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TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
The spores of pathogenic bacteria are involved in host entry and the initial encounter with the host immune system. How bacterial spores interact with host immunity, however, remains poorly understood. Here, we show that the spores of Bacillus anthracis (BA), the etiologic agent of anthrax, possess...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413331/ https://www.ncbi.nlm.nih.gov/pubmed/28400473 http://dx.doi.org/10.1084/jem.20161141 |
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author | Choo, Min-Kyung Sano, Yasuyo Kim, Changhoon Yasuda, Kei Li, Xiao-Dong Lin, Xin Stenzel-Poore, Mary Alexopoulou, Lena Ghosh, Sankar Latz, Eicke Rifkin, Ian R. Chen, Zhijian J. Stewart, George C. Chong, Hyonyong Park, Jin Mo |
author_facet | Choo, Min-Kyung Sano, Yasuyo Kim, Changhoon Yasuda, Kei Li, Xiao-Dong Lin, Xin Stenzel-Poore, Mary Alexopoulou, Lena Ghosh, Sankar Latz, Eicke Rifkin, Ian R. Chen, Zhijian J. Stewart, George C. Chong, Hyonyong Park, Jin Mo |
author_sort | Choo, Min-Kyung |
collection | PubMed |
description | The spores of pathogenic bacteria are involved in host entry and the initial encounter with the host immune system. How bacterial spores interact with host immunity, however, remains poorly understood. Here, we show that the spores of Bacillus anthracis (BA), the etiologic agent of anthrax, possess an intrinsic ability to induce host immune responses. This immunostimulatory activity is attributable to high amounts of RNA present in the spore surface layer. RNA-sensing TLRs, TLR7, and TLR13 in mice and their human counterparts, are responsible for detecting and triggering the host cell response to BA spores, whereas TLR2 mediates the sensing of vegetative BA. BA spores, but not vegetative BA, induce type I IFN (IFN-I) production. Although TLR signaling in itself affords protection against BA, spore RNA–induced IFN-I signaling is disruptive to BA clearance. Our study suggests a role for bacterial spore–associated RNA in microbial pathogenesis and illustrates a little known aspect of interactions between the host and spore-forming bacteria. |
format | Online Article Text |
id | pubmed-5413331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54133312017-11-01 TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects Choo, Min-Kyung Sano, Yasuyo Kim, Changhoon Yasuda, Kei Li, Xiao-Dong Lin, Xin Stenzel-Poore, Mary Alexopoulou, Lena Ghosh, Sankar Latz, Eicke Rifkin, Ian R. Chen, Zhijian J. Stewart, George C. Chong, Hyonyong Park, Jin Mo J Exp Med Research Articles The spores of pathogenic bacteria are involved in host entry and the initial encounter with the host immune system. How bacterial spores interact with host immunity, however, remains poorly understood. Here, we show that the spores of Bacillus anthracis (BA), the etiologic agent of anthrax, possess an intrinsic ability to induce host immune responses. This immunostimulatory activity is attributable to high amounts of RNA present in the spore surface layer. RNA-sensing TLRs, TLR7, and TLR13 in mice and their human counterparts, are responsible for detecting and triggering the host cell response to BA spores, whereas TLR2 mediates the sensing of vegetative BA. BA spores, but not vegetative BA, induce type I IFN (IFN-I) production. Although TLR signaling in itself affords protection against BA, spore RNA–induced IFN-I signaling is disruptive to BA clearance. Our study suggests a role for bacterial spore–associated RNA in microbial pathogenesis and illustrates a little known aspect of interactions between the host and spore-forming bacteria. The Rockefeller University Press 2017-05-01 /pmc/articles/PMC5413331/ /pubmed/28400473 http://dx.doi.org/10.1084/jem.20161141 Text en © 2017 Choo et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Choo, Min-Kyung Sano, Yasuyo Kim, Changhoon Yasuda, Kei Li, Xiao-Dong Lin, Xin Stenzel-Poore, Mary Alexopoulou, Lena Ghosh, Sankar Latz, Eicke Rifkin, Ian R. Chen, Zhijian J. Stewart, George C. Chong, Hyonyong Park, Jin Mo TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects |
title | TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects |
title_full | TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects |
title_fullStr | TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects |
title_full_unstemmed | TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects |
title_short | TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects |
title_sort | tlr sensing of bacterial spore-associated rna triggers host immune responses with detrimental effects |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413331/ https://www.ncbi.nlm.nih.gov/pubmed/28400473 http://dx.doi.org/10.1084/jem.20161141 |
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