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Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood
Umbilical cord blood (CB) has emerged as an effective alternative donor source for hematopoietic stem cell transplantation. Despite this success, the prolonged duration of immune suppression following CB transplantation and the naiveté of CB T cells leave patients susceptible to viral infections. Ad...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415312/ https://www.ncbi.nlm.nih.gov/pubmed/28480300 http://dx.doi.org/10.1016/j.omtm.2017.02.001 |
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author | Dave, Hema Luo, Min Blaney, J.W. Patel, Shabnum Barese, Cecilia Cruz, Conrad Russell Shpall, Elizabeth J. Bollard, Catherine M. Hanley, Patrick J. |
author_facet | Dave, Hema Luo, Min Blaney, J.W. Patel, Shabnum Barese, Cecilia Cruz, Conrad Russell Shpall, Elizabeth J. Bollard, Catherine M. Hanley, Patrick J. |
author_sort | Dave, Hema |
collection | PubMed |
description | Umbilical cord blood (CB) has emerged as an effective alternative donor source for hematopoietic stem cell transplantation. Despite this success, the prolonged duration of immune suppression following CB transplantation and the naiveté of CB T cells leave patients susceptible to viral infections. Adoptive transfer of ex vivo-expanded virus-specific T cells from CB is both feasible and safe. However, the manufacturing process of these cells is complicated, lengthy, and labor-intensive. We have now developed a simplified method to manufacture a single culture of polyclonal multivirus-specific cytotoxic T cells in less than 30 days. It eliminates the need for a live virus or transduction with a viral vector, thus making this approach widely available and GMP-applicable to target multiple viruses. The use of overlapping PepMixes as a source of antigen stimulation enable expansion of the repertoire of the T cell product to any virus of interest and make it available as a third party “off the shelf” treatment for viral infections following transplantation. |
format | Online Article Text |
id | pubmed-5415312 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-54153122017-05-05 Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood Dave, Hema Luo, Min Blaney, J.W. Patel, Shabnum Barese, Cecilia Cruz, Conrad Russell Shpall, Elizabeth J. Bollard, Catherine M. Hanley, Patrick J. Mol Ther Methods Clin Dev Original Article Umbilical cord blood (CB) has emerged as an effective alternative donor source for hematopoietic stem cell transplantation. Despite this success, the prolonged duration of immune suppression following CB transplantation and the naiveté of CB T cells leave patients susceptible to viral infections. Adoptive transfer of ex vivo-expanded virus-specific T cells from CB is both feasible and safe. However, the manufacturing process of these cells is complicated, lengthy, and labor-intensive. We have now developed a simplified method to manufacture a single culture of polyclonal multivirus-specific cytotoxic T cells in less than 30 days. It eliminates the need for a live virus or transduction with a viral vector, thus making this approach widely available and GMP-applicable to target multiple viruses. The use of overlapping PepMixes as a source of antigen stimulation enable expansion of the repertoire of the T cell product to any virus of interest and make it available as a third party “off the shelf” treatment for viral infections following transplantation. American Society of Gene & Cell Therapy 2017-03-08 /pmc/articles/PMC5415312/ /pubmed/28480300 http://dx.doi.org/10.1016/j.omtm.2017.02.001 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Dave, Hema Luo, Min Blaney, J.W. Patel, Shabnum Barese, Cecilia Cruz, Conrad Russell Shpall, Elizabeth J. Bollard, Catherine M. Hanley, Patrick J. Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
title | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
title_full | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
title_fullStr | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
title_full_unstemmed | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
title_short | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
title_sort | toward a rapid production of multivirus-specific t cells targeting bkv, adenovirus, cmv, and ebv from umbilical cord blood |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415312/ https://www.ncbi.nlm.nih.gov/pubmed/28480300 http://dx.doi.org/10.1016/j.omtm.2017.02.001 |
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