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PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines

Psoriasis is a chronic and persistent inflammatory skin disease seriously affecting the quality of human life. In this study, we reported an ancient formula of Chinese folk medicine, the natural plant antimicrobial solution (PAMs) for its anti-inflammatory effects and proposed the primary mechanisms...

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Autores principales: Dou, Rongkun, Liu, Zongying, Yuan, Xue, Xiangfei, Danzhou, Bai, Ruixue, Bi, Zhenfei, Yang, Piao, Yang, Yalan, Dong, Yinsong, Su, Wei, Li, Diqiang, Mao, Canquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413058/
https://www.ncbi.nlm.nih.gov/pubmed/28464025
http://dx.doi.org/10.1371/journal.pone.0176823
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author Dou, Rongkun
Liu, Zongying
Yuan, Xue
Xiangfei, Danzhou
Bai, Ruixue
Bi, Zhenfei
Yang, Piao
Yang, Yalan
Dong, Yinsong
Su, Wei
Li, Diqiang
Mao, Canquan
author_facet Dou, Rongkun
Liu, Zongying
Yuan, Xue
Xiangfei, Danzhou
Bai, Ruixue
Bi, Zhenfei
Yang, Piao
Yang, Yalan
Dong, Yinsong
Su, Wei
Li, Diqiang
Mao, Canquan
author_sort Dou, Rongkun
collection PubMed
description Psoriasis is a chronic and persistent inflammatory skin disease seriously affecting the quality of human life. In this study, we reported an ancient formula of Chinese folk medicine, the natural plant antimicrobial solution (PAMs) for its anti-inflammatory effects and proposed the primary mechanisms on inhibiting the inflammatory response in TNF-α/IFN-γ-induced HaCaT cells and imiquimod-induced psoriasis-like skin disease mouse model. Two main functional components of hydroxysafflor Yellow A and allantoin in PAMs were quantified by HPLC to be 94.2±2.2 and 262.9±12.5 μg/mL respectively. PAMs could significantly reduce the gene expression and inflammatory cytokines production of Macrophage-Derived Chemokine (MDC), IL-8 and IL-6 in TNF-α/IFN-γ-induced HaCaT cells. PAMs also significantly ameliorates the psoriatic-like symptoms in a mouse model with the evaluation scores for both the single (scales, thickness, erythema) and cumulative features were in the order of blank control < Dexamethasone < PAMs < 50% ethanol < model groups. The results were further confirmed by hematoxylin-eosin staining, RT-qPCR and immunohistochemistry. The down-regulated gene expression of IL-8, TNF-α, ICAM-1 and IL-23 in mouse tissues was consistent with the results from those of the HaCaT cells. The inhibition of psoriasis-like skin inflammation by PAMs was correlated with the inactivation of the translocation of P65 protein into cellular nucleus, indicating the inhibition of the inflammatory NF-κB signaling pathway. Taken together, these findings suggest that PAMs may be a promising drug candidate for the treatment of inflammatory skin disorders, such as psoriasis.
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spelling pubmed-54130582017-05-14 PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines Dou, Rongkun Liu, Zongying Yuan, Xue Xiangfei, Danzhou Bai, Ruixue Bi, Zhenfei Yang, Piao Yang, Yalan Dong, Yinsong Su, Wei Li, Diqiang Mao, Canquan PLoS One Research Article Psoriasis is a chronic and persistent inflammatory skin disease seriously affecting the quality of human life. In this study, we reported an ancient formula of Chinese folk medicine, the natural plant antimicrobial solution (PAMs) for its anti-inflammatory effects and proposed the primary mechanisms on inhibiting the inflammatory response in TNF-α/IFN-γ-induced HaCaT cells and imiquimod-induced psoriasis-like skin disease mouse model. Two main functional components of hydroxysafflor Yellow A and allantoin in PAMs were quantified by HPLC to be 94.2±2.2 and 262.9±12.5 μg/mL respectively. PAMs could significantly reduce the gene expression and inflammatory cytokines production of Macrophage-Derived Chemokine (MDC), IL-8 and IL-6 in TNF-α/IFN-γ-induced HaCaT cells. PAMs also significantly ameliorates the psoriatic-like symptoms in a mouse model with the evaluation scores for both the single (scales, thickness, erythema) and cumulative features were in the order of blank control < Dexamethasone < PAMs < 50% ethanol < model groups. The results were further confirmed by hematoxylin-eosin staining, RT-qPCR and immunohistochemistry. The down-regulated gene expression of IL-8, TNF-α, ICAM-1 and IL-23 in mouse tissues was consistent with the results from those of the HaCaT cells. The inhibition of psoriasis-like skin inflammation by PAMs was correlated with the inactivation of the translocation of P65 protein into cellular nucleus, indicating the inhibition of the inflammatory NF-κB signaling pathway. Taken together, these findings suggest that PAMs may be a promising drug candidate for the treatment of inflammatory skin disorders, such as psoriasis. Public Library of Science 2017-05-02 /pmc/articles/PMC5413058/ /pubmed/28464025 http://dx.doi.org/10.1371/journal.pone.0176823 Text en © 2017 Dou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dou, Rongkun
Liu, Zongying
Yuan, Xue
Xiangfei, Danzhou
Bai, Ruixue
Bi, Zhenfei
Yang, Piao
Yang, Yalan
Dong, Yinsong
Su, Wei
Li, Diqiang
Mao, Canquan
PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines
title PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines
title_full PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines
title_fullStr PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines
title_full_unstemmed PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines
title_short PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines
title_sort pams ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of nf-κb and production of inflammatory cytokines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413058/
https://www.ncbi.nlm.nih.gov/pubmed/28464025
http://dx.doi.org/10.1371/journal.pone.0176823
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