Cargando…

VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran

Foods with animal origins play a substantial role in the transmission of Helicobacter pylori. The present investigation was carried out to study the vacA and cagA genotypes status of H. pylori isolated from various types of meat samples. Two hundred and twenty meat samples were collected and culture...

Descripción completa

Detalles Bibliográficos
Autores principales: Gilani, Ali, Razavilar, Vadood, Rokni, Nordahr, Rahimi, Ebrahim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413315/
https://www.ncbi.nlm.nih.gov/pubmed/28473901
_version_ 1783233165770358784
author Gilani, Ali
Razavilar, Vadood
Rokni, Nordahr
Rahimi, Ebrahim
author_facet Gilani, Ali
Razavilar, Vadood
Rokni, Nordahr
Rahimi, Ebrahim
author_sort Gilani, Ali
collection PubMed
description Foods with animal origins play a substantial role in the transmission of Helicobacter pylori. The present investigation was carried out to study the vacA and cagA genotypes status of H. pylori isolated from various types of meat samples. Two hundred and twenty meat samples were collected and cultured. H. pylori-positive strains were analyzed for the presence of vacA and cagA genotypes. Eleven out of 220 (5.00%) samples were positive for H. pylori. Findings were confirmed by nested PCR. Prevalence of H. pylori in the meat samples of slaughterhouses and butcheries were 72.20% and 27.70%, respectively. The most commonly detected genotypes in the meat samples of slaughterhouses and butcheries were vacA m1a (66.66%) and vacA s1a (37.50%), respectively. The S1am1a was the most commonly detected genotype. Meat sampled from butcheries had the higher prevalence of H. pylori and its genotypes than those of slaughterhouses (p < 0.05). Results showed that meat samples could be the potential sources of virulent strains of H. pylori. Application of sanitary measures in the storage, transportation and sale of meat is essential for reducing the levels of H. pylori cross contamination.
format Online
Article
Text
id pubmed-5413315
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Urmia University Press
record_format MEDLINE/PubMed
spelling pubmed-54133152017-05-04 VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran Gilani, Ali Razavilar, Vadood Rokni, Nordahr Rahimi, Ebrahim Vet Res Forum Original Article Foods with animal origins play a substantial role in the transmission of Helicobacter pylori. The present investigation was carried out to study the vacA and cagA genotypes status of H. pylori isolated from various types of meat samples. Two hundred and twenty meat samples were collected and cultured. H. pylori-positive strains were analyzed for the presence of vacA and cagA genotypes. Eleven out of 220 (5.00%) samples were positive for H. pylori. Findings were confirmed by nested PCR. Prevalence of H. pylori in the meat samples of slaughterhouses and butcheries were 72.20% and 27.70%, respectively. The most commonly detected genotypes in the meat samples of slaughterhouses and butcheries were vacA m1a (66.66%) and vacA s1a (37.50%), respectively. The S1am1a was the most commonly detected genotype. Meat sampled from butcheries had the higher prevalence of H. pylori and its genotypes than those of slaughterhouses (p < 0.05). Results showed that meat samples could be the potential sources of virulent strains of H. pylori. Application of sanitary measures in the storage, transportation and sale of meat is essential for reducing the levels of H. pylori cross contamination. Urmia University Press 2017 2017-03-15 /pmc/articles/PMC5413315/ /pubmed/28473901 Text en © 2017 Urmia University. All rights reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Gilani, Ali
Razavilar, Vadood
Rokni, Nordahr
Rahimi, Ebrahim
VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran
title VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran
title_full VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran
title_fullStr VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran
title_full_unstemmed VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran
title_short VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran
title_sort vaca and caga genotypes of helicobacter pylori isolated from raw meat in isfahan province, iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413315/
https://www.ncbi.nlm.nih.gov/pubmed/28473901
work_keys_str_mv AT gilaniali vacaandcagagenotypesofhelicobacterpyloriisolatedfromrawmeatinisfahanprovinceiran
AT razavilarvadood vacaandcagagenotypesofhelicobacterpyloriisolatedfromrawmeatinisfahanprovinceiran
AT rokninordahr vacaandcagagenotypesofhelicobacterpyloriisolatedfromrawmeatinisfahanprovinceiran
AT rahimiebrahim vacaandcagagenotypesofhelicobacterpyloriisolatedfromrawmeatinisfahanprovinceiran