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Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice

Background/Aims. The effects of cholecalciferol supplementation on the course of diabetes in humans and animals need to be better understood. Therefore, this study investigated the effect of short-term cholecalciferol supplementation on biochemical and hematological parameters in mice. Methods. Male...

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Autores principales: Bella, Leonardo M., Fieri, Isis, Tessaro, Fernando H. G., Nolasco, Eduardo L., Nunes, Fernanda P. B., Ferreira, Sabrina S., Azevedo, Carolina B., Martins, Joilson O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5414504/
https://www.ncbi.nlm.nih.gov/pubmed/28503574
http://dx.doi.org/10.1155/2017/7651815
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author Bella, Leonardo M.
Fieri, Isis
Tessaro, Fernando H. G.
Nolasco, Eduardo L.
Nunes, Fernanda P. B.
Ferreira, Sabrina S.
Azevedo, Carolina B.
Martins, Joilson O.
author_facet Bella, Leonardo M.
Fieri, Isis
Tessaro, Fernando H. G.
Nolasco, Eduardo L.
Nunes, Fernanda P. B.
Ferreira, Sabrina S.
Azevedo, Carolina B.
Martins, Joilson O.
author_sort Bella, Leonardo M.
collection PubMed
description Background/Aims. The effects of cholecalciferol supplementation on the course of diabetes in humans and animals need to be better understood. Therefore, this study investigated the effect of short-term cholecalciferol supplementation on biochemical and hematological parameters in mice. Methods. Male diabetic (alloxan, 60 mg/kg i.v., 10 days) and nondiabetic mice were supplemented with cholecalciferol for seven days. The following parameters were determined: serum levels of 25-hydroxyvitamin D, phosphorus, calcium, urea, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, red blood cell count, white blood cell count (WBC), hematocrit, hemoglobin, differential cell counts of peritoneal lavage (PeL), and bronchoalveolar lavage (BAL) fluids and morphological analysis of lung, kidney, and liver tissues. Results. Relative to controls, cholecalciferol supplementation increased serum levels of 25-hydroxyvitamin D, calcium, hemoglobin, hematocrit, and red blood cell counts and decreased leukocyte cell counts of PeL and BAL fluids in diabetic mice. Diabetic mice that were not treated with cholecalciferol had lower serum calcium and albumin levels and hemoglobin, WBC, and mononuclear blood cell counts and higher serum creatinine and urea levels than controls. Conclusion. Our results suggest that cholecalciferol supplementation improves the hematological parameters and reduces leukocyte migration into the PeL and BAL lavage of diabetic mice.
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spelling pubmed-54145042017-05-14 Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice Bella, Leonardo M. Fieri, Isis Tessaro, Fernando H. G. Nolasco, Eduardo L. Nunes, Fernanda P. B. Ferreira, Sabrina S. Azevedo, Carolina B. Martins, Joilson O. Biomed Res Int Research Article Background/Aims. The effects of cholecalciferol supplementation on the course of diabetes in humans and animals need to be better understood. Therefore, this study investigated the effect of short-term cholecalciferol supplementation on biochemical and hematological parameters in mice. Methods. Male diabetic (alloxan, 60 mg/kg i.v., 10 days) and nondiabetic mice were supplemented with cholecalciferol for seven days. The following parameters were determined: serum levels of 25-hydroxyvitamin D, phosphorus, calcium, urea, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, red blood cell count, white blood cell count (WBC), hematocrit, hemoglobin, differential cell counts of peritoneal lavage (PeL), and bronchoalveolar lavage (BAL) fluids and morphological analysis of lung, kidney, and liver tissues. Results. Relative to controls, cholecalciferol supplementation increased serum levels of 25-hydroxyvitamin D, calcium, hemoglobin, hematocrit, and red blood cell counts and decreased leukocyte cell counts of PeL and BAL fluids in diabetic mice. Diabetic mice that were not treated with cholecalciferol had lower serum calcium and albumin levels and hemoglobin, WBC, and mononuclear blood cell counts and higher serum creatinine and urea levels than controls. Conclusion. Our results suggest that cholecalciferol supplementation improves the hematological parameters and reduces leukocyte migration into the PeL and BAL lavage of diabetic mice. Hindawi 2017 2017-04-19 /pmc/articles/PMC5414504/ /pubmed/28503574 http://dx.doi.org/10.1155/2017/7651815 Text en Copyright © 2017 Leonardo M. Bella et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bella, Leonardo M.
Fieri, Isis
Tessaro, Fernando H. G.
Nolasco, Eduardo L.
Nunes, Fernanda P. B.
Ferreira, Sabrina S.
Azevedo, Carolina B.
Martins, Joilson O.
Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice
title Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice
title_full Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice
title_fullStr Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice
title_full_unstemmed Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice
title_short Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice
title_sort vitamin d modulates hematological parameters and cell migration into peritoneal and pulmonary cavities in alloxan-diabetic mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5414504/
https://www.ncbi.nlm.nih.gov/pubmed/28503574
http://dx.doi.org/10.1155/2017/7651815
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