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The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro
BACKGROUND: The primary human bone-derived cell culture technique is used as a model to study human osteogenesis. Compared to cell line cultures, primary osteoprogenitor and osteoblast cultures provide more complex information about osteogenesis, bone remodeling and regeneration than cell line cultu...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415846/ https://www.ncbi.nlm.nih.gov/pubmed/28536628 http://dx.doi.org/10.1186/s11658-016-0027-8 |
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author | Wrobel, Edyta Leszczynska, Joanna Brzoska, Edyta |
author_facet | Wrobel, Edyta Leszczynska, Joanna Brzoska, Edyta |
author_sort | Wrobel, Edyta |
collection | PubMed |
description | BACKGROUND: The primary human bone-derived cell culture technique is used as a model to study human osteogenesis. Compared to cell line cultures, primary osteoprogenitor and osteoblast cultures provide more complex information about osteogenesis, bone remodeling and regeneration than cell line cultures. METHODS: In this study, we isolated human bone-derived cells (HBDCs) and promoted their differentiation into osteoblasts. The following parameters were evaluated: cell number and viability, total protein expression, alkaline phosphatase activity, collagenous matrix production and osteogenic genes expression, i.e., gene coding for type I collagen and alkaline phosphatase. RESULTS: It was proved the results show that HBDCs intensively proliferate during the first 7 days of culture followed by differentiation accompanied by an increase in alkaline phosphatase activity. Moreover, it was observed that during the differentiation of HBDCs, the expression of integrin β1 increased. CONCLUSIONS: The process was also accompanied by changes in cell shape and rearrangement of the actin cytoskeleton and focal contacts containing FAK and the integrin β1 subunit. We suggest that the β1 integrin subunit may be a suitable new target in studies of the differentiation of primary human osteoblasts in culture. |
format | Online Article Text |
id | pubmed-5415846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54158462017-05-23 The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro Wrobel, Edyta Leszczynska, Joanna Brzoska, Edyta Cell Mol Biol Lett Research BACKGROUND: The primary human bone-derived cell culture technique is used as a model to study human osteogenesis. Compared to cell line cultures, primary osteoprogenitor and osteoblast cultures provide more complex information about osteogenesis, bone remodeling and regeneration than cell line cultures. METHODS: In this study, we isolated human bone-derived cells (HBDCs) and promoted their differentiation into osteoblasts. The following parameters were evaluated: cell number and viability, total protein expression, alkaline phosphatase activity, collagenous matrix production and osteogenic genes expression, i.e., gene coding for type I collagen and alkaline phosphatase. RESULTS: It was proved the results show that HBDCs intensively proliferate during the first 7 days of culture followed by differentiation accompanied by an increase in alkaline phosphatase activity. Moreover, it was observed that during the differentiation of HBDCs, the expression of integrin β1 increased. CONCLUSIONS: The process was also accompanied by changes in cell shape and rearrangement of the actin cytoskeleton and focal contacts containing FAK and the integrin β1 subunit. We suggest that the β1 integrin subunit may be a suitable new target in studies of the differentiation of primary human osteoblasts in culture. BioMed Central 2016-11-16 /pmc/articles/PMC5415846/ /pubmed/28536628 http://dx.doi.org/10.1186/s11658-016-0027-8 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Wrobel, Edyta Leszczynska, Joanna Brzoska, Edyta The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro |
title | The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro |
title_full | The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro |
title_fullStr | The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro |
title_full_unstemmed | The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro |
title_short | The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro |
title_sort | characteristics of human bone-derived cells (hbdcs) during osteogenesis in vitro |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415846/ https://www.ncbi.nlm.nih.gov/pubmed/28536628 http://dx.doi.org/10.1186/s11658-016-0027-8 |
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