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TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects

The spores of pathogenic bacteria are involved in host entry and the initial encounter with the host immune system. How bacterial spores interact with host immunity, however, remains poorly understood. Here, we show that the spores of Bacillus anthracis (BA), the etiologic agent of anthrax, possess...

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Autores principales: Choo, Min-Kyung, Sano, Yasuyo, Kim, Changhoon, Yasuda, Kei, Li, Xiao-Dong, Lin, Xin, Stenzel-Poore, Mary, Alexopoulou, Lena, Ghosh, Sankar, Latz, Eicke, Rifkin, Ian R., Chen, Zhijian J., Stewart, George C., Chong, Hyonyong, Park, Jin Mo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413331/
https://www.ncbi.nlm.nih.gov/pubmed/28400473
http://dx.doi.org/10.1084/jem.20161141
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author Choo, Min-Kyung
Sano, Yasuyo
Kim, Changhoon
Yasuda, Kei
Li, Xiao-Dong
Lin, Xin
Stenzel-Poore, Mary
Alexopoulou, Lena
Ghosh, Sankar
Latz, Eicke
Rifkin, Ian R.
Chen, Zhijian J.
Stewart, George C.
Chong, Hyonyong
Park, Jin Mo
author_facet Choo, Min-Kyung
Sano, Yasuyo
Kim, Changhoon
Yasuda, Kei
Li, Xiao-Dong
Lin, Xin
Stenzel-Poore, Mary
Alexopoulou, Lena
Ghosh, Sankar
Latz, Eicke
Rifkin, Ian R.
Chen, Zhijian J.
Stewart, George C.
Chong, Hyonyong
Park, Jin Mo
author_sort Choo, Min-Kyung
collection PubMed
description The spores of pathogenic bacteria are involved in host entry and the initial encounter with the host immune system. How bacterial spores interact with host immunity, however, remains poorly understood. Here, we show that the spores of Bacillus anthracis (BA), the etiologic agent of anthrax, possess an intrinsic ability to induce host immune responses. This immunostimulatory activity is attributable to high amounts of RNA present in the spore surface layer. RNA-sensing TLRs, TLR7, and TLR13 in mice and their human counterparts, are responsible for detecting and triggering the host cell response to BA spores, whereas TLR2 mediates the sensing of vegetative BA. BA spores, but not vegetative BA, induce type I IFN (IFN-I) production. Although TLR signaling in itself affords protection against BA, spore RNA–induced IFN-I signaling is disruptive to BA clearance. Our study suggests a role for bacterial spore–associated RNA in microbial pathogenesis and illustrates a little known aspect of interactions between the host and spore-forming bacteria.
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spelling pubmed-54133312017-11-01 TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects Choo, Min-Kyung Sano, Yasuyo Kim, Changhoon Yasuda, Kei Li, Xiao-Dong Lin, Xin Stenzel-Poore, Mary Alexopoulou, Lena Ghosh, Sankar Latz, Eicke Rifkin, Ian R. Chen, Zhijian J. Stewart, George C. Chong, Hyonyong Park, Jin Mo J Exp Med Research Articles The spores of pathogenic bacteria are involved in host entry and the initial encounter with the host immune system. How bacterial spores interact with host immunity, however, remains poorly understood. Here, we show that the spores of Bacillus anthracis (BA), the etiologic agent of anthrax, possess an intrinsic ability to induce host immune responses. This immunostimulatory activity is attributable to high amounts of RNA present in the spore surface layer. RNA-sensing TLRs, TLR7, and TLR13 in mice and their human counterparts, are responsible for detecting and triggering the host cell response to BA spores, whereas TLR2 mediates the sensing of vegetative BA. BA spores, but not vegetative BA, induce type I IFN (IFN-I) production. Although TLR signaling in itself affords protection against BA, spore RNA–induced IFN-I signaling is disruptive to BA clearance. Our study suggests a role for bacterial spore–associated RNA in microbial pathogenesis and illustrates a little known aspect of interactions between the host and spore-forming bacteria. The Rockefeller University Press 2017-05-01 /pmc/articles/PMC5413331/ /pubmed/28400473 http://dx.doi.org/10.1084/jem.20161141 Text en © 2017 Choo et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Choo, Min-Kyung
Sano, Yasuyo
Kim, Changhoon
Yasuda, Kei
Li, Xiao-Dong
Lin, Xin
Stenzel-Poore, Mary
Alexopoulou, Lena
Ghosh, Sankar
Latz, Eicke
Rifkin, Ian R.
Chen, Zhijian J.
Stewart, George C.
Chong, Hyonyong
Park, Jin Mo
TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
title TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
title_full TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
title_fullStr TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
title_full_unstemmed TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
title_short TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
title_sort tlr sensing of bacterial spore-associated rna triggers host immune responses with detrimental effects
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413331/
https://www.ncbi.nlm.nih.gov/pubmed/28400473
http://dx.doi.org/10.1084/jem.20161141
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