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micro-RNA screening and prediction model construction for diagnosis of salt-sensitive essential hypertension
Commonly used tests for diagnosis of salt-sensitive hypertension (SSH) are complex and time-consuming, so new methods are required. Many studies have demonstrated roles for miRNAs in hypertension; however, the diagnostic value of miRNAs has yet to be determined for human SSH. In this study, we exami...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413218/ https://www.ncbi.nlm.nih.gov/pubmed/28445253 http://dx.doi.org/10.1097/MD.0000000000006417 |
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author | Qi, Han Liu, Zheng Liu, Bin Cao, Han Sun, Weiping Yan, Yuxiang Zhang, Ling |
author_facet | Qi, Han Liu, Zheng Liu, Bin Cao, Han Sun, Weiping Yan, Yuxiang Zhang, Ling |
author_sort | Qi, Han |
collection | PubMed |
description | Commonly used tests for diagnosis of salt-sensitive hypertension (SSH) are complex and time-consuming, so new methods are required. Many studies have demonstrated roles for miRNAs in hypertension; however, the diagnostic value of miRNAs has yet to be determined for human SSH. In this study, we examined miRNA expression profiles by initial high-throughput miRNA sequencing of samples from patients with salt-sensitive and salt-resistant hypertension (SSH and SRH, respectively; n = 6, both groups), followed by validation by quantitative real-time polymerase chain reaction (qRT-PCR) in a larger cohort (n = 91). We also evaluated differences in baseline characteristics (e.g., age, sex, body mass index, consumption of specific foods) between the SSH and SRH groups. Of 36 miRNAs identified as differentially expressed between SSH and SRH groups by RNA-Seq, 8 were analyzed by qRT-PCR. There were significant differences in the expression levels of hsa-miR-361-5p and hsa-miR-362-5p between the 2 groups (P = .023 and.049, respectively). In addition, there were significant differences in sauce and poultry consumption between the 2 groups (P = .004 and.001, respectively). The areas under the curve (AUC) determined by receptor operating characteristic (ROC) analysis for hsa-miR-361–5p and all 8 miRNAs were 0.793 (95% CI, 0.698–0.888; sensitivity = 73.9%, specificity = 74.4%; P < .001) and 0.836 (95% CI, 0.749–0.922; sensitivity = 80.4%, specificity = 81.4%; P < .001), respectively, when sauce and poultry consumption were included in the models. Assay feasibility and economic considerations make hsa-miR-361-5p combined with the dietary factors the preferred markers for diagnosis of SSH. |
format | Online Article Text |
id | pubmed-5413218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-54132182017-05-05 micro-RNA screening and prediction model construction for diagnosis of salt-sensitive essential hypertension Qi, Han Liu, Zheng Liu, Bin Cao, Han Sun, Weiping Yan, Yuxiang Zhang, Ling Medicine (Baltimore) 4400 Commonly used tests for diagnosis of salt-sensitive hypertension (SSH) are complex and time-consuming, so new methods are required. Many studies have demonstrated roles for miRNAs in hypertension; however, the diagnostic value of miRNAs has yet to be determined for human SSH. In this study, we examined miRNA expression profiles by initial high-throughput miRNA sequencing of samples from patients with salt-sensitive and salt-resistant hypertension (SSH and SRH, respectively; n = 6, both groups), followed by validation by quantitative real-time polymerase chain reaction (qRT-PCR) in a larger cohort (n = 91). We also evaluated differences in baseline characteristics (e.g., age, sex, body mass index, consumption of specific foods) between the SSH and SRH groups. Of 36 miRNAs identified as differentially expressed between SSH and SRH groups by RNA-Seq, 8 were analyzed by qRT-PCR. There were significant differences in the expression levels of hsa-miR-361-5p and hsa-miR-362-5p between the 2 groups (P = .023 and.049, respectively). In addition, there were significant differences in sauce and poultry consumption between the 2 groups (P = .004 and.001, respectively). The areas under the curve (AUC) determined by receptor operating characteristic (ROC) analysis for hsa-miR-361–5p and all 8 miRNAs were 0.793 (95% CI, 0.698–0.888; sensitivity = 73.9%, specificity = 74.4%; P < .001) and 0.836 (95% CI, 0.749–0.922; sensitivity = 80.4%, specificity = 81.4%; P < .001), respectively, when sauce and poultry consumption were included in the models. Assay feasibility and economic considerations make hsa-miR-361-5p combined with the dietary factors the preferred markers for diagnosis of SSH. Wolters Kluwer Health 2017-04-28 /pmc/articles/PMC5413218/ /pubmed/28445253 http://dx.doi.org/10.1097/MD.0000000000006417 Text en Copyright © 2017 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 |
spellingShingle | 4400 Qi, Han Liu, Zheng Liu, Bin Cao, Han Sun, Weiping Yan, Yuxiang Zhang, Ling micro-RNA screening and prediction model construction for diagnosis of salt-sensitive essential hypertension |
title | micro-RNA screening and prediction model construction for diagnosis of salt-sensitive essential hypertension |
title_full | micro-RNA screening and prediction model construction for diagnosis of salt-sensitive essential hypertension |
title_fullStr | micro-RNA screening and prediction model construction for diagnosis of salt-sensitive essential hypertension |
title_full_unstemmed | micro-RNA screening and prediction model construction for diagnosis of salt-sensitive essential hypertension |
title_short | micro-RNA screening and prediction model construction for diagnosis of salt-sensitive essential hypertension |
title_sort | micro-rna screening and prediction model construction for diagnosis of salt-sensitive essential hypertension |
topic | 4400 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413218/ https://www.ncbi.nlm.nih.gov/pubmed/28445253 http://dx.doi.org/10.1097/MD.0000000000006417 |
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