Cargando…

A simple and effective protocol for fast isolation of human Tenon’s fibroblasts from a single trabeculectomy biopsy – a comparison of cell behaviour in different culture media

BACKGROUND: Human Tenon’s fibroblasts (HTFs) play a crucial role in wound healing. They cause postoperative scarring of the filtering bleb and are thus responsible for trabeculectomy failure. This study aimed to find an effective and fast protocol for HTF isolation from trabeculectomy biopsies. The...

Descripción completa

Detalles Bibliográficos
Autores principales:	Przekora, Agata, Zarnowski, Tomasz, Ginalska, Grazyna
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415760/ https://www.ncbi.nlm.nih.gov/pubmed/28536636 http://dx.doi.org/10.1186/s11658-017-0034-4

Ejemplares similares

Analysis of the impact of different schemes of preparation to trabeculectomy on the healing markers on the Tenon fibroblasts cultures
por: Piłat, Joanna, et al.
Publicado: (2023)

Trabeculectomy Tenon Advancement Technique May Reduce Bleb-Related Infections
por: Mambo, Yuki, et al.
Publicado: (2023)

Antimycobacterial Activity of Cinnamaldehyde in a Mycobacterium tuberculosis(H37Ra) Model
por: Sawicki, Rafal, et al.
Publicado: (2018)

Synthesis and in vitro antiproliferative and antibacterial activity of new thiazolidine-2,4-dione derivatives
por: Trotsko, Nazar, et al.
Publicado: (2017)

SPARC is expressed in scars of the Tenon’s capsule and mediates scarring properties of human Tenon’s fibroblasts in vitro
por: Fuchshofer, Rudolf, et al.
Publicado: (2011)

Autologous Transplantation of a Free Tenon's Graft for Repairing Excessive Bleb Leakage after Trabeculectomy: A Case Report
por: Kawai, Motofumi, et al.
Publicado: (2014)

Revision of trabeculectomy filtering blebs with mitomycin C: Long term results
por: Tulidowicz-Bielak, Maria, et al.
Publicado: (2016)

Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors
por: Kolmas, Joanna, et al.
Publicado: (2017)

Effect of Bevacizumab on Human Tenon's Fibroblasts Cultured from Primary and Recurrent Pterygium
por: Park, Young Min, et al.
Publicado: (2015)

Intracameral Bevacizumab Versus Sub-Tenon’s Mitomycin C as Adjuncts to Trabeculectomy: 3-Year Results of a Prospective Randomized Study
por: Kopsinis, Gerasimos, et al.
Publicado: (2021)

Effects of topical bevacizumab application on early bleb failure after trabeculectomy: observational case series
por: Klos-Rola, Justyna, et al.
Publicado: (2013)

The Effect of Combining Natural Terpenes and Antituberculous Agents against Reference and Clinical Mycobacterium tuberculosis Strains
por: Sieniawska, Elwira, et al.
Publicado: (2018)

Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential
por: Borkowski, Leszek, et al.
Publicado: (2021)

Predictive Value of Bleb Vascularity after Mitomycin C Augmented Trabeculectomy
por: Wlaź, Aleksandra, et al.
Publicado: (2020)

Autophagy and Mitochondrial Dysfunction in Tenon Fibroblasts from Exfoliation Glaucoma Patients
por: Want, Andrew, et al.
Publicado: (2016)

Effect of Vitamin D3 on Regulating Human Tenon's Fibroblasts Activity
por: Jia, Shuo, et al.
Publicado: (2021)

Anti-scarring effects of conbercept on human Tenon’s fibroblasts: comparisons with bevacizumab
por: Zuo, Lei, et al.
Publicado: (2023)

The Chemical and Biological Properties of Nanohydroxyapatite Coatings with Antibacterial Nanometals, Obtained in the Electrophoretic Process on the Ti13Zr13Nb Alloy
por: Bartmański, Michał, et al.
Publicado: (2021)

