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The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking
In highly polarised cells, like fungal hyphae, early endosomes function in both endocytosis as well as long-distance transport of various cargo including mRNA and protein complexes. However, knowledge on the crosstalk between these seemingly different trafficking processes is scarce. Here, we demons...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415202/ https://www.ncbi.nlm.nih.gov/pubmed/28422978 http://dx.doi.org/10.1371/journal.pgen.1006734 |
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author | Haag, Carl Pohlmann, Thomas Feldbrügge, Michael |
author_facet | Haag, Carl Pohlmann, Thomas Feldbrügge, Michael |
author_sort | Haag, Carl |
collection | PubMed |
description | In highly polarised cells, like fungal hyphae, early endosomes function in both endocytosis as well as long-distance transport of various cargo including mRNA and protein complexes. However, knowledge on the crosstalk between these seemingly different trafficking processes is scarce. Here, we demonstrate that the ESCRT regulator Did2 coordinates endosomal transport in fungal hyphae of Ustilago maydis. Loss of Did2 results in defective vacuolar targeting, less processive long-distance transport and abnormal shuttling of early endosomes. Importantly, the late endosomal protein Rab7 and vacuolar protease Prc1 exhibit increased shuttling on these aberrant endosomes suggesting defects in endosomal maturation and identity. Consistently, molecular motors fail to attach efficiently explaining the disturbed processive movement. Furthermore, the endosomal mRNP linker protein Upa1 is hardly present on endosomes resulting in defects in long-distance mRNA transport. In conclusion, the ESCRT regulator Did2 coordinates precise maturation of endosomes and thus provides the correct membrane identity for efficient endosomal long-distance transport. |
format | Online Article Text |
id | pubmed-5415202 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54152022017-05-14 The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking Haag, Carl Pohlmann, Thomas Feldbrügge, Michael PLoS Genet Research Article In highly polarised cells, like fungal hyphae, early endosomes function in both endocytosis as well as long-distance transport of various cargo including mRNA and protein complexes. However, knowledge on the crosstalk between these seemingly different trafficking processes is scarce. Here, we demonstrate that the ESCRT regulator Did2 coordinates endosomal transport in fungal hyphae of Ustilago maydis. Loss of Did2 results in defective vacuolar targeting, less processive long-distance transport and abnormal shuttling of early endosomes. Importantly, the late endosomal protein Rab7 and vacuolar protease Prc1 exhibit increased shuttling on these aberrant endosomes suggesting defects in endosomal maturation and identity. Consistently, molecular motors fail to attach efficiently explaining the disturbed processive movement. Furthermore, the endosomal mRNP linker protein Upa1 is hardly present on endosomes resulting in defects in long-distance mRNA transport. In conclusion, the ESCRT regulator Did2 coordinates precise maturation of endosomes and thus provides the correct membrane identity for efficient endosomal long-distance transport. Public Library of Science 2017-04-19 /pmc/articles/PMC5415202/ /pubmed/28422978 http://dx.doi.org/10.1371/journal.pgen.1006734 Text en © 2017 Haag et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Haag, Carl Pohlmann, Thomas Feldbrügge, Michael The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking |
title | The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking |
title_full | The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking |
title_fullStr | The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking |
title_full_unstemmed | The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking |
title_short | The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking |
title_sort | escrt regulator did2 maintains the balance between long-distance endosomal transport and endocytic trafficking |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415202/ https://www.ncbi.nlm.nih.gov/pubmed/28422978 http://dx.doi.org/10.1371/journal.pgen.1006734 |
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