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The role of STAT3 and AhR in the differentiation of CD4(+) T cells into Th17 and Treg cells
BACKGROUND: This study aimed to investigate the role of aryl hydrocarbon receptor (AhR) and STAT3 gene during the differentiation of cluster of differentiation (CD)4(+) T cells into T helper (Th)17 and T regulatory (Treg) cells. METHODS: First, CD4(+) T cells were isolated from the spleen of BALB/c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413224/ https://www.ncbi.nlm.nih.gov/pubmed/28445259 http://dx.doi.org/10.1097/MD.0000000000006615 |
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author | Liu, Xingxing Hu, Hui Fan, Heng Zuo, Dongmei Shou, Zhexing Liao, Yi Nan, Zhen Tang, Qing |
author_facet | Liu, Xingxing Hu, Hui Fan, Heng Zuo, Dongmei Shou, Zhexing Liao, Yi Nan, Zhen Tang, Qing |
author_sort | Liu, Xingxing |
collection | PubMed |
description | BACKGROUND: This study aimed to investigate the role of aryl hydrocarbon receptor (AhR) and STAT3 gene during the differentiation of cluster of differentiation (CD)4(+) T cells into T helper (Th)17 and T regulatory (Treg) cells. METHODS: First, CD4(+) T cells were isolated from the spleen of BALB/c mice. Then, stable CD4(+) T cells expressing STAT3 shRNA were constructed. CD4(+) T cells were assigned to one of the following treatments: Th17 group: antibodies against CD3 and CD28, 2.5 ng/mL transforming growth factor β (TGF-β)1, 30 ng/mL interleukin (IL)-6, and 30 ng/mL IL-23; 6-formylindolo[3,2-b]carbazole (FICZ) group: antibodies against CD3 and CD28, 2.5 ng/mL TGF-β1, 30 ng/mL IL-6, 30 ng/mL IL-23, and 100 nM FICZ; FICZ + STAT3 RNAi group (shSTAT3 group): antibodies against CD3 and CD28, 2.5 ng/mL TGF-β1, 30 ng/mL IL-6, 30 ng/mL IL-23, 100 nM FICZ, and STAT3 RNAi; naphthoflavone group: antibodies against CD3 and CD28, 2.5 ng/mL TGF-β1, 30 ng/mL IL-6, 30 ng/mL IL-23, and 3 μM naphthoflavone; 5) no antibodies were added in the control group. Later, the proportions of Th17 and Treg cells in each group were measured by flow cytometry; phospho-STAT3 and -STAT5 levels were measured by western blotting; and AhR, STAT3, STAT5, receptor-related orphan nuclear receptor γt (RORγt), FOXP3, T-cell receptor (TCR), CD25, IL-6R, IL-10, and IL-17 mRNA levels were also measured by real-time PCR. RESULT: Th17 cells showed a rise and Treg cells showed a decrease in the FICZ group, but revised in the shSTAT3 group and the naphthoflavone group. Significant differences were observed in CD25, IL-6R, IL-10, and IL-17 mRNA levels among different groups. CONCLUSION: STAT3 may cooperate with AhR to regulate the differentiation of both Th17 and Treg cells. |
format | Online Article Text |
id | pubmed-5413224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-54132242017-05-05 The role of STAT3 and AhR in the differentiation of CD4(+) T cells into Th17 and Treg cells Liu, Xingxing Hu, Hui Fan, Heng Zuo, Dongmei Shou, Zhexing Liao, Yi Nan, Zhen Tang, Qing Medicine (Baltimore) 3600 BACKGROUND: This study aimed to investigate the role of aryl hydrocarbon receptor (AhR) and STAT3 gene during the differentiation of cluster of differentiation (CD)4(+) T cells into T helper (Th)17 and T regulatory (Treg) cells. METHODS: First, CD4(+) T cells were isolated from the spleen of BALB/c mice. Then, stable CD4(+) T cells expressing STAT3 shRNA were constructed. CD4(+) T cells were assigned to one of the following treatments: Th17 group: antibodies against CD3 and CD28, 2.5 ng/mL transforming growth factor β (TGF-β)1, 30 ng/mL interleukin (IL)-6, and 30 ng/mL IL-23; 6-formylindolo[3,2-b]carbazole (FICZ) group: antibodies against CD3 and CD28, 2.5 ng/mL TGF-β1, 30 ng/mL IL-6, 30 ng/mL IL-23, and 100 nM FICZ; FICZ + STAT3 RNAi group (shSTAT3 group): antibodies against CD3 and CD28, 2.5 ng/mL TGF-β1, 30 ng/mL IL-6, 30 ng/mL IL-23, 100 nM FICZ, and STAT3 RNAi; naphthoflavone group: antibodies against CD3 and CD28, 2.5 ng/mL TGF-β1, 30 ng/mL IL-6, 30 ng/mL IL-23, and 3 μM naphthoflavone; 5) no antibodies were added in the control group. Later, the proportions of Th17 and Treg cells in each group were measured by flow cytometry; phospho-STAT3 and -STAT5 levels were measured by western blotting; and AhR, STAT3, STAT5, receptor-related orphan nuclear receptor γt (RORγt), FOXP3, T-cell receptor (TCR), CD25, IL-6R, IL-10, and IL-17 mRNA levels were also measured by real-time PCR. RESULT: Th17 cells showed a rise and Treg cells showed a decrease in the FICZ group, but revised in the shSTAT3 group and the naphthoflavone group. Significant differences were observed in CD25, IL-6R, IL-10, and IL-17 mRNA levels among different groups. CONCLUSION: STAT3 may cooperate with AhR to regulate the differentiation of both Th17 and Treg cells. Wolters Kluwer Health 2017-04-28 /pmc/articles/PMC5413224/ /pubmed/28445259 http://dx.doi.org/10.1097/MD.0000000000006615 Text en Copyright © 2017 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 |
spellingShingle | 3600 Liu, Xingxing Hu, Hui Fan, Heng Zuo, Dongmei Shou, Zhexing Liao, Yi Nan, Zhen Tang, Qing The role of STAT3 and AhR in the differentiation of CD4(+) T cells into Th17 and Treg cells |
title | The role of STAT3 and AhR in the differentiation of CD4(+) T cells into Th17 and Treg cells |
title_full | The role of STAT3 and AhR in the differentiation of CD4(+) T cells into Th17 and Treg cells |
title_fullStr | The role of STAT3 and AhR in the differentiation of CD4(+) T cells into Th17 and Treg cells |
title_full_unstemmed | The role of STAT3 and AhR in the differentiation of CD4(+) T cells into Th17 and Treg cells |
title_short | The role of STAT3 and AhR in the differentiation of CD4(+) T cells into Th17 and Treg cells |
title_sort | role of stat3 and ahr in the differentiation of cd4(+) t cells into th17 and treg cells |
topic | 3600 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413224/ https://www.ncbi.nlm.nih.gov/pubmed/28445259 http://dx.doi.org/10.1097/MD.0000000000006615 |
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