Positive Effect of Cold Atmospheric Nitrogen Plasma on the Behavior of Mesenchymal Stem Cells Cultured on a Bone Scaffold Containing Iron Oxide-Loaded Silica Nanoparticles Catalyst
por: Przekora, Agata, et al.
Publicado: (2020)

Role of Heat Shock Protein 47 in Transdifferentiation of Human Tenon's Fibroblasts to Myofibroblasts
por: Hong, Samin, et al.
Publicado: (2012)

Generation of induced pluripotent stem cells from human Tenon’s capsule fibroblasts
por: Deng, Fei, et al.
Publicado: (2012)

Nintedanib inhibits TGF-β-induced myofibroblast transdifferentiation in human Tenon’s fibroblasts
por: Lin, Xianchai, et al.
Publicado: (2018)

Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
por: Wei, Haiying, et al.
Publicado: (2019)

Elucidating the Therapeutic Potential of Cell-Penetrating Peptides in Human Tenon Fibroblast Cells
por: Chatterjee, Amit, et al.
Publicado: (2022)

Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface
por: Kalisz, Grzegorz, et al.
Publicado: (2021)

The Comparison between the Composition of 100% Autologous Serum and 100% Platelet-Rich Plasma Eye Drops and Their Impact on the Treatment Effectiveness of Dry Eye Disease in Primary Sjogren Syndrome
por: Wróbel-Dudzińska, Dominika, et al.
Publicado: (2023)

Transforming growth factor-β Does Not Induce Endothelin-1 Secretion in Primary Cultured Human Tenon's Fibroblasts
por: Hong, Samin, et al.
Publicado: (2008)

TGF-β-induced interleukin-6 participates in transdifferentiation of human Tenon’s fibroblasts to myofibroblasts
por: Seong, Gong Je, et al.
Publicado: (2009)

Brimonidine reduces TGF-beta-induced extracellular matrix synthesis in human Tenon’s fibroblasts
por: Hong, Samin, et al.
Publicado: (2015)

SPARC Levels Modulate the Capacity of Mitomycin to Inhibit the Proliferation of Human Tenon's Capsule Fibroblasts
por: Guo, Yuanyuan, et al.
Publicado: (2020)

The Pro-Fibrotic Behavior of Human Tenon’s Capsule Fibroblasts in Medically Treated Glaucoma Patients
por: Trelford, Charles B, et al.
Publicado: (2020)

LINC01605 knockdown induces apoptosis in human Tenon's capsule fibroblasts by inhibiting autophagy
por: Shang, Qifei, et al.
Publicado: (2022)

Sub-Tenon’s anesthesia: an update
por: Guise, Philip
Publicado: (2012)

Important Metabolites in Maintaining Folate Cycle, Homocysteine, and Polyamine Metabolism Associated with Ranibizumab Treatment in Cultured Human Tenon’s Fibroblasts
por: Munirah Md Noh, Siti, et al.
Publicado: (2019)

The Role of Focal Adhesion Kinase in the TGF-β-Induced Myofibroblast Transdifferentiation of Human Tenon's Fibroblasts
por: Hong, Samin, et al.
Publicado: (2012)

Suppression of Human Tenon Fibroblast Cell Proliferation by Lentivirus-Mediated VEGF Small Hairpin RNA
por: Li, Zhongqiu, et al.
Publicado: (2017)

MEK inhibition prevents TGF-β1-induced myofibroblast transdifferentiation in human tenon fibroblasts
por: Wen, Jiamin, et al.
Publicado: (2019)

Dihydroartemisinin Inhibits TGF-β-Induced Fibrosis in Human Tenon Fibroblasts via Inducing Autophagy
por: Wang, Xiaoyuan, et al.
Publicado: (2021)

Inhibition by a retinoic acid receptor γ agonist of extracellular matrix remodeling mediated by human Tenon fibroblasts
por: Liu, Yang, et al.
Publicado: (2015)

Inhibitory Effects of Angiotensin II Receptor Blockade on Human Tenon Fibroblast Migration and Reactive Oxygen Species Production in Cell Culture
por: Kim, Duri, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_vaf9r0blrusm7tmnnl1itcm701