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Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community
Routine manufacture, detonation and disposal of explosives in land and groundwater have resulted in complete pollution. Explosives are xenobiotic compounds, being toxic to biological systems, and their recalcitrance leads to persistence in the environment. The methods currently used for the remediat...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415123/ https://www.ncbi.nlm.nih.gov/pubmed/28490963 http://dx.doi.org/10.1016/j.sjbs.2016.01.043 |
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author | Al-Dhabaan, Fahad Abdullah M. Bakhali, Ali Hassan |
author_facet | Al-Dhabaan, Fahad Abdullah M. Bakhali, Ali Hassan |
author_sort | Al-Dhabaan, Fahad Abdullah M. |
collection | PubMed |
description | Routine manufacture, detonation and disposal of explosives in land and groundwater have resulted in complete pollution. Explosives are xenobiotic compounds, being toxic to biological systems, and their recalcitrance leads to persistence in the environment. The methods currently used for the remediation of explosive contaminated sites are expensive and can result in the formation of toxic products. The present study aimed to investigate the bacterial strains using the Biolog plates in the soil from the Riyadh community. The microbial strains were isolated using the spread plate technique and were identified using the Biolog method. In this study we have analyzed from bacterial families of soil samples, obtained from the different sites in 5 regions at Explosive Institute. Our results conclude that Biolog MicroPlates were developed for the rapid identification of bacterial isolates by sole-carbon source utilization and can be used for the identification of bacteria. Out of five communities, only four families of bacteria indicate that the microbial community lacks significant diversity in region one from the Riyadh community in Saudi Arabia. More studies are needed to be carried out in different regions to validate our results. |
format | Online Article Text |
id | pubmed-5415123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-54151232017-05-10 Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community Al-Dhabaan, Fahad Abdullah M. Bakhali, Ali Hassan Saudi J Biol Sci Original Article Routine manufacture, detonation and disposal of explosives in land and groundwater have resulted in complete pollution. Explosives are xenobiotic compounds, being toxic to biological systems, and their recalcitrance leads to persistence in the environment. The methods currently used for the remediation of explosive contaminated sites are expensive and can result in the formation of toxic products. The present study aimed to investigate the bacterial strains using the Biolog plates in the soil from the Riyadh community. The microbial strains were isolated using the spread plate technique and were identified using the Biolog method. In this study we have analyzed from bacterial families of soil samples, obtained from the different sites in 5 regions at Explosive Institute. Our results conclude that Biolog MicroPlates were developed for the rapid identification of bacterial isolates by sole-carbon source utilization and can be used for the identification of bacteria. Out of five communities, only four families of bacteria indicate that the microbial community lacks significant diversity in region one from the Riyadh community in Saudi Arabia. More studies are needed to be carried out in different regions to validate our results. Elsevier 2017-05 2016-02-01 /pmc/articles/PMC5415123/ /pubmed/28490963 http://dx.doi.org/10.1016/j.sjbs.2016.01.043 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Al-Dhabaan, Fahad Abdullah M. Bakhali, Ali Hassan Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community |
title | Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community |
title_full | Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community |
title_fullStr | Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community |
title_full_unstemmed | Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community |
title_short | Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community |
title_sort | analysis of the bacterial strains using biolog plates in the contaminated soil from riyadh community |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415123/ https://www.ncbi.nlm.nih.gov/pubmed/28490963 http://dx.doi.org/10.1016/j.sjbs.2016.01.043 |
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