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Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel

Programed cell death resembles a real nature active defense in Datura metel against TMV after three days of virus infection. This adaptive plant immune response was quantitatively assessed against Tomato Mosaic Virus infection by the following physiological markers; Chlorophyll-a (mg/g), Chlorophyll...

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Autores principales: Al-Huqail, Asma A., Aref, Nagwa M. Amin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415146/
https://www.ncbi.nlm.nih.gov/pubmed/28490959
http://dx.doi.org/10.1016/j.sjbs.2016.04.003
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author Al-Huqail, Asma A.
Aref, Nagwa M. Amin
author_facet Al-Huqail, Asma A.
Aref, Nagwa M. Amin
author_sort Al-Huqail, Asma A.
collection PubMed
description Programed cell death resembles a real nature active defense in Datura metel against TMV after three days of virus infection. This adaptive plant immune response was quantitatively assessed against Tomato Mosaic Virus infection by the following physiological markers; Chlorophyll-a (mg/g), Chlorophyll-b (mg/g), total protein (mg/g), hydrogen peroxide H(2)O(2) (μmol/100 mg), DNA (μg/100 mg), RNA (μg/100 mg), Salicylic acid (μg/g), and Comet Assays. Parameters were assessed for asymptomatic healthy and symptomatic infected detached leaves. The results indicated H(2)O(2) and Chlorophyll-a as the most potential parameters. Chlorophyll-a was considered the only significant predictor variant for the H(2)O(2) dependent variant with a P value of 0.001 and R-square of 0.900. The plant immune response was measured within three days of virus infection using the cutoff value of H(2)O(2) (⩽1.095 μmol/100 mg) and (⩽3.201 units) for the tail moment in the Comet Assay. Their percentage changes were 255.12% and 522.40% respectively which reflects the stress of virus infection in the plant. Moreover, H(2)O(2) showed 100% specificity and sensitivity in the symptomatic infected group using the receiver-operating characteristic (ROC). All tested parameters in the symptomatic infected group had significant correlations with twenty-five positive and thirty-one negative correlations where the P value was <0.05 and 0.01. Chlorophyll-a parameter had a crucial role of highly significant correlation between total protein and salicylic acid. Contrarily, this correlation with tail moment unit was (r = −0.930, P < 0.01) where the P value was <0.01. The strongest significant negative correlation was between Chlorophyll-a and H(2)O(2) at P < 0.01, while moderate negative significant correlation was seen for Chlorophyll-b where the P value < 0.05. The present study discloses the secret of the three days of rapid transient production of activated oxygen species (AOS) that was enough for having potential quantitative physiological parameters for defensive plant response toward the virus.
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spelling pubmed-54151462017-05-10 Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel Al-Huqail, Asma A. Aref, Nagwa M. Amin Saudi J Biol Sci Original Article Programed cell death resembles a real nature active defense in Datura metel against TMV after three days of virus infection. This adaptive plant immune response was quantitatively assessed against Tomato Mosaic Virus infection by the following physiological markers; Chlorophyll-a (mg/g), Chlorophyll-b (mg/g), total protein (mg/g), hydrogen peroxide H(2)O(2) (μmol/100 mg), DNA (μg/100 mg), RNA (μg/100 mg), Salicylic acid (μg/g), and Comet Assays. Parameters were assessed for asymptomatic healthy and symptomatic infected detached leaves. The results indicated H(2)O(2) and Chlorophyll-a as the most potential parameters. Chlorophyll-a was considered the only significant predictor variant for the H(2)O(2) dependent variant with a P value of 0.001 and R-square of 0.900. The plant immune response was measured within three days of virus infection using the cutoff value of H(2)O(2) (⩽1.095 μmol/100 mg) and (⩽3.201 units) for the tail moment in the Comet Assay. Their percentage changes were 255.12% and 522.40% respectively which reflects the stress of virus infection in the plant. Moreover, H(2)O(2) showed 100% specificity and sensitivity in the symptomatic infected group using the receiver-operating characteristic (ROC). All tested parameters in the symptomatic infected group had significant correlations with twenty-five positive and thirty-one negative correlations where the P value was <0.05 and 0.01. Chlorophyll-a parameter had a crucial role of highly significant correlation between total protein and salicylic acid. Contrarily, this correlation with tail moment unit was (r = −0.930, P < 0.01) where the P value was <0.01. The strongest significant negative correlation was between Chlorophyll-a and H(2)O(2) at P < 0.01, while moderate negative significant correlation was seen for Chlorophyll-b where the P value < 0.05. The present study discloses the secret of the three days of rapid transient production of activated oxygen species (AOS) that was enough for having potential quantitative physiological parameters for defensive plant response toward the virus. Elsevier 2017-05 2016-04-27 /pmc/articles/PMC5415146/ /pubmed/28490959 http://dx.doi.org/10.1016/j.sjbs.2016.04.003 Text en © 2016 Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Al-Huqail, Asma A.
Aref, Nagwa M. Amin
Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel
title Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel
title_full Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel
title_fullStr Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel
title_full_unstemmed Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel
title_short Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel
title_sort physiological parameters correlated with tomato mosaic virus inducing defensive response in datura metel
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415146/
https://www.ncbi.nlm.nih.gov/pubmed/28490959
http://dx.doi.org/10.1016/j.sjbs.2016.04.003
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