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Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community

Routine manufacture, detonation and disposal of explosives in land and groundwater have resulted in complete pollution. Explosives are xenobiotic compounds, being toxic to biological systems, and their recalcitrance leads to persistence in the environment. The methods currently used for the remediat...

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Detalles Bibliográficos
Autores principales: Al-Dhabaan, Fahad Abdullah M., Bakhali, Ali Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415123/
https://www.ncbi.nlm.nih.gov/pubmed/28490963
http://dx.doi.org/10.1016/j.sjbs.2016.01.043
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author Al-Dhabaan, Fahad Abdullah M.
Bakhali, Ali Hassan
author_facet Al-Dhabaan, Fahad Abdullah M.
Bakhali, Ali Hassan
author_sort Al-Dhabaan, Fahad Abdullah M.
collection PubMed
description Routine manufacture, detonation and disposal of explosives in land and groundwater have resulted in complete pollution. Explosives are xenobiotic compounds, being toxic to biological systems, and their recalcitrance leads to persistence in the environment. The methods currently used for the remediation of explosive contaminated sites are expensive and can result in the formation of toxic products. The present study aimed to investigate the bacterial strains using the Biolog plates in the soil from the Riyadh community. The microbial strains were isolated using the spread plate technique and were identified using the Biolog method. In this study we have analyzed from bacterial families of soil samples, obtained from the different sites in 5 regions at Explosive Institute. Our results conclude that Biolog MicroPlates were developed for the rapid identification of bacterial isolates by sole-carbon source utilization and can be used for the identification of bacteria. Out of five communities, only four families of bacteria indicate that the microbial community lacks significant diversity in region one from the Riyadh community in Saudi Arabia. More studies are needed to be carried out in different regions to validate our results.
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spelling pubmed-54151232017-05-10 Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community Al-Dhabaan, Fahad Abdullah M. Bakhali, Ali Hassan Saudi J Biol Sci Original Article Routine manufacture, detonation and disposal of explosives in land and groundwater have resulted in complete pollution. Explosives are xenobiotic compounds, being toxic to biological systems, and their recalcitrance leads to persistence in the environment. The methods currently used for the remediation of explosive contaminated sites are expensive and can result in the formation of toxic products. The present study aimed to investigate the bacterial strains using the Biolog plates in the soil from the Riyadh community. The microbial strains were isolated using the spread plate technique and were identified using the Biolog method. In this study we have analyzed from bacterial families of soil samples, obtained from the different sites in 5 regions at Explosive Institute. Our results conclude that Biolog MicroPlates were developed for the rapid identification of bacterial isolates by sole-carbon source utilization and can be used for the identification of bacteria. Out of five communities, only four families of bacteria indicate that the microbial community lacks significant diversity in region one from the Riyadh community in Saudi Arabia. More studies are needed to be carried out in different regions to validate our results. Elsevier 2017-05 2016-02-01 /pmc/articles/PMC5415123/ /pubmed/28490963 http://dx.doi.org/10.1016/j.sjbs.2016.01.043 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Al-Dhabaan, Fahad Abdullah M.
Bakhali, Ali Hassan
Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community
title Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community
title_full Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community
title_fullStr Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community
title_full_unstemmed Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community
title_short Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community
title_sort analysis of the bacterial strains using biolog plates in the contaminated soil from riyadh community
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415123/
https://www.ncbi.nlm.nih.gov/pubmed/28490963
http://dx.doi.org/10.1016/j.sjbs.2016.01.043
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