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Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease
Both the incidence and prevalence of inflammatory bowel disease (IBD) is increasing globally; in the industrialized world up to 0.5% of the population are affected and around 4.2 million individuals suffer from IBD in Europe and North America combined. Successful engraftment in experimental colitis...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412884/ https://www.ncbi.nlm.nih.gov/pubmed/28283650 http://dx.doi.org/10.15252/emmm.201607260 |
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author | Holmberg, Fredrik EO Seidelin, Jakob B Yin, Xiaolei Mead, Benjamin E Tong, Zhixiang Li, Yuan Karp, Jeffrey M Nielsen, Ole H |
author_facet | Holmberg, Fredrik EO Seidelin, Jakob B Yin, Xiaolei Mead, Benjamin E Tong, Zhixiang Li, Yuan Karp, Jeffrey M Nielsen, Ole H |
author_sort | Holmberg, Fredrik EO |
collection | PubMed |
description | Both the incidence and prevalence of inflammatory bowel disease (IBD) is increasing globally; in the industrialized world up to 0.5% of the population are affected and around 4.2 million individuals suffer from IBD in Europe and North America combined. Successful engraftment in experimental colitis models suggests that intestinal stem cell transplantation could constitute a novel treatment strategy to re‐establish mucosal barrier function in patients with severe disease. Intestinal stem cells can be grown in vitro in organoid structures, though only a fraction of the cells contained are stem cells with regenerative capabilities. Hence, techniques to enrich stem cell populations are being pursued through the development of multiple two‐dimensional and three‐dimensional culture protocols, as well as co‐culture techniques and multiple growth medium compositions. Moreover, research in support matrices allowing for efficient clinical application is in progress. In vitro culture is accomplished by modulating the signaling pathways fundamental for the stem cell niche with a suitable culture matrix to provide additional contact‐dependent stimuli and structural support. The aim of this review was to discuss medium compositions and support matrices for optimal intestinal stem cell culture, as well as potential modifications to advance clinical use in IBD. |
format | Online Article Text |
id | pubmed-5412884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54128842017-05-03 Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease Holmberg, Fredrik EO Seidelin, Jakob B Yin, Xiaolei Mead, Benjamin E Tong, Zhixiang Li, Yuan Karp, Jeffrey M Nielsen, Ole H EMBO Mol Med Reviews Both the incidence and prevalence of inflammatory bowel disease (IBD) is increasing globally; in the industrialized world up to 0.5% of the population are affected and around 4.2 million individuals suffer from IBD in Europe and North America combined. Successful engraftment in experimental colitis models suggests that intestinal stem cell transplantation could constitute a novel treatment strategy to re‐establish mucosal barrier function in patients with severe disease. Intestinal stem cells can be grown in vitro in organoid structures, though only a fraction of the cells contained are stem cells with regenerative capabilities. Hence, techniques to enrich stem cell populations are being pursued through the development of multiple two‐dimensional and three‐dimensional culture protocols, as well as co‐culture techniques and multiple growth medium compositions. Moreover, research in support matrices allowing for efficient clinical application is in progress. In vitro culture is accomplished by modulating the signaling pathways fundamental for the stem cell niche with a suitable culture matrix to provide additional contact‐dependent stimuli and structural support. The aim of this review was to discuss medium compositions and support matrices for optimal intestinal stem cell culture, as well as potential modifications to advance clinical use in IBD. John Wiley and Sons Inc. 2017-03-10 2017-05 /pmc/articles/PMC5412884/ /pubmed/28283650 http://dx.doi.org/10.15252/emmm.201607260 Text en © 2017 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the Creative Commons Attribution 4.0 (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Reviews Holmberg, Fredrik EO Seidelin, Jakob B Yin, Xiaolei Mead, Benjamin E Tong, Zhixiang Li, Yuan Karp, Jeffrey M Nielsen, Ole H Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease |
title | Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease |
title_full | Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease |
title_fullStr | Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease |
title_full_unstemmed | Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease |
title_short | Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease |
title_sort | culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease |
topic | Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412884/ https://www.ncbi.nlm.nih.gov/pubmed/28283650 http://dx.doi.org/10.15252/emmm.201607260 |
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