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Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses

This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of ∼40 nm in diameter were injec...

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Autores principales: Daurer, Benedikt J., Okamoto, Kenta, Bielecki, Johan, Maia, Filipe R. N. C., Mühlig, Kerstin, Seibert, M. Marvin, Hantke, Max F., Nettelblad, Carl, Benner, W. Henry, Svenda, Martin, Tîmneanu, Nicuşor, Ekeberg, Tomas, Loh, N. Duane, Pietrini, Alberto, Zani, Alessandro, Rath, Asawari D., Westphal, Daniel, Kirian, Richard A., Awel, Salah, Wiedorn, Max O., van der Schot, Gijs, Carlsson, Gunilla H., Hasse, Dirk, Sellberg, Jonas A., Barty, Anton, Andreasson, Jakob, Boutet, Sébastien, Williams, Garth, Koglin, Jason, Andersson, Inger, Hajdu, Janos, Larsson, Daniel S. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5414399/
https://www.ncbi.nlm.nih.gov/pubmed/28512572
http://dx.doi.org/10.1107/S2052252517003591
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author Daurer, Benedikt J.
Okamoto, Kenta
Bielecki, Johan
Maia, Filipe R. N. C.
Mühlig, Kerstin
Seibert, M. Marvin
Hantke, Max F.
Nettelblad, Carl
Benner, W. Henry
Svenda, Martin
Tîmneanu, Nicuşor
Ekeberg, Tomas
Loh, N. Duane
Pietrini, Alberto
Zani, Alessandro
Rath, Asawari D.
Westphal, Daniel
Kirian, Richard A.
Awel, Salah
Wiedorn, Max O.
van der Schot, Gijs
Carlsson, Gunilla H.
Hasse, Dirk
Sellberg, Jonas A.
Barty, Anton
Andreasson, Jakob
Boutet, Sébastien
Williams, Garth
Koglin, Jason
Andersson, Inger
Hajdu, Janos
Larsson, Daniel S. D.
author_facet Daurer, Benedikt J.
Okamoto, Kenta
Bielecki, Johan
Maia, Filipe R. N. C.
Mühlig, Kerstin
Seibert, M. Marvin
Hantke, Max F.
Nettelblad, Carl
Benner, W. Henry
Svenda, Martin
Tîmneanu, Nicuşor
Ekeberg, Tomas
Loh, N. Duane
Pietrini, Alberto
Zani, Alessandro
Rath, Asawari D.
Westphal, Daniel
Kirian, Richard A.
Awel, Salah
Wiedorn, Max O.
van der Schot, Gijs
Carlsson, Gunilla H.
Hasse, Dirk
Sellberg, Jonas A.
Barty, Anton
Andreasson, Jakob
Boutet, Sébastien
Williams, Garth
Koglin, Jason
Andersson, Inger
Hajdu, Janos
Larsson, Daniel S. D.
author_sort Daurer, Benedikt J.
collection PubMed
description This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of ∼40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ∼35 to ∼300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 10(12) photons per µm(2) per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.
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spelling pubmed-54143992017-05-16 Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses Daurer, Benedikt J. Okamoto, Kenta Bielecki, Johan Maia, Filipe R. N. C. Mühlig, Kerstin Seibert, M. Marvin Hantke, Max F. Nettelblad, Carl Benner, W. Henry Svenda, Martin Tîmneanu, Nicuşor Ekeberg, Tomas Loh, N. Duane Pietrini, Alberto Zani, Alessandro Rath, Asawari D. Westphal, Daniel Kirian, Richard A. Awel, Salah Wiedorn, Max O. van der Schot, Gijs Carlsson, Gunilla H. Hasse, Dirk Sellberg, Jonas A. Barty, Anton Andreasson, Jakob Boutet, Sébastien Williams, Garth Koglin, Jason Andersson, Inger Hajdu, Janos Larsson, Daniel S. D. IUCrJ Research Papers This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of ∼40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from ∼35 to ∼300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 × 10(12) photons per µm(2) per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers. International Union of Crystallography 2017-04-07 /pmc/articles/PMC5414399/ /pubmed/28512572 http://dx.doi.org/10.1107/S2052252517003591 Text en © Benedikt J. Daurer et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Papers
Daurer, Benedikt J.
Okamoto, Kenta
Bielecki, Johan
Maia, Filipe R. N. C.
Mühlig, Kerstin
Seibert, M. Marvin
Hantke, Max F.
Nettelblad, Carl
Benner, W. Henry
Svenda, Martin
Tîmneanu, Nicuşor
Ekeberg, Tomas
Loh, N. Duane
Pietrini, Alberto
Zani, Alessandro
Rath, Asawari D.
Westphal, Daniel
Kirian, Richard A.
Awel, Salah
Wiedorn, Max O.
van der Schot, Gijs
Carlsson, Gunilla H.
Hasse, Dirk
Sellberg, Jonas A.
Barty, Anton
Andreasson, Jakob
Boutet, Sébastien
Williams, Garth
Koglin, Jason
Andersson, Inger
Hajdu, Janos
Larsson, Daniel S. D.
Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
title Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
title_full Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
title_fullStr Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
title_full_unstemmed Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
title_short Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
title_sort experimental strategies for imaging bioparticles with femtosecond hard x-ray pulses
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5414399/
https://www.ncbi.nlm.nih.gov/pubmed/28512572
http://dx.doi.org/10.1107/S2052252517003591
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