction), BMA (5 mmol, 50% mole fraction), and MEONP (1 mmol, 10% mole fraction) in 20 ml of dry ethanol in two necked flasks equipped with inlet-outlet gas. The structural characteristics of the synthesized monomer and polymer were determined by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (H-NMR), dynamic light scattering (DLS), gel permeation chromatography (GPC), scanning electron microscope (SEM), and transmission electron microscope (TEM) analyses for the first time. We treated the polymer with two different cell lines to determine its biocompatibility. RESULTS: FT-IR and H-NMR analyses confirmed the synthesis of the polymer. The size of polymer was approximately 40 nm with a molecular weight (MW) of 52 kDa, which would be excellent for a nano carrier. Microscopic analyses showed that the polymer was rodshaped. This polymer had no toxicity for individual cells. CONCLUSION: We report here, for the first time, the full properties of the PMBN polymer. The approximately 40 nm size with an acceptable zeta potential range of -8.47, PDI of 0.1, and rod-shaped structure indicated adequate parameters of a nanopolymer for nano bioapplications. We used this polymer to design a new smart nano carrier to treat leukemia stem cells based on a target DDS as a type of bio-application.
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spelling pubmed-54127852017-07-01 Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications Motamed Fath, Puria Yazdian, Fatemeh Jamjah, Rogayyeh Ebrahimi Hosseinzadeh, Bahman Rahimnezhad, Maede Sahraeian, Razi Hatamian, Ashrafalsadat Cell J Original Article OBJECTIVE: Poly [2-methacryloyloxyethyl phosphoryl choline (MPC)-co-n-buthyl methacrylate (BMA)-co-p-nitrophenyl-oxycrabonyl poly ethylene glycol-methacrylate (ME- ONP)] (PMBN), a biocompatible terpolymer, is a unique polymer with applications that range from drug delivery systems (DDS) to scaffolds and biomedical devices. In this research, we have prepared a monomer of p-nitrophenyl-oxycarbonyl poly (ethylene glycol) methacrylate (MEONP) to synthesize this polymer. Next, we designed and prepared a smart, water soluble, amphiphilic PMBN polymer composed of MPC, BMA, and MEONP. MATERIALS AND METHODS: In this experimental study, we dissolved MPC (4 mmol, 40% mole fraction), BMA (5 mmol, 50% mole fraction), and MEONP (1 mmol, 10% mole fraction) in 20 ml of dry ethanol in two necked flasks equipped with inlet-outlet gas. The structural characteristics of the synthesized monomer and polymer were determined by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (H-NMR), dynamic light scattering (DLS), gel permeation chromatography (GPC), scanning electron microscope (SEM), and transmission electron microscope (TEM) analyses for the first time. We treated the polymer with two different cell lines to determine its biocompatibility. RESULTS: FT-IR and H-NMR analyses confirmed the synthesis of the polymer. The size of polymer was approximately 40 nm with a molecular weight (MW) of 52 kDa, which would be excellent for a nano carrier. Microscopic analyses showed that the polymer was rodshaped. This polymer had no toxicity for individual cells. CONCLUSION: We report here, for the first time, the full properties of the PMBN polymer. The approximately 40 nm size with an acceptable zeta potential range of -8.47, PDI of 0.1, and rod-shaped structure indicated adequate parameters of a nanopolymer for nano bioapplications. We used this polymer to design a new smart nano carrier to treat leukemia stem cells based on a target DDS as a type of bio-application. Royan Institute 2017 2017-02-22 /pmc/articles/PMC5412785/ /pubmed/28670519 http://dx.doi.org/10.22074/cellj.2016.4119 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Motamed Fath, Puria
Yazdian, Fatemeh
Jamjah, Rogayyeh
Ebrahimi Hosseinzadeh, Bahman
Rahimnezhad, Maede
Sahraeian, Razi
Hatamian, Ashrafalsadat
Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications
title Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications
title_full Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications
title_fullStr Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications
title_full_unstemmed Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications
title_short Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications
title_sort synthesis and characterization of pmbn as a biocompatible nanopolymer for bio-applications
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412785/
https://www.ncbi.nlm.nih.gov/pubmed/28670519
http://dx.doi.org/10.22074/cellj.2016.4119
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